Applications of SELDI‐MS technology in oncology

Abstract

Considerable interest, speculation and controversy have been generated utilising surface-enhanced laser desorption/ionization in conjunction with mass spectrometry (SELDI-MS) for the diagnosis, prognosis and therapeutic monitoring of cancer and offers an attractive approach to cancer biomarker discovery from tissues and biological fluids. This technology utilises a combination of mass spectrometry and chromatography to facilitate protein profiling of complex biological mixtures. Compared to some other more traditional proteomic platforms, such as 2D polyacrylamide gel electrophoresis, it has a high-throughput capability and can resolve low-mass proteins. However, a considerable number of challenging issues related to the design of studies, including reproducibility, sensitivity, specificity, variation in sample collection, processing and storage, have been reported as problematic with this technology; albeit some of these concerns could perhaps also be lauded against other proteomic approaches that have attempted to address complex protein mixtures, such as plasma. Applications, successes and limitations of SELDI-MS in both clinical and basic science arenas will be reviewed in this article.

Biomarker Characterization by MALDI–TOF/MS

“…Various biofluids including plasma, serum, saliva, urine, tears, amniotic fluid, cerebrospinal liquid, bronchoalveolar fluid, and nipple aspirate fluid were analyzed by SELDI-TOF/MS to identify biomarkers for disease diagnosis . A large number of studies have been reported on the applications of SELDI-TOF/MS in protein profiling of biofluid samples for disease, particularly cancers such as breast, prostate, hepatocellular, ovarian, nasopharyngeal, testicular, colon, lung, pancreas, choriocarcinoma, osteoarthritis, remote ischemic preconditioning, and juvenile idiopathic arthritis [284][285][286][287][288][289][290][291][292][293][294]. The results of these studies showed a potential for large-scale disease screening using SELDI-TOF/MS.…”

Proteomic Effects of the Coagulation Proteinase Thrombin on LX-2 Hepatic Stellate Cells

“…Surface-enhanced laser desorption/ionization mass spectrometry (SELDI-MS) is a proteomic analysis (10) that has been demonstrated to be a very suitable approach to evaluate complex variations on protein level in tissue and cells (11,12).…”

Identification of a β-Casein-Like Peptide in Breast Nipple Aspirate Fluid That is Associated with Breast Cancer

“…The technology utilizes a combination of MS and chips with affinity to specific protein types to facilitate protein profiling of complex biological mixtures. It has higher throughput capability than 2D polyacrylamide gel electrophoresis [3]. Preliminary studies using SELDI-TOF MS assessment of body fluids to detect breast cancer have focused primarily on serum and plasma.…”