Applications in Food Analysis

Proof-of-Principle of rTLC, an Open-Source Software Developed for Image Evaluation and Multivariate Analysis of Planar Chromatograms

“…Though it is challenging, it is important to obtain reliable fingerprints that represent sound profiles of physiologically active compounds . Its simplicity, cost-effective operation, and the possibility of simultaneous analysis of up to 20 samples in parallel makes high-performance thin-layer chromatography (HPTLC) a technique of choice in herbal and food analysis. , For evaluation, the HPTLC fingerprint of a complex sample is visually compared to that of a certified reference sample or to marker compounds being characteristic for the respective sample. The main disadvantage of such a manual pattern recognition technique and its visual comparison is its subjectivity, and it highly depends on the analyst’s perception.…”