Artemisinin: present status and perspectives

Dhingra, Vikas; Rao, K. Vishweshwar; Narasu, M. Lakshmi

doi:10.1016/s0307-4412(98)00282-9

Cited by 20 publications

(14 citation statements)

References 13 publications

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“…Artemisinin, a sesquiterpene lactone endoperoxide, is a promising antimalarial obtained from the leaves of a Chinese herb, Artemisia annua L. This drug is shown to possess considerable activity against a wide range of organisms including Plasmodium falciparum (1)(2)(3). It is very effective in the treatment of cerebral malaria.…”

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confidence: 99%

Purification and Characterization of an Enzyme Involved in Biochemical Transformation of Arteannuin B to Artemisinin from Artemisia annua

Dhingra

Narasu

2001

Biochemical and Biophysical Research Communications

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confidence: 99%

Purification and Characterization of an Enzyme Involved in Biochemical Transformation of Arteannuin B to Artemisinin from Artemisia annua

Dhingra

Narasu

2001

Biochemical and Biophysical Research Communications

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“…In the artemisinin biosynt h e s i s p a t h w a y, o n l y a m o r p h a -4 , 11 -d i e n e (Bouwmeester et al 1999), artemisinic acid (Sangwan et al 1993), dihydroartemisinic acid (Wallaart et al 1999a), dihydroartemisinic acid hydroperoxide (Wallaart et al 1999b), and arteannuin B (Nair and Basile 1993;Dhingra et al 2000;Dhingra and Narasu 2001) are known to serve as intermediates downstream to the common precursor FPP. However, the biosynthetic pathways suggested by various groups are contradictory (Dhingra et al 2000) and other intermediates are not yet clear. In the past few years, amorpha-4,11-diene synthase, which catalyses the cyclization of FPP to amorpha-4,11-diene, was cloned and functionally expressed in E. coli and tobacco (Mercke et al 2000;Wallaart et al 2001).…”

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confidence: 99%

Advances in the Plant Isoprenoid Biosynthesis Pathway and Its Metabolic Engineering

Liu

Wang

et al. 2005

J Integrative Plant Biology

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Although the cytosolic isoprenoid biosynthetic pathway, mavolonate pathway, in plants has been known for many years, a new plastidial 1-deoxyxylulose-5-phosphate (DXP) pathway was identified in the past few years and its related intermediates, enzymes, and genes have been characterized quite recently. With a deep insight into the biosynthetic pathway of isoprenoids, investigations into the metabolic engineering of isoprenoid biosynthesis have started to prosper. In the present article, recent advances in the discoveries and regulatory roles of new genes and enzymes in the plastidial isoprenoid biosynthesis pathway are reviewed and examples of the metabolic engineering of cytosolic and plastidial isoprenoids biosynthesis are discussed.

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“…The mechanism of artemisinin action is based on digestion of hemoglobin leading to the release of iron containing heme. [2] The heme group will then which induce oxidative stress and, therefore, selective alkylation of parasite proteins causing the death of malaria parasite. [2] Although artemisinin absorbs light in the UV region between 190 and 210 nm, its extinction coefficient is poor.…”

Section: Introductionmentioning

confidence: 99%

ANALYSIS OF ARTEMISININ ISOLATED FROM Artemisia Annua L. BY TLC AND HPLC

Ghafoori

Sariri

Naghavi

et al. 2013

Journal of Liquid Chromatography & Related Technologies

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& Artemisia annua L. (Quinghao) is a promising and potent source of antimalarial herbal drug. Its activity has been ascribed to the content of artemisinin; it has been analyzed by different chromatographic techniques. In this research, we have developed a TLC=UV, a simply available, rapid, and cost-efficient method, for the simultaneous determination of artemisinin. The crude extracts from plant samples were used together with a standard in our HPLC=UV and TLC= UV techniques. Artemisinin (standard and in the extract) was converted into a UV-absorbing compound, Q258 by being treated with 0.25% (by weight) NaOH solution and then 0.2 M acetic acid. The resulting TLC method utilizes separation on silica gel RP-18 with methanol, acetonitrile, ethyl acetate, acetic acid (30:20:2:1) as mobile phase, highly specific densitometric evaluation at 254 nm. This precise and accurate assay could be performed in the linear working range of 10-100 lg=mL and 1-40 lg=mL for TLC and HPLC methods, respectively. The new technique has enabled us to screen high artemisinin producing plants from a large number of samples.

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Artemisinin: present status and perspectives

Cited by 20 publications

References 13 publications

Purification and Characterization of an Enzyme Involved in Biochemical Transformation of Arteannuin B to Artemisinin from Artemisia annua

Purification and Characterization of an Enzyme Involved in Biochemical Transformation of Arteannuin B to Artemisinin from Artemisia annua

Advances in the Plant Isoprenoid Biosynthesis Pathway and Its Metabolic Engineering

ANALYSIS OF ARTEMISININ ISOLATED FROM Artemisia Annua L. BY TLC AND HPLC

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