Aptamer based biosensors for detection of Staphylococcus aureus

Novel impedimetric aptasensor for label-free detection of Escherichia coli O157:H7

“…Moreover, in the course of their chemical synthesis it is easy to modify the aptamer sequence introducing specific active terminal groups that help to immobilize effectively these molecules on different transducers, thus forming biosensors, commonly named as aptasensors (Ravalli et al 2016). In recent years a large number of publications have been focused on the development of aptamer-based biosensor for detection of pathogenic bacteria: Salmonella (Sheikhzadeh et al 2016), Staphylococcus aureus (Shahdordizadeh et al 2017), Listeria [(Sidhu et al 2016), and also E.…”

Novel diagnostics for point-of-care bacterial detection and identification

“…15,34 Upon binding to their target analyte, aptamers fold into specic threedimensional structures with many surface interactions for strong bonding, 34 typically with a dissociation constant in the nano-or pico-molar level. 35,36 Aptamers are chemically synthesized in vitro by a process known as Systematic Evolution of Ligands by Exponential enrichment (SELEX), which involves three key steps: (1) the incubation of an oligonucleotide sequence library with the target analyte to assess which structures bind; (2) the elution of unbound oligonucleotides, separating them from those bound to the analyte; and (3) amplication of the remaining oligonucleotide sequences by PCR. These three steps can be repeated up to 20 times until a small collection of sequences with high affinity for the target are identied.…”

Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system

“…To resolve this issue, three aptamer-CDs/GO systems using aptamer candidates of PA1, PA2, and PA4 were generated to implement the uorescence assay. [19][20][21][22]…”