a b s t r a c tMining activities including mineral excavation and ore transportation produce and release large amounts of pollutants to the surrounding environment, raising concerns regarding the effects of environmental exposure to pollutants on human health. The concentration of elements in hair can be used as a biomarker of exposure to chemical elements. In the present study, hair samples from 89 relatively healthy volunteers aged 11-77 years old (57 living near Bayan Obo giant REE-Nb-Fe ore deposit and 32 in non-mining areas) from Baotou (Inner Mongolia, China) were analyzed to determine the contents of 25 chemical elements (Ag, Al, As, Ba, Bi, Ca, Cd, Co, Cr, Cs, Cu, Fe, Ga, Li, Mg, Mn, Mo, Ni, Pb, Rb, Se, Tl, U, V and Zn) with the aim of gaining insights about the potential exposure to chemical elements of the population living within the Bayan Obo opencast mining area. The effects of gender and living area on element concentrations in hair were also investigated. The results showed that the mean concentrations of the target elements (Ag, Al, Ba, Cd, Co, Li, Mn, Ni, Pb, Tl, V, Cs, Ga, Rb, U, Bi, Mo, As, Ca, Fe, Mg and Zn) in human hair from the mining area (MA) were primarily higher than that in non-mining areas (NMA). The area of residence had an influence on the presence of select trace elements (As, Cd, Mg, Pb, Tl, U and Zn) in human hair. In addition, the hair samples from MA showed statistically significant differences between males and females for a larger number of elements than those in NMA in which only Ag, Bi and Se were significantly different. This result implies a potential influence of mining activities on residents living in the vicinity. These findings confirm the need for competent authorities to act as early as possible and to implement strategies aimed to protect exposed populations and the entire ecosystem.