Arabinogalactan in banana: Chemical characterization and pharmaceutical effects

Guo, Wei; Rao, Guohua; Wen, Xu

doi:10.1016/j.ijbiomac.2020.11.060

Cited by 18 publications

(6 citation statements)

References 26 publications

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“…This result showed that the MDAE method did not change the primary structure of polysaccharides extracted from sweet tea [ 20 ]. More specifically, the signals at 5.26 ppm and 109.09 ppm suggested the presence of H-1 and C-1 of T-α-L-Ara f [ 39 , 40 ], respectively. The signals at 5.09 ppm and 107.28 ppm indicated the presence of H-1 and C-1 of 1,5-α-L-Ara f [ 34 , 39 ], respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Notably, the relative intensity of the signal at 3.80 ppm was higher in P-W compared to P-DM, which indicated that the esterification degree of P-W was higher than that of P-DM. Additionally, the signals at 4.53 ppm and 104.27 ppm indicated the presence of H-1 and C-1 of 1,3,6-β-D-Gal p [ 34 , 40 ], the signals at 4.46 ppm and 103.10 ppm suggested the presence of H-1 and C-1 of 1,6-β-D-Gal p , and the signals at 4.46 ppm and 104.27 ppm suggested the presence of H-1 and C-1 of 1,3-β-D-Gal p [ 34 , 43 ]. The signals at 2.15 ppm and 1.24 ppm suggested the presence of O-acetyl groups and H-6 of 1,2-α-L-Rha p , respectively [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

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Microwave-Assisted Deep Eutectic Solvent Extraction, Structural Characteristics, and Biological Functions of Polysaccharides from Sweet Tea (Lithocarpus litseifolius) Leaves

Guo

et al. 2022

Antioxidants

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The leaf of sweet tea (Lithocarpus litseifolius) is widely used as an edible and medicinal plant in China, which is rich in bioactive polysaccharides. In order to explore and promote the application of sweet tea polysaccharides in the functional food industry, the microwave-assisted deep eutectic solvent extraction (MDAE) of polysaccharides from sweet tea leaves was optimized, and the structural properties and biological functions of sweet tea polysaccharides prepared by MDAE (P-DM) were investigated and compared with that of hot water extraction (P-W). The maximum yield (4.16% ± 0.09%, w/w) of P-DM was obtained under the optimal extraction conditions (extraction time of 11.0 min, extraction power of 576.0 W, water content in deep eutectic solvent of 21.0%, and liquid–solid ratio of 29.0 mL/g). Additionally, P-DM and P-W possessed similar constituent monosaccharides and glycosidic bonds, and the homogalacturonan (HG) and arabinogalactan (AG) might exist in both P-DM and P-W. Notably, the lower molecular weight, higher content of total uronic acids, and higher content of conjugated polyphenols were observed in P-DW compared to P-W, which might contribute to its much stronger in vitro antioxidant, anti-diabetic, antiglycation, and prebiotic effects. Besides, both P-DW and P-W exhibited remarkable in vitro immunostimulatory effects. The findings from the present study indicate that the MDAE has good potential to be used for efficient extraction of bioactive polysaccharides from sweet tea leaves and P-DM can be developed as functional food ingredients in the food industry.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Microwave-Assisted Deep Eutectic Solvent Extraction, Structural Characteristics, and Biological Functions of Polysaccharides from Sweet Tea (Lithocarpus litseifolius) Leaves

Guo

et al. 2022

Antioxidants

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“…Combined with literature data [ 40 ] and methylation analysis, the signals derived from residue A were successfully assigned to →4)-α- d -Glcp-(1→. Likewise, the assignment of chemical shifts of residues B to F abided by the same routine [ 41 , 42 , 43 , 44 ], as shown in Table 2 . The downfield shifting of carbon signal of C-4 suggested a substitution on free hydroxyl group of C-4 of residues A , B and D .…”

Section: Resultsmentioning

confidence: 99%

“…These twelve correlation peaks in the anomeric region of AVPG-2, including δ 4.62/95.84 ppm, δ 5.40/99.70 ppm, δ 5.39/101.63 ppm, δ 5.22/108.27 ppm, δ 4.41/103.09 ppm, δ 5.23/109.18 ppm, δ 5.20/92.01 ppm, δ 5.07/98.92 ppm, δ 4.49/102.72 ppm, δ 4.71/100.70 ppm, δ 5.26/97.61 ppm and δ 5.06/107.43 ppm (denoted as A , B , C , D , E , F , G , H , I , J , K and L , respectively), revealed that there were twelve sugar residues in AVPG-2. The assignment of chemical shifts of these residues were determined according to 1 H, 13 C, HSQC, 1 H- 1 H COSY and HMBC spectra, as well as data reported in the literature [ 40 , 43 , 44 , 46 , 47 , 48 , 49 ] and the results of methylation analysis, as shown in Table 2 . The linkage sites and sequence among the sugar residues of AVPG-2 were also analyzed according to the HMBC spectra ( Figure 6 E).…”

Section: Resultsmentioning

confidence: 99%

Purification, Structural Characterization and Immunomodulatory Effects of Polysaccharides from Amomumvillosum Lour. on RAW 264.7 Macrophages

et al. 2021

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Amomum Villosum Lour. (A. villosum) is a folk medicine that has been used for more than 1300 years. However, study of the polysaccharides of A. villosum is seriously neglected. The objectives of this study are to explore the structural characteristics of polysaccharides from A. villosum (AVPs) and their effects on immune cells. In this study, the acidic polysaccharides (AVPG-1 and AVPG-2) were isolated from AVPs and purified via anion exchange and gel filtration chromatography. The structural characteristics of the polysaccharides were characterized by methylation, HPSEC-MALLS-RID, HPLC, FT-IR, SEM, GC-MS and NMR techniques. AVPG-1 with a molecular weight of 514 kDa had the backbone of → 4)-α-d-Glcp-(1 → 3,4)-β-d-Glcp-(1 → 4)-α-d-Glcp-(1 →. AVPG-2 with a higher molecular weight (14800 kDa) comprised a backbone of → 4)-α-d-Glcp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-d-Glcp-(1 →. RAW 264.7 cells were used to investigate the potential effect of AVPG-1 and AVPG-2 on macrophages, and lipopolysaccharide (LPS) was used as a positive control. The results from bioassays showed that AVPG-2 exhibited stronger immunomodulatory activity than AVPG-1. AVPG-2 significantly induced nitric oxide (NO) production as well as the release of interleukin (IL)-6 and tumor necrosis factor alpha (TNF-α), and upregulated phagocytic capacities of RAW 264.7 cells. Real-time PCR analysis revealed that AVPG-2 was able to turn the polarization of macrophages to the M1 direction. These results suggested that AVPs could be explored as potential immunomodulatory agents of the functional foods or complementary medicine.

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“…After methylation experiment and 2D NMR spectra elucidation, PSP was found to have the main chain composed of → 4)-Gal p -(1 → (59.2%) and some possible side chain units including Gal p -(1 → (7.8%), → 4,6)-Gal p -(1 → (6.2%), → 3,6)-Gal p -(1 → (2.7%), Ara f -(1 → (6.6%), → 5)-Ara f -(1 → (7.4%), and → 3,5)-Ara f -(1 → (5.0%). This arabinogalactan-type polysaccharide with a 1,4-galactan backbone was different with arabinogalactan from Cynanchum atratum ( 55 ) and Carthamus tinctorius L. (with 1,3-galactan backbone) ( 56 ), and banana ( 57 ).…”

Section: Physicochemical and Structural Featuresmentioning

confidence: 90%

Advances in Polygonatum sibiricum polysaccharides: Extraction, purification, structure, biosynthesis, and bioactivity

et al. 2022

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Polygonatum sibiricum has been used as food and medicine for thousands of years, and P. sibiricum polysaccharides (PSPs) have become the hot research spot due to their various health-promoting functions. Numerous studies have shown that PSPs possess huge potential in the application of functional food and medicine fields. However, the research status and features of the preparation process, molecular structure, and bioactivities of PSPs are unclear. Therefore, this review makes a comprehensive summary and proposes new insights and guidelines for the extraction, purification, structural features, biosynthesis, and multiple bioactivities of PSPs. Notably, it is concluded that PSPs mainly contain several types of polysaccharides, including fructan, pectin, galactomannan, glucomannans, arabinogalactan, and galactan, and multiple bioactivates, including osteogenic activity, anti-obesity, anti-diabetes, anti-depression, antioxidant, antiglycation, and protective effect against neurotoxicity and gut microbiota regulating activity. This review contributes to the structure–function study and resource utilization of P. sibiricum and its polysaccharides in food fields.

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Arabinogalactan in banana: Chemical characterization and pharmaceutical effects

Cited by 18 publications

References 26 publications

Microwave-Assisted Deep Eutectic Solvent Extraction, Structural Characteristics, and Biological Functions of Polysaccharides from Sweet Tea (Lithocarpus litseifolius) Leaves

Microwave-Assisted Deep Eutectic Solvent Extraction, Structural Characteristics, and Biological Functions of Polysaccharides from Sweet Tea (Lithocarpus litseifolius) Leaves

Purification, Structural Characterization and Immunomodulatory Effects of Polysaccharides from Amomumvillosum Lour. on RAW 264.7 Macrophages

Advances in Polygonatum sibiricum polysaccharides: Extraction, purification, structure, biosynthesis, and bioactivity

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