Arcanobacterium wilhelmae sp. nov., isolated from the genital tract of a rhinoceros (Rhinoceros unicornis)

Abstract

A taxonomic study using a polyphasic approach was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium isolated from the genital tract of a rhinoceros. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strains of Arcanobacterium canis (98.8 % 16S rRNA gene sequence similarity), Arcanobacterium phocisimile (97.8 %), Arcanobacterium phocae (97.7 %), Arcanobacterium haemolyticum (97.4 %), Arcanobacterium hippocoleae (96.6 %), Arcanobacterium pinnipediorum (96.4 %) and Arcarnobacterium pluranimalium (95.4 %). DNA-DNA hybridization values between strain 647T and Arcanobacterium canisDSM 25104T were very low, 13.4 % (reciprocal 15.9 %). The genomic DNA G+C content of strain 647T was 58.7 mol%. The presence of the major menaquinone MK-9(H4) supported the affiliation of this strain to the genus Arcanobacterium. The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine and an unidentified phosphoglycolipid. The results of physiological and biochemical testing clearly distinguished the unknown bacterium from other species of the genus Arcanobacterium. Based on these tests, it is proposed that the unknown bacterium should be classified as a representative of a novel species of the genus Arcanobacterium named Arcanobacterium wilhelmaesp. nov. The type strain is 647T (=DSM 102162T=LMG 29418T).

Arcanobacterium buesumense sp. nov., isolated from an anal swab of a male harbour seal (Phoca vitulina)

“…The major components were C 16 : 0 (30.94%), C 18 : 1 ω9c (30.44%) and C 18 : 0 (18.29%), which was congruent with the fatty acid profiles of other species of the genus Arcanobacterium [2,4,7]. Fatty acid profiles published by Hijazin et al [4] and Sammra et al [7][8][9] were analysed in the same laboratory as the data represented here by the use of biomass grown under the same conditions and harvested at the same growth stage.…”

“…These authors proposed that genus Arcanobacterium should be split into two genera, with Arcanobacterium haemolyticum , Arcanobacterium phocae , Arcanobacterium pluranimalium and Arcanobacterium hippocoleae belonging to the genus Arcanobacterium sensu stricto and Arcanobacterium abortisuis , Arcanobacterium bernardiae , Arcanobacterium bialowiezense , Arcanobacterium bonasi and Arcanobacterium pyogenes being transferred to he genus Trueperella . More recently, Arcanobacterium canis and Arcanobacterium phocisimile [3, 4], Arcanobacterium urinimassiliense [5], Arcanobacterium ihumii [6], Arcanobacterium pinnipediorum [7], Arcanobacterium wilhelmae and Arcanobacterium bovis [8, 9] were described.…”

Arcanobacterium bovis sp. nov., isolated from the milk of a cow with mastitis

“…Arcanobacterium abortisuis, Arcanobacterium bernardiae, Arcanobacterium bialowiezense, Arcanobacterium bonasi and Arcanobacterium pyogenes being transferred to a new genus, Trueperella and Arcanobacterium haemolyticum, Arcanobacterium phocae, Arcanobacterium pluranimalium and Arcanobacterium hippocoleae constituting he genus Arcanobacterium. More recently, the novel species Arcanobacterium canis, Arcanobacterium phocisimile, Arcanobacterium pinnipediorum, Arcanobacterium wilhelmae, Arcanobacterium urinimassiliense and Arcanobacterium ihumii were described [3][4][5][6][7][8].…”

“…Biomass subjected to extraction of quinones and polar lipids was grown in brain heart infusion broth with 5 % bovine serum at 37 °C with shaking and harvested at the same growth phase as reported for the other species of genus Arcanobacterium [3][4][5][6]. Isoprenoid quinones and polar lipids were extracted and analysed by applying an integrated method described by Tindall [28,29] and Altenburger et al [30].…”

“…info) and corresponded to the description of the genus Arcanobaterium as rectified by Yassin et al [1]. Physiological characterization was implemented as described previously [3][4][5][6]; results for strain C605018/01/1 T are given in the species description and correlate with the type strains of species of the genus Arcanobacterium in Table 1.…”