Challenges for Serology-Based Characterization of Foot-and-Mouth Disease Outbreaks in Endemic Areas; Identification of Two Separate Lineages of Serotype O FMDV in Uganda in 2011

Namatovu, Alice; Belsham, Graham J.; Ayebazibwe, C.; Dhikusooka, Moses Tefula; Wekesa, Sabenzia Nabalayo; Siegismund, Hans R.; Muwanika, Vincent B.; Tjørnehøj, Kirsten

doi:10.1111/tbed.12170

Cited by 16 publications

(21 citation statements)

References 42 publications

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“…The percentage identity within the lineage was 98.9 ± 0.4% at the nucleotide level, and 99.5 ± 0.5% at the amino acid level. The closet ancestral relationship of this lineage was found with a viral strain (U04/11) previously circulating in Bukedea district in eastern Uganda during 2011 (Namatovu, Belsham, et al, ). However, another ancestral relationship of lineage V can be appreciated with the viral strain O/UGA/3/2002 (Knowles & Samuel, ) previously recovered from Uganda during 2002.…”

Section: Resultssupporting

confidence: 56%

“…However, another ancestral relationship of lineage V can be appreciated with the viral strain O/UGA/3/2002 (Knowles & Samuel, ) previously recovered from Uganda during 2002. Interestingly, in both cases the closest ancestral relationships of viral strains O/UGA/3/2002 and U04/11(Namatovu, Belsham, et al, ) are strains previously circulating in Kenya during 2000 (K145/00) (Balinda, Sangula, et al, ) and 2010 (K2551/2010) suggesting that the circulation of these lineages in Uganda might be a product of introduction from elsewhere.…”

Section: Resultsmentioning

confidence: 95%

“…Based on the branch topology of our phylogenetic analysis, we could trace ancestral relationships of lineages II and IV to FMDV strains previously circulating in the central region of Uganda in 2005 (U12/05; Wakiso), 2006 (U18/06 and U/25/06; Mpigi) (Balinda, Sangula, et al, ), and 2011 (U/24/11; Sembabule, U/07/11; Rakai) (Namatovu, Belsham, et al, ).…”

Section: Resultsmentioning

confidence: 98%

“…The clinical signs in infected animals are characterized by fever, lameness and vesicular lesions of the feet, tongue, snout and teats. Although mortality is generally low, the disease causes economic losses such as declines in milk production and loss of access to commercial international livestock and livestock product markets (Knight‐Jones & Rushton, ; Namatovu, Belsham, et al, ; Thompson et al, ). FMD is caused by foot and mouth disease virus (FMDV), a single‐stranded positive‐sense RNA virus belonging to the genus Aphthovirus within the family Picornaviridae (Knowles & Samuel, ).…”

Section: Introductionmentioning

confidence: 99%

“…As such, imported FMD vaccines in Uganda have historically not contained FMD serotype A. In addition, factors like uncontrolled animal movements, limited surveillance causing delays in reporting of FMD outbreaks, the existence of wildlife reservoirs and low performance of available vaccines negatively impact control strategies implemented in this country (Balinda, Sangula, et al, ; Namatovu, Belsham, et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Serological and phylogenetic characterization of foot and mouth disease viruses from Uganda during cross‐sectional surveillance study in cattle between 2014 and 2017

Mwiine

Velazquez‐Salinas

Ahmed

et al. 2019

Transbound Emerg Dis

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Here, we report the results of a cross-sectional study designed to monitor the circulation and genetic diversity of foot and mouth disease virus (FMDV) in Uganda between 2014 and 2017. In this study, 13,614 sera and 2,068 oral-pharyngeal fluid samples were collected from cattle and analysed to determine FMDV seroprevalence, circulating serotypes and their phylogenetic relationships. Circulation of FMDV was evidenced by the detection of antibodies against non-structural proteins of FMDV or viral isolations in all districts sampled in Uganda. Sequence analysis revealed the presence of FMDV serotypes A, O, SAT 1 and SAT 2. FMDVs belonging to serotype O, isolated from 21 districts, were the most prevalent and were classified into six lineages within two East African topotypes, namely EA-1 and EA-2. Serotype A viruses belonging to the Africa G-I topotype were isolated from two districts. SAT 1 viruses grouped within topotypes I and IV and SAT 2 viruses within topotypes VII, IV and X were isolated from six and four districts respectively. Phylogenetic analysis of SAT 1 and SAT 2 sequences from cattle clustered with historical sequences from African buffalo, indicating possible interspecies transmission at the wildlife-livestock interface. In some cases, Uganda viruses also shared similarities to viral strains recovered from other regions in East Africa. This 3-year study period provides knowledge about the geographical distribution of FMDV serotypes isolated in Uganda and insights into the genetic diversity of the multiple serotypes circulating in the country. Knowledge of circulating FMDV viruses will assist in antigenic matching studies to devise improved FMDV control strategies with vaccination and vaccine strain selection for Uganda. K E Y W O R D Sbuffalo, EA-2, foot and mouth disease, molecular epidemiology, Uganda, vaccine

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Section: Resultssupporting

confidence: 56%

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 98%