Arsenic toxicity: cell signalling and the attenuating effect of nitric oxide in Eichhornia crassipes

Andrade, Heloísa Monteiro de; Oliveira, Juraci Alves de; Farnese, Fernanda dos Santos; Ribeiro, Cléberson; Silva, Antônio Alberto da; Neto, José Lino

doi:10.1007/s10535-015-0572-4

Cited by 27 publications

(9 citation statements)

References 40 publications

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“…Therefore, SOD activity results in increased H 2 O 2 generation and must be accompanied by an increase in the activity of enzymes responsible for scavenging H 2 O 2 , such as CAT and POX. The increase of SOD activity in response to As toxicity observed in this study has also been reported in other plants, such as Lactuca sativa (Gusman et al 2013) and Eichhornia crassipes (Andrade et al 2016). However, when As levels are very high, the extent of the damage caused by the pollutant decreases enzyme activity.…”

Section: Discussionsupporting

confidence: 85%

“…Accordingly, these enzymes were important in A. caroliniana for defence against ROS at low As concentrations, but in higher concentrations the toxic effects of the pollutant inactivated the enzymes. Similar results were observed in rice (Shri et al 2009) and aquatic plants (Farnese et al 2014;Andrade et al 2016) after exposure to high As concentrations. Given that plant growth was maintained even in the highest concentrations of the metalloid, it is likely that A. caroliniana uses other strategies for survival.…”

Section: Discussionsupporting

confidence: 80%

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Phytoremediation of arsenic-contaminated water: the role of antioxidant metabolism of Azolla caroliniana Willd. (Salviniales)

et al. 2017

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Phytoremediation has proven to be an effi cient technology for removing arsenic (As) from water, but the plants used in this process need to be tolerant to the damage caused by As. Th e toxic eff ect of As on growth and functioning of the antioxidant system was studied in individual plants of Azolla caroliniana exposed to fi ve concentrations of As (0.0, 0.25, 0.5, 1.0 and 1.5 mg L -1 ) for the course of fi ve days. Growth, As absorption, enzymatic activity, total and non-protein thiols and anthocyanin content were assessed. Azolla caroliniana was able to take up large amounts of the pollutant, reaching As concentrations of 386.1 µg g -1 dry weight without saturating the absorption mechanism. Th e tolerance index and the growth of A. caroliniana decreased with the increased As uptake. Superoxide dismutase, peroxidases, catalases and glutathione reductase activities increased at lower doses of As and subsequently declined with higher concentrations, whereas ascorbate peroxidase activity was reduced in all treatments. Unlike the enzymatic defence system, anthocyanin and thiol content increased consistently in all treatments and showed a positive correlation with As concentration. Th erefore, the increased synthesis of non-enzymatic antioxidants is most likely the main factor responsible for the high As tolerance of A. caroliniana.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 80%

Phytoremediation of arsenic-contaminated water: the role of antioxidant metabolism of Azolla caroliniana Willd. (Salviniales)

et al. 2017

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“…Nitric oxide is a cell signaling molecule particularly important in the plant’s tolerance to stress. In the last few years, several researches showed that NO is able to improve plant tolerance to As ( Shukla et al, 2015 ; Silveira et al, 2015 ; Andrade et al, 2016 ). Strong evidences indicate that NO acts as a second messenger, triggering different cellular responses, such as the increase in antioxidant defense systems ( Fan et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

The Involvement of Nitric Oxide in Integration of Plant Physiological and Ultrastructural Adjustments in Response to Arsenic

et al. 2017

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High arsenic (As) concentrations are toxic to all the living organisms and the cellular response to this metalloid requires the involvement of cell signaling agents, such as nitric oxide (NO). The As toxicity and NO signaling were analyzed in Pistia stratiotes leaves. Plants were exposed to four treatments, for 24 h: control; SNP [sodium nitroprusside (NO donor); 0.1 mg L-1]; As (1.5 mg L-1) and As + SNP (1.5 and 0.1 mg L-1, respectively). The absorption of As increased the concentration of reactive oxygen species and triggered changes in the primary metabolism of the plants. While photosynthesis and photorespiration showed sharp decrease, the respiration process increased, probably due to chemical similarity between arsenate and phosphate, which compromised the energy status of the cell. These harmful effects were reflected in the cellular structure of P. stratiotes, leading to the disruption of the cells and a possible programmed cell death. The damages were attenuated by NO, which was able to integrate central plant physiological processes, with increases in non-photochemical quenching and respiration rates, while the photorespiration level decreased. The increase in respiratory rates was essential to achieve cellular homeostasis by the generation of carbon skeletons and metabolic energy to support processes involved in responses to stress, as well to maintaining the structure of organelles and prevent cell death. Overall, our results provide an integrated view of plant metabolism in response to As, focusing on the central role of NO as a signaling agent able to change the whole plant physiology.

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“…At low concentrations, NO contributes to increased tolerance of the plants to metals in various ways, for example, by promoting metal binding to the cell wall, preventing their entry into the cell ( Singh et al, 2011 ) or promoting their compartmentalization in the vacuole, either by increasing phytochelatin synthesis ( De Michele et al, 2009 ) or by altering the activity of proton pumps in the vacuolar membrane to create an electrochemical gradient that favors the absorption of metals ( Cui et al, 2010 ). In addition to these effects, NO can also reprogram plant physiological processes and stimulate the synthesis and activity of antioxidant systems, which is essential to limit the oxidative stress induced by metals ( Cheng et al, 2015 ; Andrade et al, 2016 ). Finally, post-translational changes triggered by NO can decrease the activity of enzymes involved in ROS metabolism, such as glycolate oxidase and NADPH oxidase, allowing the cell to re-establish redox homeostasis ( Yun et al, 2011 ; Quiang et al, 2012 ).…”

Section: Crosstalk Between Ros and No In The Response To Abiotic Strementioning

confidence: 99%

When Bad Guys Become Good Ones: The Key Role of Reactive Oxygen Species and Nitric Oxide in the Plant Responses to Abiotic Stress

Farnese

Menezes‐Silva

Gusman³

et al. 2016

Front. Plant Sci.

263

134

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The natural environment of plants is composed of a complex set of abiotic stresses and their ability to respond to these stresses is highly flexible and finely balanced through the interaction between signaling molecules. In this review, we highlight the integrated action between reactive oxygen species (ROS) and reactive nitrogen species (RNS), particularly nitric oxide (NO), involved in the acclimation to different abiotic stresses. Under stressful conditions, the biosynthesis transport and the metabolism of ROS and NO influence plant response mechanisms. The enzymes involved in ROS and NO synthesis and scavenging can be found in different cells compartments and their temporal and spatial locations are determinant for signaling mechanisms. Both ROS and NO are involved in long distances signaling (ROS wave and GSNO transport), promoting an acquired systemic acclimation to abiotic stresses. The mechanisms of abiotic stresses response triggered by ROS and NO involve some general steps, as the enhancement of antioxidant systems, but also stress-specific mechanisms, according to the stress type (drought, hypoxia, heavy metals, etc.), and demand the interaction with other signaling molecules, such as MAPK, plant hormones, and calcium. The transduction of ROS and NO bioactivity involves post-translational modifications of proteins, particularly S-glutathionylation for ROS, and S-nitrosylation for NO. These changes may alter the activity, stability, and interaction with other molecules or subcellular location of proteins, changing the entire cell dynamics and contributing to the maintenance of homeostasis. However, despite the recent advances about the roles of ROS and NO in signaling cascades, many challenges remain, and future studies focusing on the signaling of these molecules in planta are still necessary.

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Arsenic toxicity: cell signalling and the attenuating effect of nitric oxide in Eichhornia crassipes

Cited by 27 publications

References 40 publications

Phytoremediation of arsenic-contaminated water: the role of antioxidant metabolism of Azolla caroliniana Willd. (Salviniales)

Phytoremediation of arsenic-contaminated water: the role of antioxidant metabolism of Azolla caroliniana Willd. (Salviniales)

The Involvement of Nitric Oxide in Integration of Plant Physiological and Ultrastructural Adjustments in Response to Arsenic

When Bad Guys Become Good Ones: The Key Role of Reactive Oxygen Species and Nitric Oxide in the Plant Responses to Abiotic Stress

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