Application of SRIXE and XANES to the determination of the oxidation state of iron in prostate tissue sections

Kwiatek, Wojciech M.; Hanson, A.L.; Paluszkiewicz, C.; Gałka, Marek; Gajda, Mariusz; Cichocki, T

doi:10.1016/s0925-8388(03)00566-8

Cited by 32 publications

(30 citation statements)

References 13 publications

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“…They were displayed as typical components and markers of cell-rich areas and they were considered as tissue structure tracers. 53 In summary, micro-XRF spectrometry was shown to be an efficient tool for evaluation of atherosclerotic plaque elemental content. The use of this method provides a novel insight into the morphological and physicochemical properties of the atherosclerotic plaques and may be used in further investigations aimed at characterization of the effects of pharmacological and dietary treatment on elemental content of atherosclerotic plaques in apoE/LDLR / mice.…”

Section: Discussionmentioning

confidence: 99%

Distribution of selected elements in atherosclerotic plaques of apoE/LDLR‐double knockout mice assessed by synchrotron radiation‐induced micro‐XRF spectrometry

Gajda

Banaś

et al. 2008

X-Ray Spectrometry

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Section: Discussionmentioning

confidence: 99%

Distribution of selected elements in atherosclerotic plaques of apoE/LDLR‐double knockout mice assessed by synchrotron radiation‐induced micro‐XRF spectrometry

Gajda

Banaś

et al. 2008

X-Ray Spectrometry

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“…The comparison of our results with published data for Br, Fe, Rb, Sr, and Zn mass fraction in normal and cancerous prostate tissue [32][33][34][35][36][37][38][39][40][41][42][43][44] is shown in Table 3.…”

Section: Resultsmentioning

confidence: 85%

Trace Element Contents in Adenocarcinoma of Human Prostate Investigated by Energy Dispersive X-Ray Fluorescent Analysis

Zaichick¹,

Zaichick²

2016

J Adenocarcinoma

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Background: Adenocarcinoma of prostate is an internationally important health problem of the man, particularly in developed countries. The aim of this exploratory study was to evaluate whether significant changes in the prostatic tissue levels of Zn, as an androgen dependent trace element and Br, Fe, Rb, and Sr, as androgen independent trace elements, exist in the malignantly transformed prostate. Methods and findings:Prostatic tissue levels of Br, Fe, Rb, Sr, and Zn as well as ratio of Zn content to other trace element contents were prospectively evaluated in 35 patients with adenocarcinoma and 37 healthy male inhabitants. Measurements were performed using 109 Cd radionuclide-induced energy dispersive X-ray fluorescent analysis Tissue samples were divided into two portions. One was used for morphological study while the other was intended for trace element analysis. Mean values ± standard error of means (M ± SΕΜ) for mass fraction (mg/kg on dry mass basis) of trace element in the normal tissue was: Br 38.8 ± 5.6, Fe 118 ± 8, Rb 16.3 ± 1.1, Sr 2.5 ± 0.4, and Zn 1154 ± 119, respectively. M ± SΕΜ for ratio of mass fractions were: Zn/Br 38.7 ± 6.4, Zn/Fe 11.2 ± 1.3, Zn/Rb 71.7 ± 9.0 and Zn/Sr 534 ± 83, respectively. The contents of Rb and Zn were significantly lower (approximately 2 and 8 times, respectively) and those of Br and Sr was significantly higher (2.7 and 2.2 times, respectively) in cancerous tissues than in normal tissues. Conclusions:There are great changes in trace element contents and their relationships in the malignantly transformed tissue of prostate.

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“…8 There is a consensus that the best way to prepare samples for topographic and quantitative elemental analysis by the mSR-XRF technique is to use fresh tissue samples cut using cryostat or cryomicrotome, and then being freeze-dried. [9][10][11][12][13] Therefore, in this work we used cryofixation by freezing in isopentane, a cryoliquid, in order to minimize ice crystal formation. 14 Cryofixation, during which tissue and cells are rapidly frozen to preserve their integrity, is considered to be the best way of preparing samples for both high resolution morphological studies and analytical studies in which the measurement of elemental concentration is meaningful.…”

Section: Sample Preparationsmentioning

confidence: 99%

Topographic Trace-Elemental Analysis in the Brain of Wistar Rats by X-ray Microfluorescence with Synchrotron Radiation

et al. 2008

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Knowledge about the spatial distribution and the local concentration of trace elements in tissues is of great importance, since trace elements are involved in many biological functions of living organisms. However, there are few methods available to measure the spatial (two (three)-dimensional) elemental distribution in animal brain. X-ray microfluorescence with synchrotron radiation is a multielemental mapping technique, which was used in this work to determine the topographic of iron, zinc and copper in coronal sections of female Wistar rats of different ages. Young (14 days old) and middle-aged (20 months old) rats (n = 8) were analyzed. The measurements were carried out at the XRF beam line at the Synchrotron Light National Laboratory (Campinas, Brazil). Two-dimensional scanning was performed in order to study the tendency of elemental concentration variation. The acquisition time for each pixel was 10 s/step and the step size was 300 mm/step in both directions. It was observed that the iron distribution was more conspicuous in the cortical area, thalamus and bellow the thalamus. On the other hand, the zinc distribution was more pronounced in the hippocampus. The iron, copper and zinc levels increased with advancing age. Therefore, this study reinforces the idea that these elements are involved in the chemical mechanisms of the brain that induce some neurological diseases, since they are also present in high levels in specific areas of the brain, such as the hippocampus and the substantia nigra of patients with these disorders.

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Application of SRIXE and XANES to the determination of the oxidation state of iron in prostate tissue sections

Cited by 32 publications

References 13 publications

Distribution of selected elements in atherosclerotic plaques of apoE/LDLR‐double knockout mice assessed by synchrotron radiation‐induced micro‐XRF spectrometry

Distribution of selected elements in atherosclerotic plaques of apoE/LDLR‐double knockout mice assessed by synchrotron radiation‐induced micro‐XRF spectrometry

Trace Element Contents in Adenocarcinoma of Human Prostate Investigated by Energy Dispersive X-Ray Fluorescent Analysis

Topographic Trace-Elemental Analysis in the Brain of Wistar Rats by X-ray Microfluorescence with Synchrotron Radiation

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