Application of real-time PCR for the differentiation of<i>Entamoeba histolytica</i>and<i>E. dispar</i>in cyst-positive faecal samples from 130 immigrants living in Spain

Gutiérrez-Cisneros, María José; Cogollos, R; López‐Vélez, Rogelio; Martín‐Rabadán, Pablo; Martínez-Ruiz, Rocío; Subirats, Mercedes; Merino, F.; Fuentes, Isabel

doi:10.1179/136485910x12607012373759

Cited by 35 publications

(27 citation statements)

References 15 publications

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“…The ability to differentiate between pathogenic E. histolytica and non-pathogenic Entamoeba species is clinically significant. Results concur with a prevalence study of Entamoeba in immigrants in Spain that showed that the majority of cyst-positive samples detected by microscopy were E. dispar and do not require treatment 5 .…”

Section: Discussionsupporting

confidence: 77%

Protozoa PCR: boon or bane

Pham¹,

Sheorey²

2016

Microbiol. Aust.

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Parasite detection in faeces has traditionally been performed by microscopy, a procedure that is labour-intensive and highly specialised. In addition, identification by microscopy based on morphological features alone is subjective and prone to wide variability. Although enzyme immunoassays (EIA) of high sensitivity have been developed1 they can detect only a limited range of pathogens. Given these factors the introduction of Polymerase Chain Reaction (PCR) into the routine diagnostic laboratory has improved parasite detection rates2. The ability to multiplex has enabled the detection of multiple targets from a single sample and provides an objective alternative to identification by morphology.

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Section: Discussionsupporting

confidence: 77%

Protozoa PCR: boon or bane

Pham¹,

Sheorey²

2016

Microbiol. Aust.

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“…Hence, we indicated that of 23 positive samples in multiplex PCR test, 13 and 10 samples were positive for E. dispar and E. histolytica, respectively. Unlike our results, previous studies indicated that E. dispar was approximately ten times more common in stool samples than E. histolytica [19,21].…”

Section: Discussioncontrasting

confidence: 56%

Entamoeba spp. diagnosis in patients with inflammatory diarrhea by staining, copro-antigen ELISA and multiplex PCR methods

Gharibi¹,

Kazemi²,

Tavalla³

2017

APJTD

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“…Both conventional and real-time PCRs have proven to be more sensitive and specific than microscopic examination for the detection of E. histolytica and E. dispar in single stool samples (455,460). In developed settings, PCR is more useful for detection of E. histolytica in stools than antigen detection tests due to the higher sensitivities observed for PCR and the reduced chance of cross-reactivity with other Entamoeba species (167,397,460). However, PCR has yet to become the mainstream for the detection of parasites in the clinical laboratory, even in developed countries, due to the need for specialized equipment, dedicated molecular areas, specified workflow, and associated costs.…”

Section: Entamoeba Speciesmentioning

confidence: 99%

Enteric Protozoa in the Developed World: a Public Health Perspective

et al. 2012

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SUMMARY Several enteric protozoa cause severe morbidity and mortality in both humans and animals worldwide. In developed settings, enteric protozoa are often ignored as a cause of diarrheal illness due to better hygiene conditions, and as such, very little effort is used toward laboratory diagnosis. Although these protozoa contribute to the high burden of infectious diseases, estimates of their true prevalence are sometimes affected by the lack of sensitive diagnostic techniques to detect them in clinical and environmental specimens. Despite recent advances in the epidemiology, molecular biology, and treatment of protozoan illnesses, gaps in knowledge still exist, requiring further research. There is evidence that climate-related changes will contribute to their burden due to displacement of ecosystems and human and animal populations, increases in atmospheric temperature, flooding and other environmental conditions suitable for transmission, and the need for the reuse of alternative water sources to meet growing population needs. This review discusses the common enteric protozoa from a public health perspective, highlighting their epidemiology, modes of transmission, prevention, and control. It also discusses the potential impact of climate changes on their epidemiology and the issues surrounding waterborne transmission and suggests a multidisciplinary approach to their prevention and control.

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Application of real-time PCR for the differentiation ofEntamoeba histolyticaandE. disparin cyst-positive faecal samples from 130 immigrants living in Spain

Cited by 35 publications

References 15 publications

Protozoa PCR: boon or bane

Protozoa PCR: boon or bane

Entamoeba spp. diagnosis in patients with inflammatory diarrhea by staining, copro-antigen ELISA and multiplex PCR methods

Enteric Protozoa in the Developed World: a Public Health Perspective

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