Aquaporins mediate the chemoresistance of human melanoma cells to arsenite

Gao, Lin; Gao, Yanhui; Li, Xiaobo; Howell, Paul; Kumar, Rajeev; Su, Xiulan; Vlassov, Alexander V.; Piazza, Gary A.; Riker, Adam I.; Sun, Dianjun; Xi, Yaguang

doi:10.1016/j.molonc.2011.11.001

Cited by 40 publications

(32 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whether AQP3 participates in chemoresistance of GC remains unclear. Gao et al 14 have shown that AQP3 and AQP9 overexpression inhibits the therapeutic effect of arsenite in melanoma. 14 In this study, we showed that AQP3 overexpression is associated with cDDP resistance in GC cells.…”

Section: Discussionmentioning

confidence: 99%

“…Gao et al 14 have shown that AQP3 and AQP9 overexpression inhibits the therapeutic effect of arsenite in melanoma. 14 In this study, we showed that AQP3 overexpression is associated with cDDP resistance in GC cells. The artificial upregulation of AQP3 in AGS cells resulted in resistance to cDDP, whereas AQP3 silencing in MGC803 and SGC7901 cells enhanced the cytotoxicity of cDDP.…”

Section: Discussionmentioning

confidence: 99%

“…Although AQP3 overexpression has been demonstrated to contribute to chemoresistance in melanoma to arsenite, 14 little is known about its role in chemosensitivity of GC to cytotoxic agents. In this study, we showed for the first time that AQP3 mediates chemoresistance in gastric cancer cells to cisplatin, enhances autophagy of gastric cancer cells and the lysosome inhibitor chloroquine reverses the chemoresistance induced by AQP3.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Aquaporin 3 facilitates chemoresistance in gastric cancer cells to cisplatin via autophagy

Dong

Wang

Zhou

et al. 2016

Cell Death Discovery

View full text Add to dashboard Cite

Cisplatin (cDDP) remains one of the first-line chemotherapeutic agents for gastric cancer (GC) treatment, and resistance to cDDP is the major limitation in its clinical application. Mechanisms of cDDP resistance have been shown to be varied and complicated. Aquaporin 3 (AQP3) has been demonstrated to be overexpressed in GC tissues and is thought to be involved in GC carcinogenesis and progression. However, the role of AQP3 in chemosensitivity of GC to cytotoxic agents remains unknown. In this study, we show that AQP3 overexpression induced resistance to cDDP in AGS cells (P<0.05), and AQP3 knockdown increased the chemosensitivity in MGC803 and SGC7901 cells (P<0.05). Moreover, cDDP treatment enhanced AQP3 expression in MGC803, SGC7901 and AGS cells. AQP3 overexpression promoted the conversion of LC3-I to LC3-II in AGS cells, whereas AQP3 knockdown inhibited this conversion in MGC803 and SGC7901 cells. AQP3 upregulation increased Atg5 and Beclin-1 expression, and inhibited P62 expression in AGS cells, whereas AQP3 knockdown showed the opposite results in MGC803 and SGC7901 cells. Chloroquine (CQ), an autophagy inhibitor, enhanced the cytotoxicity of cDDP in GC cells, and CQ reversed the chemoresistance to cDDP caused by AQP3 overexpression in GC cells. Together, our data demonstrate that AQP3 facilitates cisplatin resistance in gastric cancer cells via autophagy, and suggest that the development of AQP3-based tumor therapeutics could play a key role in future GC treatment strategies.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Aquaporin 3 facilitates chemoresistance in gastric cancer cells to cisplatin via autophagy

Dong

Wang

Zhou

et al. 2016

Cell Death Discovery

View full text Add to dashboard Cite

show abstract

“…88 The over-expression of AQP3 and AQP9 in human melanoma cells revealed that both AQPs significantly increased the chemoresistance of these cells to apoptosis induced by arsenite, through a mechanism that involved downregulation of p53 and upregulation of Bcl-2 and XIAP. 134 This implicates AQPs in melanoma progression and resistance to apoptosis. Similarly, in primary squamous cell carcinoma, which shows high levels of AQP3, treatment with CuSO 4 , a pan-AQP inhibitor, caused apoptotic cell death in a concentration-dependent manner, 16 and in ovarian cancer cell line, epigallocatechin gallate, a potential anti-cancer drug, showed strong anti-proliferative effects and apoptosis induction accompanied by a downregulation of AQP5 expression.…”

Section: Apoptosis and Aqpsmentioning

confidence: 96%

Role of aquaporins in cell proliferation: What else beyond water permeability?

2016

View full text Add to dashboard Cite

In addition to the extensive data demonstrating the importance of mammalian AQPs for the movement of water and some small solutes across the cell membrane, there is now a growing body of evidence indicating the involvement of these proteins in numerous cellular processes seemingly unrelated, at least some of them in a direct way, to their canonical function of water permeation. Here, we have presented a broad range of evidence demonstrating that these proteins have a role in cell proliferation by various different mechanisms, namely, by allowing fast cell volume regulation during cell division; by affecting progression of cell cycle and helping maintain the balance between proliferation and apoptosis, and by crosstalk with other cell membrane proteins or transcription factors that, in turn, modulate progression of the cell cycle or regulate biosynthesis pathways of cell structural components. In the end, however, after discussing all these data that strongly support a role for AQPs in the cell proliferation process, it remains impossible to conclude that all these other functions attributed to AQPs occur completely independently of their water permeability, and there is a need for new experiments designed specifically to address this interesting issue.

show abstract

“…Duffy) system were studied using routine genetic manipulation techniques [53]. The implication of aquaporin-3 that bears determinant components for the GIL blood group system in mechanisms underlying resistance to chemotherapy drugs was studied by lentiviral vector transfer of the cDNA coding for this protein into cell lines derived from melanoma [54]. The impact of antigenic determinants carried by the ABO system on transplant organs was addressed using stimulation or inhibition of expression in animal models [55,56].…”

Section: A Strategy For Characterization Of Blood Group Systemsmentioning

confidence: 99%

Silencing and overexpression of human blood group antigens in transfusion: Paving the way for the next steps

Bagnis

2015

Blood Reviews

View full text Add to dashboard Cite

Aquaporins mediate the chemoresistance of human melanoma cells to arsenite

Cited by 40 publications

References 33 publications

Aquaporin 3 facilitates chemoresistance in gastric cancer cells to cisplatin via autophagy

Aquaporin 3 facilitates chemoresistance in gastric cancer cells to cisplatin via autophagy

Role of aquaporins in cell proliferation: What else beyond water permeability?

Silencing and overexpression of human blood group antigens in transfusion: Paving the way for the next steps

Contact Info

Product

Resources

About