Application of the Ceditest® FMDV type O and FMDV-NS enzyme-linked immunosorbent assays for detection of antibodies against <i>Foot-and-mouth disease virus</i> in selected livestock and wildlife species in Uganda

Ayebazibwe, Chrisostom; Mwiine, Frank Norbert; Balinda, Sheila N.; Tjørnehøj, Kirsten; Alexandersen, Søren

doi:10.1177/1040638711435807

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…Moreover, the 60–90% prevalence of antibodies against FMDV NSPs in unvaccinated herds from Bukedea, Isingiro, Kumi and Rakai districts indicates that the sampling took place after considerable spread of the infection within the herds, while the lower seroprevalence consistently observed in herds from Gomba (29%) and Sembabule (24%) districts, as well as in the non‐vaccinated herd in Kiruhura (45%), accords with sampling in an earlier phase of the outbreak (Sorensen et al., ). The very high prevalence of anti‐NSP antibodies in herd Kr.2 in Kiruhura district, which last had an outbreak in 1991 but where the cattle are vaccinated on a yearly basis, may be attributed to repeated use of non‐purified vaccines (Sutmoller et al., ; Ayebazibwe et al., ).…”

Section: Discussionmentioning

confidence: 99%

Challenges for Serology-Based Characterization of Foot-and-Mouth Disease Outbreaks in Endemic Areas; Identification of Two Separate Lineages of Serotype O FMDV in Uganda in 2011

Namatovu

Belsham

Ayebazibwe

et al. 2013

Transbound Emerg Dis

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Section: Discussionmentioning

confidence: 99%

Challenges for Serology-Based Characterization of Foot-and-Mouth Disease Outbreaks in Endemic Areas; Identification of Two Separate Lineages of Serotype O FMDV in Uganda in 2011

Namatovu

Belsham

Ayebazibwe

et al. 2013

Transbound Emerg Dis

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“…These tests can also differentiate infected from vaccinated animals (DIVA test) [33], however, in Eastern Africa, interpretation of NSP-test results is complicated by the frequent use of non-purified vaccines which elicit antibodies against NSPs, thereby limiting the DIVA application of these tests [20,34,35]. Furthermore, antibodies against NSPs do not appear until day 8–9 after infection [36], thus, to be useful, these tests should only be used for sera sampled in the late subacute and chronic phases.…”

Section: Discussionmentioning

confidence: 99%

Laboratory capacity for diagnosis of foot-and-mouth disease in Eastern Africa: implications for the progressive control pathway

et al. 2013

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BackgroundAccurate diagnosis is pertinent to any disease control programme. If Eastern Africa is to work towards control of foot-and-mouth disease (FMD) using the Progressive Control Pathway for FMD (PCP-FMD) as a tool, then the capacity of national reference laboratories (NRLs) mandated to diagnose FMD should match this task. This study assessed the laboratory capacity of 14 NRLs of the Eastern Africa Region Laboratory Network member countries using a semi-structured questionnaire and retrospective data from the World Reference Laboratory for FMD annual reports and Genbank® through National Centre for Biotechnology Information for the period 2006–2010.ResultsThe questionnaire response rate was 13/14 (93%). Twelve out of the 13 countries/regions had experienced at least one outbreak in the relevant five year period. Only two countries (Ethiopia and Kenya) had laboratories at biosecurity level 3 and only three (Ethiopia, Kenya and Sudan) had identified FMD virus serotypes for all reported outbreaks. Based on their own country/region assessment, 12/13 of these countries /regions were below stage 3 of the PCP-FMD. Quarantine (77%) and vaccination (54%) were the major FMD control strategies employed. The majority (12/13) of the NRLs used serological techniques to diagnose FMD, seven used antigen ELISA and three of these (25%) also used molecular techniques which were the tests most frequently requested from collaborating laboratories by the majority (69%) of the NRLs. Only 4/13 (31%) participated in proficiency testing for FMD. Four (31%) laboratories had no quality management systems (QMS) in place and where QMS existed it was still deficient, thus, none of the laboratories had achieved accreditation for FMD diagnosis.ConclusionsThis study indicates that FMD diagnostic capacity in Eastern Africa is still inadequate and largely depends on antigen and antibody ELISAs techniques undertaken by the NRLs. Hence, for the region to progress on the PCP-FMD, there is need to: implement regional control measures, improve the serological diagnostic test performance and laboratory capacity of the NRLs (including training of personnel as well as upgrading of equipment and methods, especially strengthening the molecular diagnostic capacity), and to establish a regional reference laboratory to enforce QMS and characterization of FMD virus containing samples.

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“…Among the NSPs of FMDV, 3ABC polyprotein is reported to be the most antigenic and the most reliable marker for DIVA. Various formats of ELISA based on the 3ABC polyprotein were developed, including the LPB-ELISA, SPC-ELISA, and direct/indirect sandwich ELISA, all of which demonstrated good sensitivity, specificity, and capability for DIVA in various animals (56,67,68,(116)(117)(118)(119)(120)(121)(122)(123)(124)(125). Enzyme-linked immunosorbent assays based on 3ABC have some added advantages over other NSPs including superior longevity of anti-3ABC antibody in infected animals compared to 2C, 3A, 3D, and Lb, and all infected cattle were shown to develop 3ABC-specific antibody at some points following the infection.…”

Section: Differentiation/discrimination Of Infected From Vaccinated Amentioning

confidence: 99%

Advances in the Diagnosis of Foot-and-Mouth Disease

et al. 2020

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Foot-and-mouth disease (FMD) is a devastating livestock disease caused by foot-and-mouth disease virus (FMDV). Outbreaks of this disease in a country always result in conspicuous economic losses to livestock industry and subsequently lead to serious socioeconomic damages due to the immediate imposition of trade embargo. Rapid and accurate diagnoses are imperative to control this infectious virus. In the current review, enzyme-linked immunosorbent assay (ELISA)-based methods used in FMD diagnosis are extensively reviewed, particularly the sandwich, liquid-phase blocking, and solid-phase competition ELISA. The differentiation of infected animals from vaccinated animals using ELISA-based methods is also highlighted, in which the role of 3ABC polyprotein as a marker is reviewed intensively. Recently, more studies are focusing on the molecular diagnostic methods, which detect the viral nucleic acids based on reverse transcription-polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (RT-LAMP). These methods are generally more sensitive because of their ability to amplify a minute amount of the viral nucleic acids. In this digital era, the RT-PCR and RT-LAMP are progressing toward the mobile versions, aiming for on-site FMDV diagnosis. Apart from RT-PCR and RT-LAMP, another diagnostic assay specifically designed for on-site diagnosis is the lateral flow immunochromatographic test strips. These test strips have some distinct advantages over other diagnostic methods, whereby the assay often does not require the aid of an external device, which greatly lowers the cost per test. In addition, the on-site diagnostic test can be easily performed by untrained personnel including farmers, and the results can be obtained in a few minutes. Lastly, the use of FMDV diagnostic assays for progressive control of the disease is also discussed critically.

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Application of the Ceditest® FMDV type O and FMDV-NS enzyme-linked immunosorbent assays for detection of antibodies against Foot-and-mouth disease virus in selected livestock and wildlife species in Uganda

Cited by 5 publications

References 19 publications

Challenges for Serology-Based Characterization of Foot-and-Mouth Disease Outbreaks in Endemic Areas; Identification of Two Separate Lineages of Serotype O FMDV in Uganda in 2011

Challenges for Serology-Based Characterization of Foot-and-Mouth Disease Outbreaks in Endemic Areas; Identification of Two Separate Lineages of Serotype O FMDV in Uganda in 2011

Laboratory capacity for diagnosis of foot-and-mouth disease in Eastern Africa: implications for the progressive control pathway

Advances in the Diagnosis of Foot-and-Mouth Disease

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