Application of Plackett-Burman Experimental Design for Lipase Production by <i>Aspergillus niger</i> Using Shea Butter Cake

Salihu, Aliyu; Bala, Manju; Bala, Shuaibu Mallam

doi:10.5402/2013/718352

Cited by 28 publications

(18 citation statements)

References 24 publications

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“…Kynclova et al (1995) screened various hydrolytic enzymes to meet the special demands. Among the screened microorganisms, fungal lipases turned out to be the best source for lipase as they are thermally stable with high turnover number and are presently receiving attention due to the easy recovery of extracellular enzymes (Kynclova et al 1995;Mahadik et al 2002;Salihu et al 2013;Fleuri et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…These agricultural residues are being generated in huge quantities in developing countries and problems associated with their disposal is also an important environmental concern. Thus, utilizing these residues as nutrient source for enzyme production will reduce overall production cost (Salihu et al 2013). Hence, the selection of an appropriate substrate is an essential step during the production of enzymes in SSF.…”

Section: Introductionmentioning

confidence: 99%

“…Hence, the selection of an appropriate substrate is an essential step during the production of enzymes in SSF. Various agro-industrial residues such as wheat bran, soybean cake, rice husk, gingelly oil cake, olive oil cake, sugar cane bagasse, babassu oil cake, and sheanut cake were studied (Salihu et al 2013(Salihu et al , 2016Fleuri et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Production and optimization of lipase using Aspergillus niger MTCC 872 by solid-state fermentation

et al. 2019

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Background: Lipases are serine hydrolases that degrade triglycerides, an attribute that treasures wide applications in biodiesel production, detergent, chemical industries, etc. The most sought after the application is in the high quality and economical production of biodiesel under mild reaction conditions and simplified product separation. For the said application, fungal lipases are ideal catalysts that could effectively catalyze esterification and transesterification reactions with their specific ability to release fatty acids from 1, 3 positions of acylglycerols. Results: In the present work, to facilitate bulk synthesis, lipase production using Aspergillus niger MTCC 872 was studied by solid-state fermentation (SSF). The chosen fungal strain was evaluated for lipase production using a mixture of agroindustrial substrates viz. rice husk, cottonseed cake, and red gram husk in various combinations at flask level. Tri-substrate mixture (rice husk, cottonseed cake, and red gram husk) combined in the ratio of 2:1:1 has shown the maximum lipase activity 28.19 U/gds at optimum cultivation conditions of temperature 40°C, moisture content 75% (v/w), pH 6.0 and initial spore concentration of 5.4 million spores per mL. Further studies were performed for scale-up of lipase from flask level to lab scale using tray fermenter. Lipase activity was found to be 24.38 U/gds and 21.62 U/gds for 100 g and 1000 g substrate respectively. Conclusion: This is the first report on the production of lipase from Aspergillus niger MTCC 872 using tri-substrate mixture of rice husk (RH), cottonseed cake (CSC), and red gram husk (RGH). Moreover, comparison between individual, binary, and tri-substrate mixture was carried out for which the highest lipase activity was observed for tri-substrate mixture. In addition, comparable results were found when scale-up was performed using tray fermenter. Thus, the current work signifies usage of agro-industrial residues as substrates for enzyme production by solid-state fermentation process as an effective alternative to submerged fermentation for industrial applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production and optimization of lipase using Aspergillus niger MTCC 872 by solid-state fermentation

et al. 2019

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“…Using SNC as the main substrate for lipase production may boost its economic value, which can be better than its current potential as an animal feed ingredient. This study involves the use of the one-factor-at-atime (OFAT) method and statistical experimental design (face-centred central composite design (FCCCD)) to optimize the lipase production by A. niger based on the identified medium components affecting the lipase production as previously reported by Salihu et al [21]. Thus, A. niger was selected as a potential strain because of its robustness, high yield of extracellular enzymes, non-pathogenicity, non-toxicity and GRAS (generally regarded as safe) nature as accorded by US Food and Drug Administration.…”

Section: Introductionmentioning

confidence: 99%

Lipase production by Aspergillus niger using sheanut cake: An optimization study

Salihu

Bala

Alam

2016

Journal of Taibah University for Science

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The effects of medium components affecting lipase production by Aspergillus niger AS-02 using sheanut cake (SNC) were studied based on the one-factor-at-a-time (OFAT) method and the face-centred central composite design (FCCCD). Of the six medium components analysed by OFAT, three (Tween-80, (NH 4) 2 SO 4 and Na 2 HPO 4) were subjected to FCCCD. Under optimized conditions, the obtained lipase production showed good agreement between the experimental (49.37 U/g) and predicted (48.81 U/g) yields, with a coefficient of determination (R 2) of 0.96. The optimum medium components were 1.0% (v/w) Tween-80, 0.35% (w/w) (NH 4) 2 SO 4 and 0.40% (w/w) Na 2 HPO 4. The findings in this study showed that, in addition to lipase production, the fermented SNC had reduced tannin and saponin contents, which are the major components that limit the use of SNC in feed formulations.

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“…In order to reduce the cost of enzyme production, the use of low‐cost substrates is advisable. Lipid residues and low‐cost renewable carbon sources such as brans (e.g., wheat, rice, soybean, and barley), oil cakes (soybean, olive, sesame, and babassu), bagasse (sugarcane), and oils (waste cooking oil, non‐edible seed oils) have been the focus of studies in this direction.…”

Section: Introductionmentioning

confidence: 99%

Comparison of lipase production on crambe oil and meal by Fusarium sp. (Gibberella fujikuroi complex)

Oliveira

Coradi

Attili‐Angelis

et al. 2013

Euro J Lipid Sci & Tech

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This study provides a comparison of fermentation conditions for the production lipases by Fusarium sp. (Gibberella fujikuroi complex) isolate FCLA-MA-41 in submerged (SmF) and solid-state fermentation (SSF) using agro-industrial residues. To assess SmF, a univariate study of seven carbon sources (crambe, corn, linseed, olive, palm (dendê), soybean oils and chicken fat) and four nitrogen sources (ammonium sulfate, sodium nitrate, urea, yeast extract) was performed. The relationship among the concentrations of carbon and nitrogen sources, Triton X-100 and yeast extract was studied using a Central Composite Design (CCD). To assess SSF, different residues (sugarcane bagasse) and oil-seed meals (castorbean, corn, crambe, soybean) with additional supplements added (minimum salts, seed oils) were examined. Based on the CCD matrix, a medium containing crambe oil (17.5 mL/L), Triton X-100 (5 g/L), ammonium sulfate (5 g/L) and yeast extract (1 g/L) was proposed, resulting in a lipase titer of 3.0 AE 0.25 U/mL. The cost/production relationship was 7.73% less expensive than SmF with corn oil. SSF produced a maximum lipase titer of 5.0 AE 0.25 U/gds on crambe meal moistened with phosphate buffer. The best cost/enzyme activity estimate was for SSF with crambe meal as substrate in only distilled water (87.27% less expensive than SmF).

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Application of Plackett-Burman Experimental Design for Lipase Production by Aspergillus niger Using Shea Butter Cake

Cited by 28 publications

References 24 publications

Production and optimization of lipase using Aspergillus niger MTCC 872 by solid-state fermentation

Production and optimization of lipase using Aspergillus niger MTCC 872 by solid-state fermentation

Lipase production by Aspergillus niger using sheanut cake: An optimization study

Comparison of lipase production on crambe oil and meal by Fusarium sp. (Gibberella fujikuroi complex)

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