2016
DOI: 10.1007/s10973-016-5764-4
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Application of isothermal titration calorimetry in evaluation of protein–nanoparticle interactions

Abstract: Nanoparticles (NPs) offer a number of advantages over small organic molecules for controlling protein behaviour inside the cell. Protein binding to the surface of NPs depends on their surface characteristics, composition and method of preparation (Mandal et al. in J Hazard Mater 248-249:238-245, 2013). It is important to understand the binding affinities, stoichiometries and thermodynamical parameters of NP-protein interactions in order to see which interaction will have toxic and hazardous consequences and t… Show more

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Cited by 30 publications
(25 citation statements)
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“…26 As a result, several protein corona studies employed ITC for characterizing the physicochemical properties of NP-protein interactions specifically. 4,11,13,16,[33][34][35][36][37][38][39][40][41][42][43][44][45][46][47] A review on the application of ITC in evaluation of nanoparticle-protein interactions by Omanovic-Miklicanin et al 48 and an excellent review elucidating the thermodynamics of NP-biomolecule interactions in general by Huang and Lau are available in the literature. 21 One big advantage of ITC studies on the protein corona is the unnecessity to separate the formed NP-protein complexes from the medium for further characterization.…”
Section: Investigating Nanoparticle-protein Interactions Via Itcmentioning
confidence: 99%
“…26 As a result, several protein corona studies employed ITC for characterizing the physicochemical properties of NP-protein interactions specifically. 4,11,13,16,[33][34][35][36][37][38][39][40][41][42][43][44][45][46][47] A review on the application of ITC in evaluation of nanoparticle-protein interactions by Omanovic-Miklicanin et al 48 and an excellent review elucidating the thermodynamics of NP-biomolecule interactions in general by Huang and Lau are available in the literature. 21 One big advantage of ITC studies on the protein corona is the unnecessity to separate the formed NP-protein complexes from the medium for further characterization.…”
Section: Investigating Nanoparticle-protein Interactions Via Itcmentioning
confidence: 99%
“…In essence, in all reported application fields of ITC, it is key to apply complementary techniques to analyze the nature of the interactions responsible for the thermal effects measured in ITC to fully interpret the data, e.g., interactions between proteins and nanoparticles, 11 , 12 where structural changes of proteins are of importance to study toxicity and understand the effect of nanoparticles on the proteins. By using ITC in combination with other analytical methods (e.g., dynamic light scattering (DLS), zeta-potential measurement, small-angle X-ray scattering (SAXS), fluorescence spectroscopy, dynamic force spectroscopy, quartz crystal microgravimetry), conformational changes in the protein can be studied.…”
Section: Introductionmentioning
confidence: 99%
“…By using ITC in combination with other analytical methods (e.g., dynamic light scattering (DLS), zeta-potential measurement, small-angle X-ray scattering (SAXS), fluorescence spectroscopy, dynamic force spectroscopy, quartz crystal microgravimetry), conformational changes in the protein can be studied. 11 , 12 The interactions of proteins with nanoparticles are often a combination of effects, such as hydrogen bonds, van der Waals interaction, and electrostatic interactions. 11 , 12 Fox et al 13 used ITC in combination with X-ray crystallography to show that the interaction mechanism of anions with the binding pocket of an anhydrase protein is based on ion-pair formation.…”
Section: Introductionmentioning
confidence: 99%
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“…A thermogram can be conveniently used for the characterization of a well-defined and relatively simple processes. 38 In a complex system, the titrant may trigger a sequence of events, as is the case in the presented studies. Specifically, upon titration, a compound may dissociate from MPs followed by its association with a protein or a lipid bilayer.…”
Section: Resultsmentioning
confidence: 74%