Application of Oxidative Stress to a Tissue-Engineered Vascular Aging Model Induces Endothelial Cell Senescence and Activation

Liu

Front. Cardiovasc. Med.

et al. 2022

The aging of endothelial cells plays a critical role in the development of age-related vascular disease. We established a model of endothelial premature senescence by application of Advanced oxidation protein products (AOPPs) modified bovine serum albumin (AOPP-BSA) in human umbilical vein endothelial cells (HUVECs). This cellular senescence was accompanied with endothelial barrier dysfunction and angiogenesis impairment. It was further revealed that these senescent HUVECs underwent apoptosis evasion and the receptor for advanced glycation endproducts (RAGE) played a role in these processes. The AOPP-induced senescence was regulated by the state of autophagy in HUVECs. We further proved that AOPP-BSA attenuated the autophagy of HUVECs, led to p53 SUMOylation at K386, resulting in endothelial senescence. We also established the animal model of vascular senescence by using ApoE−/− mice fed with high-fat diet plus daily injection of AOPP-BSA to verify the role of p53 SUMOylation in vascular senescence. Combined with intraperitoneal injection of rapamycin, the effect of autophagy on AOPP-induced p53 SUMOylation was also confirmed in vivo. Our data indicates that p53 SUMOylation at K386 plays an important role in AOPP-induced endothelial senescence and apoptosis evasion, suggesting that p53 K386 SUMOylation may serve as a potential therapeutic target in protecting against vascular senescence.

Section: Discussionmentioning

confidence: 99%

p53 SUMOylation Mediates AOPP-Induced Endothelial Senescence and Apoptosis Evasion

Liu

Front. Cardiovasc. Med.

et al. 2022

Front. Cardiovasc. Med.

“…Apart from PCSK9, the same group also explored the effect of oxidative stress on inflammation and senescence on vascular cells using a 2-layered TEBV composed of endothelium and fibroblast layers. The data generated from the model suggested that oxidative stress promoted atherosclerosis by increasing vascular cell inflammation (165)(166)(167). Likely, Chen et al developed a 2layered EC-SMC TEBV.…”

Section: In Vitro 3d Vessel Based Systems Tissue-engineered Blood Vessels (Tebvs)mentioning

confidence: 99%

Recent Progress in in vitro Models for Atherosclerosis Studies

Zhang

Millican

Lynd

et al. 2022

Atherosclerosis is the primary cause of hardening and narrowing arteries, leading to cardiovascular disease accounting for the high mortality in the United States. For developing effective treatments for atherosclerosis, considerable efforts have been devoted to developing in vitro models. Compared to animal models, in vitro models can provide great opportunities to obtain data more efficiently, economically. Therefore, this review discusses the recent progress in in vitro models for atherosclerosis studies, including traditional two-dimensional (2D) systems cultured on the tissue culture plate, 2D cell sheets, and recently emerged microfluidic chip models with 2D culture. In addition, advanced in vitro three-dimensional models such as spheroids, cell-laden hydrogel constructs, tissue-engineered blood vessels, and vessel-on-a-chip will also be covered. Moreover, the functions of these models are also summarized along with model discussion. Lastly, the future perspectives of this field are discussed.

“…Hydrogen peroxide (H 2 O 2 ), a major product of human metabolism, is an important indicator for monitoring and maintaining physiological balance of biological tissues in vivo. Unbalanced H 2 O 2 in the body leads to serious diseases, such as neurodegenerative Alzheimer's, [1] aging, [2] atherogenesis, [3] infarction, [4] cardiovascular diseases [5] and cancer [6] . Because of its great oxidizing capacity, H 2 O 2 is also extensively employed for sterilization and oxidative bleaching in the industrial, medicinal, and environmental protection fields.…”

Section: Introductionmentioning

confidence: 99%

Two‐Dimensional Nitrogen‐Doped Ti₃C₂ Promoted Catalysis Performance of Silver Nanozyme for Ultrasensitive Detection of Hydrogen Peroxide

Qiu

Zhu

et al. 2022

ChemElectroChem

Silver nanoparticles (AgNPs)-based nanozyme sensors are gaining attention for rapid on-site H 2 O 2 detection, which is beneficial to disease diagnosis and environmental monitoring. However, the severe agglomeration of AgNPs on the electrodes significantly reduced electrochemical catalytic activity. In this work, we fabricated N-doped Ti 3 C 2 MXene (named Ag/NÀ Ti 3 C 2 ) deposited with three-dimensional flower-like AgNPs to achieve ultrasensitive H 2 O 2 detection. N doping strategy is employed to improve the conductivity of MXene, and the corresponding catalytic activity of Ag/NÀ Ti 3 C 2 is enhanced by optimizing the growth process and morphology. The Ag/NÀ Ti 3 C 2 /glassy carbon electrode (Ag/N-Ti 3 C 2 /GCE) sensor exhibited a wide H 2 O 2 detection range (0.05-35 mM), a low limit detection (1.53 μM), and 3.1 % relative standard deviation in 50 repeated cyclic voltammetry measurements. The results above indicate that catalytic activity of metal nanozymes could be influenced by the substrate and both materials jointly determine the sensor's performance. As a result, N-doped MXene is an attractive candidate for biological sensing and other electrocatalytic applications.