Aptamer Self-Assembly-Functionalized Nanochannels for Sensitive and Precise Detection of Chloramphenicol

Abstract

Sensitive and precise determination of chloramphenicol (CAP) is of great significance for human health due to its high risk in trace amounts. Solid-state artificial nanochannels are expected to be highly promising sensing devices owing to singlemolecule sensitivity, target-specific selectivity, and portability. Herein, we report an aptamer self-assembly-functionalized artificial nanochannel-based sensor for highly sensitive and precise determination of CAP. Aptamer self-assembly (AAs) served as the specific recognition component and were in situ grown on the surface of stable anodic aluminum oxide (AAO) nanochannels to develop an AAs@AAO nanochannel-based sensor. Selective interaction with CAP led to the disassembly of AAs and sensitive current change of AAs@AAO nanochannels, allowing sensitive and precise sensing of CAP in complex food samples. The developed AAs@AAO nanochannel-based sensor showed a wide linear range from 0.32 to 1600 pg. mL −1 , low limit of detection (LOD) of 0.1 pg. mL −1 , high precision with relative standard deviation of 2.9%, and quantitative recoveries of 93.4−102.2% for CAP in milk, milk powder, and honey samples. This work proposes a versatile nanochannel-based platform for facile, sensitive, and precise sensing of hazardous residues in food samples.

Integrating Ordered Two-Dimensional Covalent Organic Frameworks to Solid-State Nanofluidic Channels for Ultrafast and Sensitive Detection of Mercury

“…Analytical samples were digested on an ETHOS UP microwave digestion instrument (Milestone, Italy). A laboratory-built system as described in our previous work 19 was used for the detection of Hg(II).…”

“…Typically, the amino-functionalized AAO (AAO-NH 2 ) was prepared according to our previous work. 19 For the in situ growth of JNU-3 on AAO-NH 2 , AAO-NH 2 was immersed in a mixture containing DMAC (0.8 mL), o-DCB (0.2 mL), 3 M TFA (0.1 mL), Tp (0.075 mmol), and Pa-S (0.112 mmol) at room temperature (RT) for 3 days, then rinsed with DMF and MeOH, and vacuum-dried at RT to obtain JNU-3@AAO.…”

“…Specific recognition moiety-functionalized solid-state nanofluidic channels have been shown to be selective and sensitive sensors for various analytes. − Steady-state current change with chemical composition, surface charge, wettability, and the diameter of the nanofluidic channel caused by even a single molecule can be efficaciously recorded, endowing nanofluidic sensors with sensitivity down to aM levels. ,− The introduction of various specific recognition moieties including aptamer, phenylboronic acid, antibodies, and functional metal–organic frameworks brings the nanofluidic channel target-specific selectivity. , However, the time-consuming interaction of the recognition moieties and the targets is a great limitation for the application of nanofluidic channel-based sensors in rapid detection. ,− Thus, promoting the kinetics for the interaction between the analyte and the recognition moiety on the nanofluidic channels is the key to overcome the limitation.…”

Recent Progress in the Construction of Artificial Nanochannels for Biosensing

“…Super‐sandwich functionalization strategy on AAO nanochennels was recently reported by Yan's group (Figure 4). [4g] This method firstly modified the AAO nanochannels with amino group via condensation reaction between the hydroxyl group in AAO and the aldehyde groups on the aptamers. The AAO nanochannels were further functionalized in a sandwich way by alternatively hybridizing aptamer and the corresponding linker.…”

Triple-Helix Molecular Switch Triggered Cleavage Effect of DNAzyme for Ultrasensitive Electrochemical Detection of Chloramphenicol

“…As the primary mass-produced antibiotic, chloramphenicol (CAP) is broadly adopted as a veterinary drug in the aquaculture industry . However, improper use or abuse of CAP can cause traces of CAP residues in animal-derived foods, which can lead to serious side effects in humans, including leukemia, kidney damage, and aplastic anemia. − Consequently, China and the European Union have prohibited the use of CAP in the animal-breeding industry. , Even so, the illegal abuse of CAP still happens sometimes because it is cost-effective.…”