Approaches for coupling solid-phase microextraction to nanospray

Development of Coated Blade Spray Ionization Mass Spectrometry for the Quantitation of Target Analytes Present in Complex Matrices

“…As recently reviewed, [5] different geometries of SPME have been coupled to direct analysis in real time (DART), desorption electrospray ionization (DESI), surface-enhanced laser desorption-ionization (SELDI), and matrix-assisted laser desorptionionization (MALDI) in a broad range of applications. [6][7][8][9][10][11][12][13] We report herein the development of a novel SPME configuration that allows its use, without further modifications, as an ambient ionization method for mass spectrometry.…”

SPME as a promising tool in translational medicine and drug discovery: From bench to bedside

“…This idea was further explored, and recent results showed that an appropriately designed and developed SPME device can give limits of detection in the low pg mL −1 level in biological matrices, with a total analysis time (from sample preparation to result) of less than 3 min per sample for SPME-DART (Direct Analysis in Real Time) [91] and Coated Blade Spray (CBS) [92]. While direct coupling of SPME fibers to MS through the use of nanoelectrospray technology was first proposed in 2005 by Walles et al [93], recent modifications of the interface followed by method optimization have enabled for stable desorption conditions to be achieved with similar detection as previous approaches [94]. The given examples of direct SPME-MS couplings with both lab-made and commercially available devices show the enormous potential of the method for point-of-care and on-site diagnostics.…”

Development, Optimization and Applications of Thin Film Solid Phase Microextraction (TF-SPME) Devices for Thermal Desorption: A Comprehensive Review

“…This desorption mode is a necessity for liquid-phase separations; most commonly used for non-volatiles, thermally labile compounds and biomolecules [24]. Previous studies have shown promise for the direct coupling of SPME to nanoelectrospray ionization [25,26], the nanospray solvent effectively desorbing the SPME device. More recently, nanomaterial-based TF-SPME devices have been simultaneously desorbed and analyzed by total reflection X-ray fluorescence spectrometry (TXRF) [27,28] which can be applied to both the analysis of organic compounds and metals.…”