Applications of microcalorimetry in the antibacterial activity evaluation of various<i>Rhizoma coptidis</i>

Feng, Xue; Yan, Dan; Zhao, Kun; Luo, Jiaoyang; Ren, Yong; Kong, Weijun; Han, Ying; Xiao, Xiaohe

doi:10.3109/13880209.2010.523428

Cited by 27 publications

(23 citation statements)

References 17 publications

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“…The results in this study of the PCA of BFs showed that the bacteriostatic effects enhanced with the increase of the concentration, which was consistent with the preliminary experiment reported in the literature [29]. And the anti-bacterial activity of R. coptidis of different sources was found to be of the order CT>CC>CD.…”

Section: Discussionsupporting

confidence: 91%

“…To select the most important variables that could be used to evaluate the quality of the R. coptidis of different sources objectively [29], the PCA of the seven characteristic parameters k 1 , k 2 , p 1 , p 2 , t 1 , t 2 , and Q t (Table S1) at different concentrations and R. coptidis of different sources was performed using the SAS statistical software. On the basis of the eigenvalues being >1, two principal components accounting for 96.72% (the first was 80.63%, and the second was 16.09%) of the total variance were considered to be significant, and a score plot for PCA was shown in Figure 4.…”

Section: Bf Analysis Of R Coptidismentioning

confidence: 99%

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A quality evaluation strategy for Rhizoma coptidis from a variety of different sources using chromatographic fingerprinting combined with biological fingerprinting

Yan

Xiong

et al. 2013

Chin. Sci. Bull.

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Most of herbal medicines of different sources are used for the same treatment goal in clinic despite their tremendous differences of the quality. Because the clinical efficacy is closely related to the quality of medicines, the implementation of an effective quality control (QC) process is vital. Chemical methods based on content determination and chromatographic fingerprints (CFs) have been recommended as powerful tools. In spite of advances in QC, it is still difficult to determine that which of the chemical compositions can best reflect the correlation with the desired biological activity. Herein, we have focused on the biological activities of multi-source herbal medicines to establish an analytical method. By looking for correlations between the biological activities and their chemical compositions, the method can identify reasonable chemical indicators or chemical methods with biological activity information. In this study, high-performance liquid chromatography (HPLC) and microcalorimetry are combined as a useful method for evaluating the quality of Rhizoma coptidis from different sources. The results of biological fingerprints (BFs) showed that the antibacterial activity sequence of R. coptidis of different sources was Coptis teeta Wall. (CT)>Coptis chinensis Franch. (CC)>Coptis deltoidea C.Y. Cheng et Hsiao (CD). The "spectrum-activity" relationships between CFs and BFs were investigated using the canonical correlation analysis (CCA). Compared with the CCA result of CFs and BFs, the canonical correlation coefficient r 1 and r 2 were 0.9974 and 0.9752, respectively. It revealed that there are a close correlation between the spectrum-activity relationships and several of the major anti-bacterial components in the CFs, including jatrorrhizine, coptisine, palmatine, and berberine. Of course, R. coptidis was only a representative of multisource medical plants. The result of the current study will provide an effective reference for evaluating the quality of multisource herbal medicines.Rhizoma coptidis, multi-source medical plants, quality control, chromatographic fingerprint, biological fingerprint Citation:Li J X, Yan D, Ma L N, et al. A quality evaluation strategy for Rhizoma coptidis from a variety of different sources using chromatographic fingerprinting combined with biological fingerprinting.

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Section: Discussionsupporting

confidence: 91%

Section: Bf Analysis Of R Coptidismentioning

confidence: 99%

A quality evaluation strategy for Rhizoma coptidis from a variety of different sources using chromatographic fingerprinting combined with biological fingerprinting

Yan

Xiong

et al. 2013

Chin. Sci. Bull.

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“…chinensis has been used in China to treat inflammatory diseases and diabetes for centuries. In recent years, it has been reported that C. chinensis exhibits various pharmacobiological effects, such as antidiabetic [14,55], neuroprotective [13], antipyretic [29], antibacterial [11], immunomodulatory [37,43], antioxidant [22], and anticancer activities [48].…”

Section: Discussionmentioning

confidence: 99%

Antiangiogenic Activity of Coptis chinensis Franch. Water Extract in in vitro and ex vivo Angiogenesis Models

Kim¹,

Kim²,

Lee³

et al. 2017

Journal of Life Science

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Angiogenesis, the formation of new blood vessels, plays an important role in tumor growth and metastasis; therefore, it has become an important target in cancer therapy. Novel anticancer pharmaceutical products that have relatively few side effects or are non-cytotoxic must be developed, and such products may be obtained from traditional herbal medicines. Coptis chinensis Franch. is an herb used in traditional medicine for the treatment of inflammatory diseases and diabetes. However, potential antiangiogenic effects of C. chinensis water extract (CCFWE) have not yet been studied. The purpose of this study was to determine the antiangiogenic effect of CCFWE in order to evaluate its potential for an anticancer drug. We found that the treatment with CCFWE inhibited the major steps of the angiogenesis process, such as the endothelial cell proliferation, migration, invasion, and capillary-like tube formation in response to vascular endothelial growth factor (VEGF), and also resulted in the growth inhibition of new blood vessels in an ex vivo rat aortic ring assay. We also observed that CCFWE treatment arrested the cell cycle at the G0/G1 phase, preventing the G0/G1 to S phase cell cycle progression in response to VEGF. In addition, the treatment reduced the VEGF-induced activation of matrix metalloproteinases 2 and 9. Taken together, these findings indicate that CCFWE should be considered a potential anticancer therapy against pathological conditions where angiogenesis is stimulated during tumor development.

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“…It is able to measure the heat output from various biological processes, and the involved microorganisms include bacteria, fungi and mitochondria as well. Growth of these microorganisms results in an increase in heat flow over time which is documented by a continuous real-time electronic signal [25][26][27][28][29]. And in this study, heat flow curves and relative thermal parameters obtained by microcalorimetry were analyzed to compare the effects of three subjects.…”

Section: Introductionmentioning

confidence: 99%

Thermal activities of 6-gingerol, 8-gingerol and 6-shogaol on the potentiation of mitochondria thermogenesis based on microcalorimetry

Yang

Zhang

et al. 2016

J Therm Anal Calorim

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Zingiberis Rhizoma is a typical Chinese herb in 'hot' property. Nowadays, increasing attention has been aroused for its essential role in both health care and treatment of 'cold' syndrome. And research about characteristics of Zingiberis Rhizoma is able to provide an important reference for its deeper understand and successful clinic application. However, cognition level about the 'hot' property of Zingiberis Rhizoma is superficial, and mechanism behind the 'hot' presentation is still elusive and unclear. In this study, the 'hot' property of Zingiberis Rhizoma was investigated at a monomer level with assistance of microcalorimetry, an objective and sensitive method in evaluating biological activity. Three bioactive compounds including 6-gingerol, 8-gingerol and 6-shogaol were selected, and their thermal activities were explored with mitochondria as target. The power-time curves were recorded, and five thermal parameters (k, P max , T max , Q and P av ) were obtained. With PCA, P av was calculated to be the main parameter to measure the bioactivities of 6-gingerol, 8-gingerol and 6-shogaol. Finally, the bioactivities of three subjects were compared with sequence to be 6-gingerol [ 8-gingerol [ 6-shogaol. Generally, our study is promising to offer a reference to the research about characteristics of Zingiberis Rhizoma or other herbs and is of great interest for clinic practice.

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Applications of microcalorimetry in the antibacterial activity evaluation of variousRhizoma coptidis

Cited by 27 publications

References 17 publications

A quality evaluation strategy for Rhizoma coptidis from a variety of different sources using chromatographic fingerprinting combined with biological fingerprinting

A quality evaluation strategy for Rhizoma coptidis from a variety of different sources using chromatographic fingerprinting combined with biological fingerprinting

Antiangiogenic Activity of Coptis chinensis Franch. Water Extract in in vitro and ex vivo Angiogenesis Models

Thermal activities of 6-gingerol, 8-gingerol and 6-shogaol on the potentiation of mitochondria thermogenesis based on microcalorimetry

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