Arthrobacter nitrophenolicus sp. nov. a new 2-chloro-4-nitrophenol degrading bacterium isolated from contaminated soil

Arora, Pankaj; Jain, Rakesh

doi:10.1007/s13205-012-0066-4

Cited by 21 publications

(15 citation statements)

References 13 publications

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“…Analysis. All 4C2NP-decolorizing bacteria were identified on the basis of 16S rRNA gene sequencing using the universal primers 27f and 1492r as described previously [13,14]. The 16S rRNA gene sequences of related strains were retrieved from the NCBI and aligned using the ClusterW program.…”

Section: Identification Of 4-chloro-2-nitrophenol-decolorizing Bactermentioning

confidence: 99%

Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample

Arora¹,

Srivastava²,

Singh³

2016

Journal of Chemistry

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Eighteen bacterial strains, isolated from a waste water sample collected from a chemically contaminated site, Patancheru (17°32′N 78°16′E/17.53°N 78.27°E), India, were able to decolorize 4-chloro-2-nitrophenol (4C2NP) in the presence of an additional carbon source. These eighteen 4C2NP-decolorizing strains have been identified as members of four different genera, includingBacillus,Paenibacillus,Pseudomonas, andLeuconostocbased on the 16S rRNA gene sequencing and phylogenetic analysis. Most of the bacteria (10) belonged to the genusBacillusand contributed 56% of the total 4C2NP-degrading bacteria, whereas the members of generaPaenibacillusandPseudomonasrepresented 22% and 17%, respectively, of total 4C2NP-degrading isolates. There was only one species ofLeuconostoccapable of degrading 4C2NP. This is the first report of the diversity of 4C2NP-decolorizing bacteria in a waste water sample. Furthermore, one bacterium,Bacillus aryabhattaistrain PC-7, was able to decolorize 4C2NP up to a concentration of 2.0 mM. Gas chromatography-mass spectrometry analysis identified 5-chloro-2-methylbenzoxazole as the final product of 4C2NP decolorization in strain PC-7.

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Section: Identification Of 4-chloro-2-nitrophenol-decolorizing Bactermentioning

confidence: 99%

Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample

Arora¹,

Srivastava²,

Singh³

2016

Journal of Chemistry

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“…The bacterial strain PA-2 used in this study was isolated from soil collected from Yeungnam University. This strain was identified based on the 16S rRNA gene sequence using universal primers as previously described [13,14].…”

Section: Bacterial Strainmentioning

confidence: 99%

Chemotaxis Away from 4-Chloro-2-nitrophenol, 4-Nitrophenol, and 2,6-Dichloro-4-nitrophenol byBacillus subtilisPA-2

Arora

Jeong

Bae

2015

Journal of Chemistry

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Bacterial strain PA-2 exhibits chemotaxis away from 4-chloro-2-nitrophenol, 4-nitrophenol, and 2,6-dichloro-4-nitrophenol. This strain was identified asBacillus subtilison the basis of the 16S rRNA gene sequencing. The drop plate assay and the chemical-in-plug method were used to demonstrate negative chemotactic behavior of strain PA-2. The growth studies showed that strain PA-2 did not utilize 4-chloro-2-nitrophenol, 4-nitrophenol, and 2,6-dichloro-4-nitrophenol as its sole sources of carbon and energy. This is the first report of negative chemotaxis of 4-chloro-2-nitrophenol, 4-nitrophenol, and 2,6-dichloro-4-nitrophenol by any bacterium.

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“…The difference is in the change of the positions of the chloro and nitro groups at the benzene ring. There are several bacteria that utilize 2C4NP as their sole carbon and energy sources, including Arthrobacter nitrophenolicus SJCon [6,12], Burkholderia sp. SJ98 [9], Burkholderia sp.…”

Section: Introductionmentioning

confidence: 99%

Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, Exiguobacterium sp. PMA

et al. 2012

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BackgroundChloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however.ResultsA 4C2NP-degrading bacterium, Exiguobacterium sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. Exiguobacterium sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. Exiguobacterium sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of Exiguobacterium sp. PMA. The bioremediation of 4C2NP by Exiguobacterium sp. PMA was faster in non-sterilized soil than sterilized soil.ConclusionsOur studies indicate that Exiguobacterium sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.

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Arthrobacter nitrophenolicus sp. nov. a new 2-chloro-4-nitrophenol degrading bacterium isolated from contaminated soil

Cited by 21 publications

References 13 publications

Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample

Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample

Chemotaxis Away from 4-Chloro-2-nitrophenol, 4-Nitrophenol, and 2,6-Dichloro-4-nitrophenol byBacillus subtilisPA-2

Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, Exiguobacterium sp. PMA

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