Application of Xerophytophysiology and Signal Transduction in Plant Production: Partial Root-Zone Drying in Potato Crops

Su, Feifei; Li, Yong; Liu, Shangwu; Liu, Zhenyu; Nie, Shengjun; Xu, Qicong; Qin, Feifei; Li, Fenglan; Lyu, Dianqiu; Xu, Hui-lian

doi:10.1007/s11540-019-09427-y

Cited by 11 publications

(8 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Potato ( Solanum tuberosum L.) is one of the four major food crops in the world and is commonly grown as a staple food crop in arid and semiarid regions with an annual average precipitation of less than 500 mm [ 1 ]. In these areas, the yield and quality of potato tubers are limited by many biotic and abiotic stresses, among which long-term or seasonal drought stress have detrimental effects on canopy growth as well as tuber yield and market value [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Deep-Rooting and Shallow-Rooting Potato (Solanum tuberosum L.) Genotypes under Drought Stress

Qin

Sun

Ali³

et al. 2022

Plants

View full text Add to dashboard Cite

The selection and breeding of deep rooting and drought-tolerant varieties has become a promising approach for improving the yield and adaptability of potato (Solanum tuberosum L.) in arid and semiarid areas. Therefore, the discovery of root-development-related genes and drought tolerance signaling pathways in potato is important. In this study, we used deep-rooting (C119) and shallow-rooting (C16) potato genotypes, with different levels of drought tolerance, to achieve this objective. Both genotypes were treated with 150 mM mannitol for 0 h (T0), 2 h (T2), 6 h (T6), 12 h (T12), and 24 h (T24), and their root tissues were subjected to comparative transcriptome analysis. A total of 531, 1571, 1247, and 3540 differentially expressed genes (DEGs) in C16 and 1531, 1108, 674, and 4850 DEGs in C119 were identified in T2 vs. T0, T6 vs. T2, T12 vs. T6, and T24 vs. T12 comparisons, respectively. Gene expression analysis indicated that a delay in the onset of drought-induced transcriptional changes in C16 compared with C119. Functional enrichment analysis revealed genotype-specific biological processes involved in drought stress tolerance. The metabolic pathways of plant hormone transduction and MAPK signaling were heavily involved in the resistance of C16 and C119 to drought, while abscisic acid (ABA), ethylene, and salicylic acid signal transduction pathways likely played more important roles in C119 stress responses. Furthermore, genes involved in root cell elongation and division showed differential expression between the two genotypes under drought stress. Overall, this study provides important information for the marker-assisted selection and breeding of drought-tolerant potato genotypes.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Deep-Rooting and Shallow-Rooting Potato (Solanum tuberosum L.) Genotypes under Drought Stress

Qin

Sun

Ali³

et al. 2022

Plants

View full text Add to dashboard Cite

show abstract

“…Since the lower osmotic potential would pull water from the apoplasm (cell walls) into the symplast (inside the membrane), water in the whole cell was re-compartmented, and the water fraction was more in the symplasm than in the apoplasm. Many studies have confirmed that this kind of water re-compartmenting is good for physiological and biochemical metabolisms in the symplasm [4][5][6][7][8][9][10][11][13][14]26,31,32,34,37]. Moreover, the osmotic potential at incipient plasmolysis (ΨIP) (cell walls just begin to separate from membrane) and leaf relative water content at plasmolysis (ζIP) were lower in sub-irrigation plots than in control plots.…”

Section: Discussionmentioning

confidence: 95%

“…The topic deals with stimulating treatments to plants under well-watered conditions instead of real drought conditions [1]. The study cases of xerophytophysiological applications include the exposure of sorghum mesocotyls [4] and peanut hypocotyls [5], partial root zone drying for crops of tomato [6] and potato [7], restricted irrigation for tomato [8,9], under-canopy blue light irradiation in tomato crop [10], and drying the cut trace of potato seed tubers [11].…”

Section: Introductionmentioning

confidence: 99%

Applications of Xerophytophysiology and Signal Transduction in Plant Production—Flower Qualities in Eustoma grandiflorum Were Improved by Sub-Irrigation

2023

View full text Add to dashboard Cite

Relatively mild xerophytic or hardening treatments can induce healthy development of plants. In the present study, as one of xerophytophysiological applications, sub-irrigation was applied to a flower plant of Eustoma grandiflorum to confirm whether the sub-irrigation improved flowering quality in addition to plant growth and physiology. As shown by the results, long-term sub-irrigation induced osmotic adjustment, with osmolyte concentration increasing 32.8 osmol m−3 (p ≤ 0.01), improved leaf photosynthetic activities, with more than 10% (p ≤ 0.05) increase in photosynthetic capacity, and promoted plant growth, with a shoot biomass increase by 27.5% (p ≤ 0.01) and a root increase by 50.5% (p ≤ 0.01). These improvements were attributed to turgor maintenance and cell water re-compartmentation into the symplasm, which were both the consequence of osmotic adjustment. The lower osmotic potential and lower relative leaf water potential at incipient plasmolysis suggested that plants in sub-irrigation plots might be more resistant to environmental stresses. Sub-irrigation also improved flower quality shown by increased anthocyanin concentration (16% up, p ≤ 0.01). Flower quality improvement might be attributed to up-regulation of the PAL gene, which could catalyze the synthesis of anthocyanins. PAL gene up-regulation might be associated with a concentration increase in salicylic acid (SA), which was suggested as a plant hormone for signaling. Sub-irrigation also affected the flower opening and closing oscillations with less changed opening size or oscillation amplitude. We adopted mathematical models and thoroughly analyzed dynamic changes in photosynthesis, plant growth, and flower opening oscillations. In conclusion, sub-irrigation was a feasible practice and could be used in E. grandiflorum culture to improve plant growth and flower opening quality.

show abstract

“…First, functional proteins, such as osmolytes, various proteases, aquaporins, molecular chaperones, and LEA protein enzymes, related to active oxygen scavenging, among others, are directly involved in various biochemical environmental stress response resistance processes, resulting in drought tolerance effects ( Battaglia et al., 2008 ; Ramanjulu and Bartels, 2022 ). Second, regulatory proteins act upstream of genes encoding functional proteins and mediate related signaling, for example, transcription factors and various kinases involved in signaling cascades ( Tõldsepp et al., 2018 ; Su et al., 2019 ). At the molecular level, plant drought stress transcriptional regulatory networks comprise two different regulatory pathways: abscisic acid (ABA)-dependent and ABA-independent ( Bulgakov et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Integration of mRNA and microRNA analysis reveals the molecular mechanisms underlying drought stress tolerance in maize (Zea mays L.)

Jiao

Ma²,

Wang³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Drought is among the most serious environmental issue globally, and seriously affects the development, growth, and yield of crops. Maize (Zea mays L.), an important crop and industrial raw material, is planted on a large scale worldwide and drought can lead to large-scale reductions in maize corn production; however, few studies have focused on the maize root system mechanisms underlying drought resistance. In this study, miRNA–mRNA analysis was performed to deeply analyze the molecular mechanisms involved in drought response in the maize root system under drought stress. Furthermore, preliminary investigation of the biological function of miR408a in the maize root system was also conducted. The morphological, physiological, and transcriptomic changes in the maize variety “M8186” at the seedling stage under 12% PEG 6000 drought treatment (0, 7, and 24 h) were analyzed. With prolonged drought stress, seedlings gradually withered, the root system grew significantly, and abscisic acid, brassinolide, lignin, glutathione, and trehalose content in the root system gradually increased. Furthermore, peroxidase activity increased, while gibberellic acid and jasmonic acid gradually decreased. Moreover, 32 differentially expressed miRNAs (DEMIRs), namely, 25 known miRNAs and 7 new miRNAs, and 3,765 differentially expressed mRNAs (DEMRs), were identified in maize root under drought stress by miRNA-seq and mRNA-seq analysis, respectively. Through combined miRNA–mRNA analysis, 16 miRNA–target gene pairs, comprising 9 DEMIRs and 15 DEMRs, were obtained. In addition, four metabolic pathways, namely, “plant hormone signal transduction”, “phenylpropane biosynthesis”, “glutathione metabolism”, and “starch and sucrose metabolism”, were predicted to have important roles in the response of the maize root system to drought. MiRNA and mRNA expression results were verified by real-time quantitative PCR. Finally, miR408a was selected for functional analysis and demonstrated to be a negative regulator of drought response, mainly through regulation of reactive oxygen species accumulation in the maize root system. This study helps to elaborate the regulatory response mechanisms of the maize root system under drought stress and predicts the biological functions of candidate miRNAs and mRNAs, providing strategies for subsequent mining for, and biological breeding to select for, drought-responsive genes in the maize root system.

show abstract

Application of Xerophytophysiology and Signal Transduction in Plant Production: Partial Root-Zone Drying in Potato Crops

Cited by 11 publications

References 40 publications

Comparative Transcriptome Analysis of Deep-Rooting and Shallow-Rooting Potato (Solanum tuberosum L.) Genotypes under Drought Stress

Comparative Transcriptome Analysis of Deep-Rooting and Shallow-Rooting Potato (Solanum tuberosum L.) Genotypes under Drought Stress

Applications of Xerophytophysiology and Signal Transduction in Plant Production—Flower Qualities in Eustoma grandiflorum Were Improved by Sub-Irrigation

Integration of mRNA and microRNA analysis reveals the molecular mechanisms underlying drought stress tolerance in maize (Zea mays L.)

Contact Info

Product

Resources

About