Aqueous biphasic system based on low-molecular-weight polyethylene glycol for one-step separation of crude polysaccharides from Pericarpium granati using high-speed countercurrent chromatography

Zhou, Xinyu; Zhang, Jing; Xu, Ruiping; Ma, Xue; Zhang, Zhiqi

doi:10.1016/j.chroma.2014.08.034

Cited by 26 publications

(8 citation statements)

References 31 publications

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“…However, it was not applied to a real polysaccharide sample . So far, there are only five publications concerned the separation of polysaccharide using HSCCC , and all of them were time consuming (e.g., approximately 4–20 h) when using a conventional HSCCC multilayer coil column . In this method, typical low elution rate such as 0.8 mL/min was used in a tube diameter 3.0 mm for the retain enough of the stationary phase [9].…”

Section: Resultsmentioning

confidence: 99%

“…However, spiral disk column require complex spiral grooves made in an inert plastic plate, which make commercial models more costly. Although previous studies have demonstrated that conventional multilayer coil columns can successfully separate polysaccharides, the separation process is long, for example, ≥10 h . Thus, polysaccharide separations based on an ATPS remain a challenge in HSCCC development, which highlights the need for further investigation.…”

Section: Introductionmentioning

confidence: 99%

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Rapid separation of polysaccharides using a novel spiral coil column by high-speed countercurrent chromatography

2016

J. Sep. Science

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The separation of polysaccharides is time consuming. We developed and optimized a type-J counter-current chromatography system with a novel tri-rotor spiral coil column for the rapid separation of polysaccharides. The optimal composition of an aqueous PEG1000/K2 HPO4 /KH2 PO4 system was found to be 14:16:14 w/w/w where the lower phase was the mobile phase. Optimal performance was achieved at a column rotational speed, temperature, and flow rate of 1200 rpm, 45°C, and 3.0 mL/min, respectively. The mobile phase was pumped from the inner terminal in a ''head-to-tail'' elution mode. Polysaccharide LCP-1 (10.7 mg) was successfully obtained in high purity in one step from 50.0 mg of a crude polysaccharide extracted from the lychee fruit (Litchi chinensis) within 100 min. LCP-1 possess a number-average molecular weight and weight-average molecular weight of 1.05 × 10(5) and 1.59 × 10(5) kDa, respectively. The monosaccharide composition consists of the molar ratio of glucose, galactose, and arabinose of 1.3:3.5:1.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid separation of polysaccharides using a novel spiral coil column by high-speed countercurrent chromatography

2016

J. Sep. Science

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“…Searching a suitable two‐phase solvent system for separating and purifying AC‐ARPS is the most important step in HSCCC, and the ten golden rules by Ito should be followed . The solvent system for HSCCC separation was selected by determining the layering time, pH, volume ratio of the upper and lower phases, and partition coefficient K of a series of solvent systems composed of different proportions of PEG‐phosphate system . The two‐phase system consisted of an aqueous polymer, which is composed of 12.5% w/w PEG 1000, 10% w/w potassium dihydrogen phosphate, and 10% w/w dipotassium hydrogen phosphate buffer at pH 6.8.…”

Section: Methodsmentioning

confidence: 99%

Purification of polysaccharide from artificially cultivated Anoectochilus roxburghii (wall.) Lindl . by high-speed counter current chromatography and its antitumor activity

Lin

Jinque

et al. 2017

J. Sep. Sci.

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To establish a systematic method for the extraction, purification, characterization and antitumor activity study of polysaccharide from artificially cultivated Anoectochilus roxburghii (wall.) Lindl. (AC-ARPS). High-speed counter current chromatography with two-phase aqueous systems was successfully applied to purify AC-ARPS after one-step separation. The purity of the AC-ARPS obtained by phenol/sulfuric acid method was 95.01%. The chemical structures of AC-ARPS were identified by a series of analytical methods including high-performance liquid chromatography and liquid chromatography with mass spectrometry. High-performance liquid chromatography and liquid chromatography with mass spectrometry indicated that AC-ARPS was mainly composed of mannose, ribose, glucose, galactose and arabinose with a molar ratio of 1.00:8.47:47.30:1.17:1.19. AC-ARPS is a homogeneous polysaccharide with a molecular weight of 25 681 Da. The antitumor effect of AC-ARPS was evaluated on lung cancer A549, osteosarcoma 143B, rat adrenal pheochromocytoma PC 12, breast cancer MCF-7, acute leukemia HL 60, chronic leukemia K562, colon cancer SW620, esophageal cancer OE 19, liver cancer HepG2, and neuroglioma U251 cells in vitro. AC-ARPS showed the best inhibitory effect on OE 19 cells, and the IC value was 5.67 ± 0.831 μmol/L. Fluorescence analysis and flow cytometry results showed that AC-ARPS induced apoptosis and G2/M phase arrest in OE 19 cells.

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“…Such mixtures are known as aqueous biphasic systems, as both layers are aqueous solutions of different compositions: the upper layer contains most of the PEG, whereas the lower layer contains most of the inorganic salt. Aqueous biphasic systems have proven to be useful in CCC for the purification of biological materials [ 121 , 135 – 137 ].…”

Section: Ionic Liquids In Chromatographymentioning

confidence: 99%

Ionic Liquid–Liquid Chromatography: A New General Purpose Separation Methodology

Brown¹,

Earle

Gîlea

et al. 2017

Top Curr Chem (Z)

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Ionic liquids can form biphasic solvent systems with many organic solvents and water, and these solvent systems can be used in liquid–liquid separations and countercurrent chromatography. The wide range of ionic liquids that can by synthesised, with specifically tailored properties, represents a new philosophy for the separation of organic, inorganic and bio-based materials. A customised countercurrent chromatograph has been designed and constructed specifically to allow the more viscous character of ionic liquid-based solvent systems to be used in a wide variety of separations (including transition metal salts, arenes, alkenes, alkanes, bio-oils and sugars).

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Aqueous biphasic system based on low-molecular-weight polyethylene glycol for one-step separation of crude polysaccharides from Pericarpium granati using high-speed countercurrent chromatography

Cited by 26 publications

References 31 publications

Rapid separation of polysaccharides using a novel spiral coil column by high-speed countercurrent chromatography

Rapid separation of polysaccharides using a novel spiral coil column by high-speed countercurrent chromatography

Purification of polysaccharide from artificially cultivated Anoectochilus roxburghii (wall.) Lindl . by high-speed counter current chromatography and its antitumor activity

Ionic Liquid–Liquid Chromatography: A New General Purpose Separation Methodology

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