Arbovirus Surveillance near the Mexico–U.S. Border: Isolation and Sequence Analysis of Chikungunya Virus from Patients with Dengue-like Symptoms in Reynosa, Tamaulipas

Laredo-Tiscareño, S. Viridiana; Machaín-Williams, Carlos; Rodríguez‐Pérez, Mario A.; Garza-Hernández, Javier A.; Doria-Cobos, Gloria L.; Cetina-Trejo, Rosa C.; Bacab-Cab, Lucio A.; Tangudu, Chandra S.; Charles, Jermilia; Luna-Santillana, Erick de Jesús de; Garcia-Rejon, Julián E.; Blitvich, Bradley J.

doi:10.4269/ajtmh.18-0117

Cited by 13 publications

(12 citation statements)

References 12 publications

(16 reference statements)

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“…Diversion of vector bites to non-human competent hosts reduces viral amplification and reduces potentially infectious bites to humans by dengue vectors, thus could be used as a control tool to limit arbovirus transmission. Although an earlier study in a similar Reynosa neighborhood isolated CHIKV from patients with dengue-like symptoms [56], and co-circulation of DENV1-3 and West Nile virus [57], all pools of host-seeking Ae. aegypti were negative for the presence of arbovirus RNA (DENV 1-4, ZIKV, and CHIKV), and all pools of Cx.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 87%

Vertebrate-Aedes aegypti and Culex quinquefasciatus (Diptera)-arbovirus transmission networks: Non-human feeding revealed by meta-barcoding and next-generation sequencing

et al. 2020

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Background Aedes aegypti mosquito-borne viruses including Zika (ZIKV), dengue (DENV), yellow fever (YFV), and chikungunya (CHIKV) have emerged and re-emerged globally, resulting in an elevated burden of human disease. Aedes aegypti is found worldwide in tropical, sub-tropical, and temperate areas. The characterization of mosquito blood meals is essential to understand the transmission dynamics of mosquito-vectored pathogens. Methodology/principal findings Here, we report Ae. aegypti and Culex quinquefasciatus host feeding patterns and arbovirus transmission in Northern Mexico using a metabarcoding-like approach with next-generation deep sequencing technology. A total of 145 Ae. aegypti yielded a blood meal analysis result with 107 (73.8%) for a single vertebrate species and 38 (26.2%) for two or more. Among the single host blood meals for Ae. aegypti, 28.0% were from humans, 54.2% from dogs, 16.8% from cats, and 1.0% from tortoises. Among those with more than one species present, 65.9% were from humans and dogs. For Cx. quinquefasciatus, 388 individuals yielded information with 326 (84%) being from a single host and 63 (16.2%) being from two or more hosts. Of the single species blood meals, 77.9% were from dogs, 6.1% from chickens, 3.1% from house sparrows, 2.4% from humans, while the remaining 10.5% derived from other 12 host species. Among those which had fed on more than one species, 11% were from dogs and humans, and 89% of other host species combinations. Forage ratio analysis revealed dog as the most over-utilized host by Ae. aegypti (= 4.3) and Cx. quinquefasciatus (= 5.6) and the human blood index at 39% and 4%, respectively. A total of 2,941 host-seeking female Ae. aegypti and 3,536 Cx. quinquefasciatus mosquitoes were collected in the surveyed area. Of these, 118 Ae. aegypti pools and 37 Cx. quinquefasciatus pools were screened for seven arboviruses (ZIKV, DENV 1–4, CHIKV, and West Nile virus (WNV)) using qRT-PCR and none were positive (point prevalence = 0%). The 95%-exact upper limit confidence interval was 0.07% and 0.17% for Ae. aegypti and Cx. quinquefasciatus, respectively Conclusions/significance The low human blood feeding rate in Ae. aegypti, high rate of feeding on mammals by Cx. quinquefasciatus, and the potential risk to transmission dynamics of arboviruses in highly urbanized areas of Northern Mexico is discussed.

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Section: Plos Neglected Tropical Diseasesmentioning

confidence: 87%

Vertebrate-Aedes aegypti and Culex quinquefasciatus (Diptera)-arbovirus transmission networks: Non-human feeding revealed by meta-barcoding and next-generation sequencing

et al. 2020

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“…We excluded vaccine strains, synthetic strains, and other non-wild type sequences (Table S2). We included sequences of the three previously described CHIKV genotypes (West Africa, ECSA, and Asian) and the 24 previously reported Mexican CHIKV sequences from Yucatán, northern and southern Chiapas, Jalisco, and Tamaulipas [13,15,29,30]. We combined the 22 newly obtained sequences with the retrieved CHIKV sequences to generate the Complete data set ( n = 900).…”

Section: Methodsmentioning

confidence: 99%

Genetic Variability of Chikungunya Virus in Southern Mexico

Galán-Huerta

Zomosa-Signoret

Vidaltamayo

et al. 2019

Viruses

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Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes Chikungunya fever. CHIKV entered Mexico through the state of Chiapas in October 2014. To fully understand the Chikungunya fever outbreak that occurred in southern Chiapas during 2015, we evaluated 22 PCR-confirmed CHIKV-positive patients, identified CHIKV genetic variability, reconstructed viral dispersal, and assessed possible viral mutations. Viruses were isolated and E2, 6K, and E1 genes were sequenced. We applied phylogenetic and phylogeographic approaches, modeled mutations, and estimated selective pressure. Different CHIKV strains circulated in Chiapas during summer 2015. Three isolates grouped themselves in a well-supported clade. Estimates show that the outbreak started in Ciudad Hidalgo and posteriorly dispersed towards Tapachula and neighboring municipalities. We found six non-synonymous mutations in our isolates. Two mutations occurred in one isolate and the remaining mutations occurred in single isolates. Mutations E2 T116I and E2 K221R changed the protein surface in contact with the host cell receptors. We could not find positive selected sites in our CHIKV sequences from southern Chiapas. This is the first viral phylogeographic reconstruction in Mexico characterizing the CHIKV outbreak in southern Chiapas.

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“…In addition, we recently detected several cases of non-travel-acquired chikungunya virus (CHIKV; genus Alphavirus, family Togaviridae) in Tamaulipas in 2015. 26 Taken together, we provide evidence for the co-circulation of five arboviruses of medical concern in a single season in Tamaulipas, northern Mexico.…”

Section: Discussionmentioning

confidence: 53%

“…In addition, we recently reported autochthonous transmission of CHIKV, an Alphavirus, in Tamaulipas. 26 All of the aforementioned viruses were associated with human disease in the study area, demonstrating the important need to continue performing vigilant arbovirus surveillance and diagnosis on the Mexico-U.S. border.…”

Section: Discussionmentioning

confidence: 93%

Surveillance for Flaviviruses Near the Mexico–U.S. Border: Co-circulation of Dengue Virus Serotypes 1, 2, and 3 and West Nile Virus in Tamaulipas, Northern Mexico, 2014–2016

Laredo-Tiscareño

Garza-Hernández

Salazar

et al. 2018

The American Journal of Tropical Medicine and Hygiene

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A clinical, serological, and molecular investigation was performed to determine the presence of dengue virus (DENV) and other flaviviruses among residents of the city of Reynosa, Tamaulipas, on the Mexico-U.S. border in 2014-2016. The sample population consisted of 2,355 patients with suspected dengue, in addition to 346 asymptomatic individuals recruited during a household-based epidemiological investigation designed to identify flavivirus seroconversions. Sera were collected from patients with suspected dengue in the acute phase of illness and from asymptomatic individuals at enrollment and every 5-7 months for 19 months. Sera from suspected dengue patients were tested for DENV antigen by enzyme-linked immunosorbent assay (ELISA), and select antigen-positive sera were further tested using a serotype-specific, quantitative reverse transcription-polymerase chain reaction. Sera from the household cohort were tested for flavivirus-reactive antibodies by immunoglobulin (Ig) M and IgG ELISAs using DENV antigen. A total of 418 (17.7%) patients with suspected dengue had laboratory-confirmed DENV infections, including 82 patients who were positive for DENV RNA. The most frequently detected serotype was DENV-1 (61 patients), followed by DENV-2 (16 patients) and DENV-3 (five patients). A total of 217 (62.7%) asymptomatic individuals had flavivirus-reactive antibodies at enrollment, and nine flavivirus-naïve individuals seroconverted. Sera from a subset of dengue patients and household participants, including all those who seroconverted, were further tested by plaque reduction neutralization test, resulting in the detection of antibodies to DENV-1, DENV-2, and West Nile virus. In summary, we provide evidence for the co-circulation of multiple flaviviruses in Reynosa, Tamaulipas, on the Mexico-U.S. border.

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Arbovirus Surveillance near the Mexico–U.S. Border: Isolation and Sequence Analysis of Chikungunya Virus from Patients with Dengue-like Symptoms in Reynosa, Tamaulipas

Cited by 13 publications

References 12 publications

Vertebrate-Aedes aegypti and Culex quinquefasciatus (Diptera)-arbovirus transmission networks: Non-human feeding revealed by meta-barcoding and next-generation sequencing

Vertebrate-Aedes aegypti and Culex quinquefasciatus (Diptera)-arbovirus transmission networks: Non-human feeding revealed by meta-barcoding and next-generation sequencing

Genetic Variability of Chikungunya Virus in Southern Mexico

Surveillance for Flaviviruses Near the Mexico–U.S. Border: Co-circulation of Dengue Virus Serotypes 1, 2, and 3 and West Nile Virus in Tamaulipas, Northern Mexico, 2014–2016

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