Arabidopsis thaliana β-Glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides

Escamilla‐Treviño, Luis L.; Chen, Wei; Card, Marcella L.; Shih, Ming-Che; Cheng, Chi‐Lien; Poulton, Jonathan E.

doi:10.1016/j.phytochem.2006.05.022

Cited by 112 publications

(88 citation statements)

References 69 publications

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“…By contrast, BGLU46 has p-coumaryl alcohol glucoside as the preferred substrate, but nevertheless it displays a broad specificity toward various substrates such as coniferin, syringin, salicilin, arbutin, and phenyl b-D-glucosides (Xu et al, 2004;Escamilla-Treviño et al, 2006). The in vitro substrate specificity of BGLU45 and BGLU46 and the expression of their genes in stems (Escamilla-Treviño et al, 2006) support the hypothesis that these enzymes are involved in the lignification of Arabidopsis. To (1) verify whether coniferin is a true substrate for b-glucosidase in vivo and Figure 1.…”

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confidence: 77%

Impact of the Absence of Stem-Specific β-Glucosidases on Lignin and Monolignols

et al. 2012

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Monolignol glucosides are thought to be implicated in the lignin biosynthesis pathway as storage and/or transportation forms of cinnamyl alcohols between the cytosol and the lignifying cell walls. The hydrolysis of these monolignol glucosides would involve b-glucosidase activities. In Arabidopsis (Arabidopsis thaliana), in vitro studies have shown the affinity of b-GLUCOSIDASE45 (BGLU45) and BGLU46 for monolignol glucosides. BGLU45 and BGLU46 genes are expressed in stems. Immunolocalization experiments showed that BGLU45 and BGLU46 proteins are mainly located in the interfascicular fibers and in the protoxylem, respectively. Knockout mutants for BGLU45 or BGLU46 do not have a lignin-deficient phenotype. Coniferin and syringin could be detected by ultra-performance liquid chromatography-mass spectrometry in Arabidopsis stems. Stems from BGLU45 and BGLU46 mutant lines displayed a significant increase in coniferin content without any change in coniferyl alcohol, whereas no change in syringin content was observed. Other glucosylated compounds of the phenylpropanoid pathway were also deregulated in these mutants, but to a lower extent. By contrast, BGLU47, which is closely related to BGLU45 and BGLU46, is not implicated in either the general phenylpropanoid pathway or in the lignification of stems and roots. These results confirm that the major in vivo substrate of BGLU45 and BGLU46 is coniferin and suggest that monolignol glucosides are the storage form of monolignols in Arabidopsis, but not the direct precursors of lignin.

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confidence: 77%

Impact of the Absence of Stem-Specific β-Glucosidases on Lignin and Monolignols

et al. 2012

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“…The biological role of 4-O-glucosylated coniferyl and sinapyl alcohol (coniferin and syringin, respectively) is not yet understood (Escamilla-Treviño et al, 2006;Lanot et al, 2006;Vanholme et al, 2010b). According to current models, they might function either as storage or as transport forms (or both) of their corresponding aglycones, although no experimental proof is available (Samuels et al, 2002;Kaneda et al, 2008;Liu et al, 2011).…”

Section: Coniferin and Syringin As Storage Metabolitesmentioning

confidence: 99%

A Systems Biology View of Responses to Lignin Biosynthesis Perturbations inArabidopsis

et al. 2012

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Lignin engineering is an attractive strategy to improve lignocellulosic biomass quality for processing to biofuels and other bio-based products. However, lignin engineering also results in profound metabolic consequences in the plant. We used a systems biology approach to study the plant's response to lignin perturbations. To this end, inflorescence stems of 20 Arabidopsis thaliana mutants, each mutated in a single gene of the lignin biosynthetic pathway (phenylalanine ammonia-lyase1, and cinnamyl alcohol dehydrogenase6 [CAD6], two mutant alleles each), were analyzed by transcriptomics and metabolomics. A total of 566 compounds were detected, of which 187 could be tentatively identified based on mass spectrometry fragmentation and many were new for Arabidopsis. Up to 675 genes were differentially expressed in mutants that did not have any obvious visible phenotypes. Comparing the responses of all mutants indicated that c4h, 4cl1, ccoaomt1, and ccr1, mutants that produced less lignin, upregulated the shikimate, methyl-donor, and phenylpropanoid pathways (i.e., the pathways supplying the monolignols). By contrast, f5h1 and comt, mutants that provoked lignin compositional shifts, downregulated the very same pathways. Reductions in the flux to lignin were associated with the accumulation of various classes of 4-O-and 9-O-hexosylated phenylpropanoids. By combining metabolomic and transcriptomic data in a correlation network, system-wide consequences of the perturbations were revealed and genes with a putative role in phenolic metabolism were identified. Together, our data provide insight into lignin biosynthesis and the metabolic network it is embedded in and provide a systems view of the plant's response to pathway perturbations.

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“…β-Glycosidases activate β-glycosides, secondary metabolites that perform a wide array of cellular functions including the induction of symbiosis, breakdown of endosperm cell wall during germination, phytohormone activation, lignification, the production of aromatic compounds and defense (Nisius 1988;Poulton 1990;Dharmawardhana et al 1995;Leah et al 1995;Hungria & Stacey 1997;Kristoffersen et al 2000;Mizutani et al 2002;Morant et al 2003;Fia et al 2005;Maicas & Mateo 2005;Escamilla-Trevino et al 2006;Halkier & Gershenzon 2006;Lee et al 2006;Suzuki et al 2006;Naoumkina et al 2007;Stauber et al 2012). Some of the defense β-glycosides include α-hydroxynitrile glucosides, benzoxazinoid glycosides, avenacosides, isoflavonoid glycosides and glucosinolates (Niemeyer 1988;Nisius 1988;Poulton 1990;Cairns et al 2000;Halkier & Gershenzon, 2006).…”

Section: Introductionmentioning

confidence: 99%

The heterologous expression of aGlycine maxhomolog of NONEXPRESSOR OF PR1 (NPR1) and α-hydroxynitrile glucosidase suppresses parasitism by the root pathogenMeloidogyne incognitainGossypium hirsutum

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McNeece

Sharma

et al. 2016

Journal of Plant Interactions

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Experiments in Glycine max (soybean) identified the expression of the salicylic acid signaling and defense gene NONEXPRESSOR OF PR1 (NPR1) in root cells (i.e., syncytium) parasitized by the plant parasitic nematode Heterodera glycines undergoing the process of resistance. Gm-NPR1-2 overexpression in G. max effectively suppresses parasitism by H. glycines. The heterologous expression of Gm-NPR1-2 in Gossypium hirsutum impairs the ability of the parasitic nematode Meloidogyne incognita to form root galls, egg sacs, eggs and second-stage juvenile (J2) nematodes.In related experiments, a G. max β-glycosidase (Gm-βg-4) related to Lotus japonicus secreted defense gene α-hydroxynitrile glucosidase LjBGD7 suppresses M. incognita parasitism. The results identify a cumulative negative effect that the transgenes have on M. incognita parasitism and demonstrate that the G. max-H. glycines pathosystem is a useful tool to identify defense genes that function in other agriculturally relevant plant species to plant parasitic nematodes with different strategies of parasitism.ARTICLE HISTORY

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Arabidopsis thaliana β-Glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides

Cited by 112 publications

References 69 publications

Impact of the Absence of Stem-Specific β-Glucosidases on Lignin and Monolignols

Impact of the Absence of Stem-Specific β-Glucosidases on Lignin and Monolignols

A Systems Biology View of Responses to Lignin Biosynthesis Perturbations inArabidopsis

The heterologous expression of aGlycine maxhomolog of NONEXPRESSOR OF PR1 (NPR1) and α-hydroxynitrile glucosidase suppresses parasitism by the root pathogenMeloidogyne incognitainGossypium hirsutum

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