Application of Retention Factors in Affinity Electrokinetic Chromatography and Capillary Electrophoresis

Østergaard, Jesper

doi:10.2116/analsci.23.489

Cited by 10 publications

(8 citation statements)

References 30 publications

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“…ACE was used to examine the interaction between fluoroquinolones and HSA 28. As noted in the Ostergaard paper,29 the authors incorrectly applied the well‐known equation for the retention factor ( k ) to capillary electrophoretic affinity studies. Fluorescence quenching technique was also used to examine the interaction and was compared to the CE method.…”

Section: Discussionmentioning

confidence: 99%

“…Recent work28 incorrectly applied the well‐known equation for the retention factor ( k = ( t r − t 0 / t 0 )) to capillary electrophoretic affinity studies assuming a two‐phase system. Ostergaard has carefully examined why the use of the retention factor in determining affinity parameters is not appropriate 29. The retention factor in electrokinetic chromatography can be expressed in terms of migration times that can be related to electrophoretic mobility (Eq.…”

Section: Discussionmentioning

confidence: 99%

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Recent Advances in Affinity Capillary Electrophoresis (2007)

Liu

Dahdouh

Salgado

et al. 2009

Journal of Pharmaceutical Sciences

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Recent Advances in Affinity Capillary Electrophoresis (2007)

Liu

Dahdouh

Salgado

et al. 2009

Journal of Pharmaceutical Sciences

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“…Optimum conditions have been elaborated using ITP with conductometric detection, in spite of small differences of electrophoretic mobility of analyzed compounds. New terminating electrolytes were used: solutions of 4 mg/L 1 1 n = 5, the samples were analyzed twice 2 The sample was enriched with 1.5 mL of a solution containing 1 mg/mL of examined ion, n = 5 3 Correlation coefficient above 0.97 4 Calculated from the limit of identification and coefficients of the calibration curve 1-(N-morpholiniomethyl)spirobi(1-sila-2,5-dioxacyclopentan-3-on)ate and 4,4 -bis{1-(perhydroazepiniomethyl)spirobi(1-sila-2,5-dioxacyclopentan-3-on)]ate}. Optimum conditions of separation and determination of these compounds have been elaborated with success also using the HPLC technique.…”

Section: Discussionmentioning

confidence: 99%

“…Capillary ITP, which was used to separate chosen compounds and their isomers (2)(3)(4) can serve as an example. This technique is applied more and more often in analytic laboratories.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Biologically Active Stilbene Derivatives

Chrząścik¹

2009

Critical Reviews in Analytical Chemistry

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An attempt of synthesis of information concerning chosen stilbene derivatives has been made. These compounds show biological activity that is very important to the whole ecosystem. There are known such derivatives of stilbene that are characterized by a confirmed anti-cancer, antibacterial and fungistatic activity. Some of them have positive influences on the cardiovascular system. Some others have properties of liquid crystals and the ability of complexing. Stilbenes and their various derivatives are still arousing immense interest. High hopes are connected with the possibility of more and wider pharmacological use and curing different diseases.

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Analysis of ruthenium anticancer agents by MEEKC‐UV and MEEKC–ICP‐MS: Impact of structural motifs on lipophilicity and biological activity

et al. 2018

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We present here the first comprehensive study on the lipophilicity of ruthenium anticancer agents encompassing compounds with broad structural diversity, ranging from octahedral Ru (azole) through to Ru (arene) complexes. MEEKC was used to determine the capacity factors of the Ru complexes, and after a complex peak was unambiguously assigned using MEEKC-ICP-MS, the results were validated through comparison with the log P determined by octanol/water partitioning experiments. Correlation of the two data sets demonstrated a close relationship despite the limited structural overlap of the compounds studied. The capacity factors found by MEEKC allowed for the clustering of complexes based on their structure and this could be used to rationalize the observed cytotoxicity in the human colon carcinoma HCT116 cell line. It was demonstrated that rather than modification of the mono- or bidentate coordinated ligands much tighter control over a complexes lipophilic properties could be achieved through modification of the Ru(arene) ligand, with minimal detriment to cytotoxicity. This demonstrates the flexibility and potential of the Ru piano-stool scaffold. MEEKC proved to be a highly efficient means of screening the anticancer potential of preclinical ruthenium complex candidates for their lipophilic properties and correlate them with their biological activity and structural properties.

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Application of Retention Factors in Affinity Electrokinetic Chromatography and Capillary Electrophoresis

Cited by 10 publications

References 30 publications

Recent Advances in Affinity Capillary Electrophoresis (2007)

Recent Advances in Affinity Capillary Electrophoresis (2007)

Analysis of Biologically Active Stilbene Derivatives

Analysis of ruthenium anticancer agents by MEEKC‐UV and MEEKC–ICP‐MS: Impact of structural motifs on lipophilicity and biological activity

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