Application of turbidity technique on peptide-lipid and drug-lipid interactions

Eker, Fatma; Durmus, Huseyin Okan; Akinoglu, Bülent G.; Severcan, Feride

doi:10.1016/s0022-2860(98)00690-5

Cited by 18 publications

(21 citation statements)

References 14 publications

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“…Besides a sharp main transition, a slight discontinuity could be discerned in methylene frequency dependence attributed to the so‐called pretransition L β —P β in the temperature range of 36°C to 38° C. Prior to the pretransition region, the ν s CH 2 vibration frequencies in both the control and the experiment are almost identical, which implies that the presence of compound 2 does not disturb the packing in the lipid gel phase. At the same time, the liquid crystalline phase frequency is higher relative to the control, which indicates more disordered state of the hydrophobic region of the bilayer.…”

Section: Resultssupporting

confidence: 88%

“…Then the ability of the synthesized imides to bind lipid bilayer was characterized by a number of physical methods. The degree of interaction of the compounds studied with the model lipid membrane was investigated with turbidimetry by the changes in the phase transition temperature of lipid vesicles in the presence of increasing concentrations of 1 to 4 . The constants of the binding of 1 to 4 to immobilized lipid bilayers were measured by the microgravimetry method, while the nature of interacting groups was determined by Fourier transform infrared (FTIR) spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

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Novel type of isoprenoid membrane anchors: an investigation of binding properties with dipalmitoylphosphatidylcholine vesicles

Faizullin

Dzyurkevich

Valiullina

et al. 2016

J of Physical Organic Chem

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In this work, we present a new type of amphiphilic membrane‐anchoring agents that can be easily obtained by the Diels‐Alder reaction between terpene myrcene and N‐substituted maleimides. The interaction between the compounds and small unilamellar dipalmitoylphosphatidylcholine vesicles was investigated using infrared spectroscopy, microgravimetry, and turbidimetry. The ability of the compounds to embed in the phospholipid membrane was shown to be strongly dependent on the charge of their polar group. The insertion of the compounds studied into the lipid bilayer did not lead to disruption of the dipalmitoylphosphatidylcholine vesicles up to the highest tested drug to lipid molar ratio of 0.5 to 0.6. Low lipid solubilization ability of the compounds as well as their rigid nonplanar structure makes them an interesting alternative to the common membrane‐anchoring structural motifs.

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Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Novel type of isoprenoid membrane anchors: an investigation of binding properties with dipalmitoylphosphatidylcholine vesicles

Faizullin

Dzyurkevich

Valiullina

et al. 2016

J of Physical Organic Chem

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“…The turbidity technique at visible range is a spectroscopic technique that provides valuable information about membrane phase transition temperatures and membrane order [42,43]. Lipid turbidity study has been previously utilised in membrane research [42,44,45].…”

Section: Resultsmentioning

confidence: 99%

Effect of chitosan coating on the characteristics of DPPC liposomes

Mady

Darwish

2010

Journal of Advanced Research

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Because it is both biocompatible and biodegradable, chitosan has been used to provide a protective capsule in new drug formulations. The present work reports on investigations into some of the physicochemical properties of chitosan-coated liposomes, including drug release rate, transmission electron microscopy (TEM), zeta potential and turbidity measurement. It was found that chitosan increases liposome stability during drug release. The coating of DPPC liposomes with a chitosan layer was confirmed by electron microscopy and the zeta potential of liposomes. The coating of liposomes by chitosan resulted in a marginal increase in the size of the liposomes, adding a layer of (92 ± 27.1 nm). The liposomal zeta potential was found to be increasingly positive as chitosan concentration increased from 0.1% to 0.3% (w/v), before stabilising at a relatively constant value. Turbidity studies revealed that the coating of DPPC liposomes with chitosan did not significantly modify the main phase transition temperature of DPPC at examined chitosan concentrations. The appropriate combination of liposomal and chitosan characteristics may produce liposomes with specific, prolonged and controlled release.

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“…It was clearly shown the turbidity of LF liposome decreased from 0.36 ± 0.03 to 0.2 ± 0.03 and 0.15 ± 0.03, and then maintained relative stable with further digestion, respectively. Severcan et al 39 found the UV absorbance values in turbidity of dipalmitoyl phosphatidylcholine (DPPC) multilamellar liposomes significantly decreased when the number of gauche conformers disordering of the lipids membrane increased. Our results showed the turbidity of LF liposomes decreased during incubation in SGF, which could be attributed to low pH environment.…”

Section: Changes In Turbidity Of Lf Liposomes During Infant Digestionmentioning

confidence: 99%

Structural characterization and biological fate of lactoferrin‐loaded liposomes during simulated infant digestion

Tian

Han

et al. 2019

J Sci Food Agric

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BACKGROUND Limited information is concerned on the structure changes of liposomal delivery system under infant conditions. Positively charged lactoferrin (LF)‐loaded liposomes, with the entrapment efficiency (EE) of 52.3 ± 6.3%, were prepared from soybean‐derived phospholipids using a thin‐layer dispersion method. The structure changes and digestibility of LF‐loaded liposomes under infant conditions, including simulated gastric fluid (SGF) and simulated small intestinal fluid (SIF), were characterized in terms of the average particle size, zeta potential, turbidity, fourier transform infrared, transmission electron microscopy, lipolysis and protein hydrolysis. RESULTS This study showed that the functional groups, favorable membrane structure and the EE of liposomes were slightly changed as a function of time when the liposome digested under SGF conditions. However, the intact bilayer structures were damaged and the EE of LF‐loaded liposomes decreased to 28.5% after digestion in infant SIF. CONCLUSION These results suggested that liposomal membrane could prevent the gastric degradation and the structure of liposomes was not completely destroyed with a low concentration of pancreatin and bile salts under infant conditions. Present study provided information on the insight into the characteristics of liposomes during infant gastrointestinal digestion, which was useful for the development of microcapsule systems in infant diet. © 2018 Society of Chemical Industry

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Application of turbidity technique on peptide-lipid and drug-lipid interactions

Cited by 18 publications

References 14 publications

Novel type of isoprenoid membrane anchors: an investigation of binding properties with dipalmitoylphosphatidylcholine vesicles

Novel type of isoprenoid membrane anchors: an investigation of binding properties with dipalmitoylphosphatidylcholine vesicles

Effect of chitosan coating on the characteristics of DPPC liposomes

Structural characterization and biological fate of lactoferrin‐loaded liposomes during simulated infant digestion

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