Artemisia capillaris inhibits atopic dermatitis-like skin lesions in Dermatophagoides farinae-sensitized Nc/Nga mice

Abstract

BackgroundArtemisia capillaries Thunb. (AC) has been used to treat inflammatory and hepatic disorders such as hepatic injury, hepatic fibrosis and hepatitis. However, the efficacy of AC against atopic dermatitis (AD), an inflammatory disease, has not been examined. In the present study, AC was evaluated for anti-inflammatory and anti-AD effects using both in vitro and in vivo systems.MethodsThe contents of six compounds (chlorogenic acid, caffeic acid, isochlorogenic acid A, hyperoside, isoquercitrin and scoparone) in AC were simultaneously assayed using HPLC system. To evaluate the anti-inflammatory effect of AC, NO production was measured in RAW264.7 cell stimulated with 1 μg/mL LPS. Histamine levels were assayed in MC/9 cells stimulated with 50 nM PMA and 1 μM A23187. To examine the role of AC in vivo, AC (10 mg/mouse/day) was topically applied for four weeks the back and ears of Dermatophagoides farinae-sensitized Nc/Nga mice. Protopic ointment (0.1% tacrolimus) was used as a positive control.ResultsThe contents of the six components in AC range from 0.44 to 43.14 mg/g. Chlorogenic acid (21.06 ± 0.08 mg/g) and isochlorogenic acid A (43.14 ± 0.12 mg/g) were major components in AC. AC inhibited NO and histamine production in cells respectively. In D. farinae-sensitized Nc/Nga mice, the topical application of AC reduced dermatitis scores, hemorrhage, hypertrophy and hyperkeratosis of the epidermis in the dorsal skin and ear. The treatment of AC also reduced the plasma levels of histamine (1.5 fold) and IgE (1.4 fold).ConclusionsOur results suggest that AC should be explored as a potential therapeutic agent to treat atopic dermatitis and analysis by HPLC will help to improve the quality of AC.

Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process

“…YC mainly contains phenolic acids, flavonoids, and coumarins [4][5][6][7]. And phenolic acids are proved in previous studies to have various biological functions, including antioxidant [8,9], anti-inflammatory [10], antibacterial [11], cytoprotective [9], hepatoprotective [12,13], and anti-osteoporosis [14,15] effects.…”

Antiproliferation of keratinocytes and alleviation of psoriasis by the ethanol extract of Artemisia capillaris

“…Artemisia capillaris (AC) Thunberg has been used as food additives and traditional phytomedicines. The major components of AC are chlorogenic acids, coumarins, and flavonoids (Ha, Lee, Seo, et al, ; Okuno, Uchida, Nakamura, & Sakurawi, ). AC exhibited therapeutic potentials for the treatment of cancer, hepatitis, inflammation, malaria, obesity, and microbial infections (Aniya, Shimabukuro, Shimoji, et al, ; Cha, Moon, Kim, Cha, & Lee, ; Hong, Hwang, Kim, Jeong, & Lee, ).…”

“…It was reported that the application of the extract of AC (ACE) can reduce interleukin‐1beta (IL‐1β) and interferon‐gamma‐induced nitric oxide (NO) production, which may be correlated well with the reduced expression levels of the inducible nitric oxide synthase (iNOS) mRNA and protein (Kim, Kwon, Han, et al, ). Related to the treatment of skin disorders, AC inhibited atopic dermatitis‐like skin lesions in Dermatophagoides farinae ‐sensitized Nc/Nga mice (Ha et al, ). The pharmacological active components were extracted with ethanol from the whole part of AC in this study and the antipsoriatic potentials of ACE were first, to the best of our knowledge, demonstrated in imiquimod (IMQ)‐induced psoriasis‐like mouse models.…”

A Survey of Therapeutic Effects ofArtemisia capillarisin Liver Diseases

“…Currently, a number of constituents including p -hydroxyacetophenone, β -sitosterol, scoparone, cirsimaritin, quercetin, arcapillin, capillin, 6,7-dimethylesculetin, 6,7-dimethoxycoumarin, capillone, capillarin, 4′-methyl capillarisin, cirsilineol, cirsimaritin, and capillarisin from A. capillaris have been shown to have antihepatofibrotic, anti-inflammatory, choleretic, and hepatoprotective activities [ 6 – 10 ]. Furthermore, more diverse pharmacological properties of A. capillaris have been studied in areas such as lipoapoptosis, obesity, skin cancer, antibacterial activities, and atopic dermatitis, as well as liver protection [ 11 – 15 ].…”