Application of Monoclonal Antibody, Specific for Intracellular <i>Orientia tsutsugamushi</i>, to Immunofluorescent Antibody Test for Determining Antibiotic Susceptibility

Mee-Kyung, Kim; Odgerel, Zagaa; Kim, Mi-Jeong; Chung, Moon-Hyun; Lim, Byung-Uk; Kang, Jae-Seung

doi:10.1111/j.1348-0421.2004.tb03475.x

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…This result is consistent with that of our previous study, in which we determined the antibiotic susceptibility of both strains using IFA (7). However, strain AFSC-4 is reported to be doxycycline-resistant and its MIC was reported to be 0.25 µg/mL (5).…”

Section: Discussionsupporting

confidence: 93%

“…In a previous study, we reported the characteristics of a monoclonal antibody (MAb), M686-13, that reacts exclusively with intracellular O. tsutsugamushi , and its application in an immunofluorescence assay (IFA) to determine antibiotic susceptibility (6, 7). However, that method requires the manual count of bacterial particles or computer-assisted scanning.…”

Section: Introductionmentioning

confidence: 99%

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Improved Antibiotic Susceptibility Test of Orientia tsutsugamushi by Flow Cytometry Using Monoclonal Antibody

2007

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Orientia tsutsugamushi causes scrub typhus, which is endemic in many countries in the Asia-Pacific region including Korea. Recent emergence of doxycycline-resistant strains from Thailand has underlined the importance of the susceptibility tests of O. tsutsugamushi to antibiotics. To improve the flow cytometric technique for the susceptibility test, we applied a monoclonal antibody (MAb) in the quantification of O. tsutsugamushi. With using MAb FS15, we determined the doxycycline susceptibility of two strains, Boryong and AFSC-4 strain which is reported to be doxycycline-sensitive and resistant, respectively. The growth of both strains was inhibited to below 10% of the control in the presence of 0.1 µg/mL or higher concentrations of doxycycline. We suggest that our approach is more quantitative and reproducible than the conventional microscopic methods.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Improved Antibiotic Susceptibility Test of Orientia tsutsugamushi by Flow Cytometry Using Monoclonal Antibody

2007

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“…in insect cells (22). Thus, this strikingly low MIC of CorA was specific for O. tsutsugamushi, and it is lower than the MICs observed for any other antimicrobial substance that has been so far tested against O. tsutsugamushi (32)(33)(34)(35)(36). A possible exception may be the tetracycline derivative tigecycline, which was recently shown to inhibit growth in vitro of O. tsutsugamushi (37), but which awaits susceptibility testing in vivo.…”

Section: Discussionmentioning

confidence: 93%

Orientia tsutsugamushi Is Highly Susceptible to the RNA Polymerase Switch Region Inhibitor Corallopyronin A In Vitro and In Vivo

Kock

Hauptmann

Osterloh

et al. 2018

Antimicrob Agents Chemother

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Scrub typhus is a potentially lethal infection caused by the obligate intracellular bacterium Reports on the emergence of doxycycline-resistant strains highlight the urgent need to develop novel antiinfectives against scrub typhus. Corallopyronin A (CorA) is a novel α-pyrone compound synthesized by the myxobacterium that was characterized as a noncompetitive inhibitor of the switch region of the bacterial RNA polymerase (RNAP). We investigated the antimicrobial action of CorA against the human-pathogenic Karp strain of and The MIC of CorA against was remarkably low (0.0078 μg/ml), 16-fold lower than that against In the lethal intraperitoneal mouse infection model, a minimum daily dose of 100 μg CorA protected 100% of infected mice. Two days of treatment were sufficient to confer protection. In contrast to BALB/c mice, SCID mice succumbed to the infection despite treatment with CorA or tetracycline, suggesting that antimicrobial treatment required synergistic action of the adaptive immune response. Similar to tetracycline, CorA did not prevent latent infection of However, latency was not caused by acquisition of antimicrobial resistance, since reisolated from latently infected BALB/c mice remained fully susceptible to CorA. No mutations were found in the CorA-binding regions of the β and β' RNAP subunit genes and Inhibition of the RNAP switch region of by CorA is therefore a novel and highly potent target for antimicrobial therapy for scrub typhus.

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Orientia tsutsugamushiand Scrub Typhus

Watt¹,

Kantipong²

2007

Infectious Disease and Therapy

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Application of Monoclonal Antibody, Specific for Intracellular Orientia tsutsugamushi, to Immunofluorescent Antibody Test for Determining Antibiotic Susceptibility

Cited by 5 publications

References 21 publications

Improved Antibiotic Susceptibility Test of Orientia tsutsugamushi by Flow Cytometry Using Monoclonal Antibody

Improved Antibiotic Susceptibility Test of Orientia tsutsugamushi by Flow Cytometry Using Monoclonal Antibody

Orientia tsutsugamushi Is Highly Susceptible to the RNA Polymerase Switch Region Inhibitor Corallopyronin A In Vitro and In Vivo

Orientia tsutsugamushiand Scrub Typhus

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