Arohynapenes A and B, new anticoccidial agents produced by Penicillium sp. Taxonomy, fermentation, and structure elucidation.

Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites

“…corrugata and identified by analysis of spectroscopic data along with comparison with literature data (Sundholm, 1978). The structure and absolute stereochemistry of (S)-8-methoxy-3,5-dimethylisochroman-6-ol (2), isolated from P. steckii obtained from an alga belonging to the genus Sargassum, could be established by analysis of spectroscopic data and also by comparison with literature data (Lai et al, 1990;Masuma et al, 1994;He et al, 2004;Kerti et al, 2007). A Penicillium sp.…”

“…The organic phase was evaporated to dryness, to give 57 mg of a crude extract which displayed antibiotic activity against Candida albicans ATCC36801 and Streptoccocus mutans UA159 and presented a 1 H NMR spectrum of a single aromatic compound contaminated with fatty acids. HPLC purification of this crude extract (column: C 18 Inertsil ODS-3, 4.6 x 250 mm, 5 μm; eluent: 75:25 MeOH/H 2 O; flow: 1 mL/min) led to the isolation of (S)-8-methoxy-3,5-dimethylisochroman-6-ol (2) (Lai et al, 1990;Masuma et al, 1994;He et al, 2004;Kerti et al, 2007 (Masuma et al, 1994;Cox et al, 1979) did not allow us to distinguish it from 6-methoxy-3,7-dimethylisochroman-8-ol (3) (Cox et al, 1979). Therefore, compound 2 was identified by analysis of COSY, HSQC and HMBC NMR spectra.…”

“…Therefore, compound 2 was identified by analysis of COSY, HSQC and HMBC NMR spectra. The absolute configuration of 2 was established by comparison of its [α]D 25 +142 (c 0.1, MeOH) with the literature values (Lai et al, 1990;Masuma et al, 1994;He et al, 2004;Kerti et al, 2007).…”

Antiparasitic agents produced by microorganisms

“…26) No active member from a new class of chemical substances had been developed for more than 10 years, so our group screened anticoccidial antibiotics using monensin-resistant Eimeria tenella in vitro. As a result, we isolated several new antibiotics, including xanthoquinodins, 27) diolmycins, 28) hynapenes, 29) arohynapenes, 30) cytosaminomycins, 31), 32) and fudecalone. 33) Xanthoquinodin A1 (11), produced by a fungus (Humicola sp.…”

Screening of Antianaerobic Antibiotics and Anticoccidial Antibiotics from Microorganisms.

“…We studied various assay systems by a plate method and devised a Reverse layer method as shown in Fig. 2, which , Herbimycins 8) , Karabemycin 9) (4) Antiviral antibiotics: Virantmycin 10) , Virustomycin 11) (5) Antimycoplasmal antibiotics: 2'-Amino-2'-deoxyadenosine 12) , Cervinomycins 13) , Deoxyfrenolicin 14) , Frenolicin B 14) , OM-173 15) (6) Antianaerobic antibiotics: Clostomicins 16) , Luminamicin 17) , Lustromycin 18) , Thiotetromycin 19) (7) Anticoccidial antibiotics: Cytosaminomycins 20) , Diolmycins 21) , Frenolicin B *22) , Arohynapenes 23) , Fudecalone 24) , Hynapenes 25) , Xanthoquinodins 26) *This antibiotic was isolated during the course of screening for (5) antimycoplasmal antibiotics.…”