Aquareoviruses

Lupiani, Blanca; Subramanian, Kothandaraman; Samal, Siba K.

doi:10.1016/0959-8030(95)00006-2

Cited by 66 publications

(38 citation statements)

References 51 publications

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“…CPE did not occur at 20°C and progressed slowly at 15°C affecting approximately 25% of the monolayer after 21 d. The CPE occurred in the cells both with and without PEG pre-treatment, although more cells were affected if they had been pretreated with PEG. After a further passage CPE occurred more rapidly, and affected approximately 50% of PEG pre-treated cells and 25% of nontreated cells after incubation for 9 d. The CPE exhibited as syncytia which gradually increased in size and eventually detached from the rest of the cell monolayer on the plastic substrate; the CPE was typical of that described for aquareoviruses (Lupiani et al 1995) (Fig. 3).…”

Section: Samplesmentioning

confidence: 69%

Four years of monitoring for viral haemorrhagic septicaemia virus in marine waters around the United Kingdom

Dixon

Avery²,

Chambers³

et al. 2003

Dis. Aquat. Org.

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Between 1995 and 1998, marine fish from around the coast of the UK were collected and samples analysed for viral haemorrhagic septicaemia virus (VHSV) using cell culture isolation methods. In 1997 and 1998 the samples were also analysed for VHSV by reverse transcription PCR (RT-PCR). A total of 1867 fish of 11 species were tested, but VHSV was isolated on only 1 occasion, from herring Clupea harengus, in 1996 . However, despite VHSV not being isolated in 1997 and 1998, in both years samples of herring from the west and south coasts of England produced positive signals in the RT-PCR, and in 1997 cod from the east coast of England also produced positive signals in the RT-PCR. These results are believed to be true indications of the presence of VHSV nucleic acid in the fish. In 1997, birnaviruses from Serogroup B1 were isolated from herring (a previously unrecorded host for the virus) and cod Gadus morhua, and a birnavirus from Serogroup A2 was also isolated from cod. In 1998, an aquareovirus was isolated from haddock Melanogrammus aeglefinus, a previously unrecorded host for the virus.

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Section: Samplesmentioning

confidence: 69%

Four years of monitoring for viral haemorrhagic septicaemia virus in marine waters around the United Kingdom

Dixon

Avery²,

Chambers³

et al. 2003

Dis. Aquat. Org.

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“…GCRV is highly contagious and can cause high mortalities (50-95%) not only to its host, grass carp (Ctenopharyngodon idellus), but also to black carp (Mylopharyngodon piceus), topmouth gudgeon (Pseudorasbora parva) (Li et al, 1997) and rare minnow (Gobiocypris rarus) (Wang et al, 1994). Other aquareovirus strains such as Chum salmon reovirus from Japan, Striped bass reovirus from USA, Smelt reovirus (SRV) from Canada, Angelfish reovirus (AFRV), all from the species Aquareovirus A, have also been reported to cause mortalities and losses to fish farmers (Lupiani et al, 1995). Therefore, the diseases caused by these pathogenic strains of aquareovirus may pose a major threat to aquaculture industries if no measures are taken to prevent its spread.…”

Section: Introductionmentioning

confidence: 99%

“…The genome of the members from this genus is composed of 11 segments of double-stranded (ds) RNA. Aquareoviruses have been isolated from a wide range of aquatic animals including shellfish, crustaceans, and fish worldwide and are among the more frequently isolated groups of finfish viruses (Hetrick and Hedrick, 1993;Lupiani et al, 1995). Many of these viral isolates were obtained from apparently healthy fish, which were undergoing routine examination.…”

Section: Introductionmentioning

confidence: 99%

Development of a rapid, sensitive and specific diagnostic assay for fish Aquareovirus based on RT-PCR

Seng

Qin

Lam

et al. 2004

Journal of Virological Methods

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“…The family Reoviridae presently has 9 genera, one of which is the new genus Aquareovirus that includes the reoviruses isolated from fish (Murphy et al 1995). Since the first isolation of a reovirus from golden shiners Notemigonus crysoleucas in 1977 in the USA, more than 30 aquareoviruses have so far been isolated from fish and shellfish worldwide (Lupiani et al 1996). The present study was aimed at characterisation of a reovirus-like agent recovered from the above-described characteristically EUS-infected snakehead fish.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of a new reovirus isolated from epizootic ulcerative syndrome infected snakehead fish

Mr²,

Rh³

et al. 2001

Dis. Aquat. Org.

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Epizootic ulcerative syndrome (EUS) has been infecting a wide range of fishes in the South and Southeast Asia for the last 2 decades. One reovirus-like agent (snakehead reovirus, SKRV), isolated from an EUS-infected snakehead fish and investigated in the present study, is the only reovirus so far isolated from an EUS-infected fish. SKRV was characterised by the presence of a double-stranded RNA genome with icosahedral symmetry and double capsid. The virus had an average size of 71 nm, a buoyant density of 1.36 g ml -1 in CsCl and lacked a lipid-containing envelope. Apart from the above, the presence of a segmented genome and structural proteins falling into 3 specific size classes confirmed that the virus belongs to the family Reoviridae. SKRV differed from aquareoviruses by the lack of a cytopathic effect (CPE) with syncitium formation and in the segmentation pattern of RNA genome. The resistance to pH (3.0 to 9.0) and heat treatment and inability to multiply in mammalian cell lines and haemagglutinate human 'O' red blood cells (RBCs) differentiated SKRV from the rest of the similar genera in the family Reoviridae. Serological comparison indicated the antigenic distinctness of the isolate from selected American and European aquareoviruses. SKRV grew well in SSN-1 and SSN-3 cells at 25 to 30°C but not in the most common Aquareovirus susceptible coldwater fish cell line -CHSE-214.

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Aquareoviruses

Cited by 66 publications

References 51 publications

Four years of monitoring for viral haemorrhagic septicaemia virus in marine waters around the United Kingdom

Four years of monitoring for viral haemorrhagic septicaemia virus in marine waters around the United Kingdom

Development of a rapid, sensitive and specific diagnostic assay for fish Aquareovirus based on RT-PCR

Characteristics of a new reovirus isolated from epizootic ulcerative syndrome infected snakehead fish

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