Aptamer Grafting onto (on) and into (in) Pegylated Gold Nanoparticles: Physicochemical Characterization and In vitro Cytotoxicity Investigation in Renal Cells

Arib, Célia; Milano, Serena; Gerbino, Andrea; Spadavecchia, Jolanda

doi:10.4172/2157-7439.1000520

Cited by 4 publications

(10 citation statements)

References 64 publications

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“…44 Recently, we have functionalized and characterized a renal aptamer on pegylated gold nanoparticles (PEG-AuNPs) by two chemical conjugations. 24 Previously, Dam et al 45 showed that loading of aptamer AS1411 onto AuNPs could be regulated through the charge of the ligands and pH. In our case, the loading of APT onto PEG-AuNPs was confirmed by the absorption peak at 260 nm from APT (Figure S2, Supporting Information).…”

Section: Aptamer Loading Onto Pegylated Gold Nanoparticles (Apt On Pe...supporting

confidence: 64%

“…This value confirms the aptamer length. 24 Moreover, ζ-potential measurements confirm the stability of PEG-AuNPs and APT PEG-AuNPs at physiological pH (ζ-potential = −28 ± 1 MV, −30 mV for APT ON PEG-AuNPs and −33 ± 1 mV for APT IN PEG-AuNPs) (Table 1). This stability was improved by the presence of the PEG coating.…”

Section: Aptamer Bioconjugationmentioning

confidence: 72%

“…In the first case, APT was coated on the surface of PEG-AuNPs via amide bonding between the carboxylic (COOH) groups on the surface of the PEG-AuNPs and the amino (NH 2 ) groups of the aptamer (APT ON PEG-AuNPs) through previous activation of EDC/NHS by carbodiimide chemistry 23 (Scheme 1 panel A). In the second case, APT takes part actively in the nucleation and growth of PEG-AuNPs via a chelation reaction (APT IN PEG-AuNPs), as previously described for other types of aptamers and biomolecules 24 (Scheme 1 panel B).…”

Section: Resultsmentioning

confidence: 99%

“…23,24 The second strategy consists of the complexation between the SOD 4 aptamer (50 μL of APT 10 μM; PBS pH 9) (APT), gold salt (HAuCl 4 •3H 2 O) (5 mL, 2.6 × 10 −4 M), and 50 μL of PEG diacid (PEG) (1 mM) through a chelation reaction, to form APT IN PEG-AuNPs as described above. 24 4.7. Aptamer Loading Efficiency.…”

Section: Discussionmentioning

confidence: 99%

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Aptamer–Gold(III) Complex Nanoparticles: A New Way to Detect Cu, Zn SOD Glycoprotein

et al. 2020

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Aptamers are small biomolecules composed of 20–100 nucleotides that recognize target molecules in three-dimensional structures. These natural targeting molecules have attracted interest in the biomedical field as biomarkers for cancer diagnostics. In this study, we investigated the interaction of a characteristic aptamer with its target protein, Cu, Zn superoxide dismutase (SOD 4), on a gold nanoparticle (AuNP) surface under experimental conditions. For this purpose, we applied two protocols to coat SOD 4 aptamer (APT) on the nanoparticle surface: carbodiimide chemistry (EDC/NHS) (Method ON) and a complexation methodology (Method IN). The nano-aptamer’s interactions with SOD 4 were detected by UV–vis absorption and Raman spectroscopy in a range of protein concentrations (from 1 μM to 50 nM). We believe that the interaction is heavily dependent on the nature of the biomarker (SOD 4) and also on the steric arrangement of the aptamer on the gold nanoparticle surface. The lowest detectable concentration (limit of detection, LOD) was about 2 nM for APT IN PEG-AuNPs and 8 nM for APT ON PEG-AuNPs. For the first time, we demonstrated a very sensitive detection of SOD 4 in the nanomolar concentration range with new ways of biosensor synthesis (APT IN and ON), providing a very strong tool to understand the effect of aptamer conformation to detect SOD 4.

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Section: Aptamer Loading Onto Pegylated Gold Nanoparticles (Apt On Pe...supporting

confidence: 64%

Section: Aptamer Bioconjugationmentioning

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Aptamer–Gold(III) Complex Nanoparticles: A New Way to Detect Cu, Zn SOD Glycoprotein

et al. 2020

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“…In the latest years, Spadavecchia et al have in-depth investigated the synthesis of hybrid nanoparticles based on drugs and/or biomolecules-gold-complex by an experimental approach named "Method IN", in which the drugs or biomolecules interact actively with gold salt (HAuCl 4 ) by chelation bonding. A biocompatible polymer molecule (PEG diacid) acts as surfactant, in order to form hybrid gold nanoparticles upon reduction by NaBH 4 [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Galectin-1 protein modified gold (III)-PEGylated complex-nanoparticles: Proof of concept of alternative probe in colorimetric glucose detection

Liu

Aouidat

Sacco

et al. 2020

Colloids and Surfaces B: Biointerfaces

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Galectins (Gal) are a family of dimeric lectins, composed by two galactoside-binding sites implicated in the regulation of cancer progression and immune responses. In this study, we report for the first time the synthesis and the physical-chemical characterization of galectin-1-complex-gold COOHterminated polyethlenglicole (PEG)-coated NPs (Gal-1 IN PEG-AuNPs) and their ability to recognize glucose in an aqueous solution with a concentration varying from 10 mM to 100 pM. The chemical protocol consistsof three steps: (i) complexation between galectin-1Gal-1 and tetrachloroauric acid (HAuCl 4) to form gold-protein grains; (ii) staking process of COOH-terminated polyethlenglicole molecules (PEG) onto Gal-1-Au complex and (iii) reduction of hybrid metal ions to obtain a colloidal stable solution. During the complexation, the spectral signatures related to the Gal-1 orientation on the gold surface have been found to change due to its protonation state. The effective glucose monitoring was detected by UV-Vis, Raman spectroscopy and Transmission Electron Microscopy (TEM). Overall, we observed that the interaction is strongly dependent on the Gal-1 conformation at the surface of gold nanoparticles.

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Vortex Nanodiscs Functionalization to Overcome Macrophage Recognition for Efficient Theragnosis Applications

Magalhães,

Nunes,

Costa Lima

et al. 2024

Adv Eng Mater

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Biological barriers prevent nanotherapeutics successful accumulation at target cells, limiting diagnosis and treatment responses. Magnetic nanodiscs with a spin‐vortex ground state have shown great promise for magnetomechanical cancer cells annihilation and for neuronal stimulation, requiring very low concentrations for an effective result. However, the biological barriers that these particles encounter upon intravenous administration remain a challenge. Herein, the synthesis of biocompatible multilayered Au/Fe/Au nanodiscs with a spin‐vortex ground state and their inert surface modification is reported. Two different surface modifications with two distinct polyethylene glycol (PEG) molecules are performed, which successfully reduce macrophage uptake, while maintaining the nanodiscs’ biocompatibility. By effectively preventing nanodisc uptake, innovative design features can be rationally incorporated to create a new generation of specific nanotherapeutics by modifying the PEG surface with specific targeting molecules.

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Aptamer Grafting onto (on) and into (in) Pegylated Gold Nanoparticles: Physicochemical Characterization and In vitro Cytotoxicity Investigation in Renal Cells

Cited by 4 publications

References 64 publications

Aptamer–Gold(III) Complex Nanoparticles: A New Way to Detect Cu, Zn SOD Glycoprotein

Aptamer–Gold(III) Complex Nanoparticles: A New Way to Detect Cu, Zn SOD Glycoprotein

Galectin-1 protein modified gold (III)-PEGylated complex-nanoparticles: Proof of concept of alternative probe in colorimetric glucose detection

Vortex Nanodiscs Functionalization to Overcome Macrophage Recognition for Efficient Theragnosis Applications

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