Articular Cartilage Repair Using Tissue Engineering Technique—Novel Approach with Minimally Invasive Procedure

Ochi, Mitsuo; Adachi, Nobuo; Nobuto, Hiroo; Yanada, Shinobu; Itô, Yôhei; Agung, Muhammad

doi:10.1111/j.1525-1594.2004.07317.x

Cited by 131 publications

(91 citation statements)

References 17 publications

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“…Unfortunately, the long-term therapeutic efficacy of these methods is controversial (8). Regenerative medicine using autologous chondrocyte implantation (ACI) is currently of great interest for the treatment of cartilage damage because it is less invasive and is biocompatible in vivo for longer periods of time compared with the therapies that are currently available (9). In ACI, a biopsy of the cartilage is taken from the patient's knee by an arthroscopic surgery.…”

Section: Introductionmentioning

confidence: 99%

Effects of insulin-like growth factor 1 and basic fibroblast growth factor on the morphology and proliferation of chondrocytes embedded in Matrigel in a microfluidic platform

Fan

Jiang

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. An integrated microfluidic device was utilized in the present study to investigate the morphology and proliferation of rabbit articular chondrocytes embedded in Matrigel in the presence of insulin-like growth factor 1 (IGF-1) and/or basic fibroblast growth factor (bFGF). The microfluidic device was composed of two parallel channels and a central perfusion-based three-dimensional cell culture module. The rabbit chondrocytes were cultured for 2 weeks at series of concentration gradients of IGF-1 and/or bFGF, which were generated through a diffusion process. At the end of the experiment, the morphology and quantity of cells were measured. Since high expression of collagen II is essential to the function of hyaline cartilage, immunofluorescent images of collagen II expression prior to and after the experiments were gathered for each group. The mean fluorescence intensity ratio (MIR) of collagen II in each group was calculated. The MIRs of collagen II in chondrocytes treated with IGF-1 ranged from 0.6-0.81, those in the cells treated with bFGF ranged from 0.47-0.52, and those in cells treated with a combination of IGF-1 and bFGF ranged from 0.63-0.83. Chondrocyte aggregations were observed in the group treated with 75-100 ng/ml IGF-1 (3.46-fold proliferation ratio). Similarly, a 3.83-fold proliferation ratio was identified in chondrocytes treated with 2.5-5.0 ng/ml bFGF. The group treated with 50-75 ng/ml IGF-1 and 2.5-5.0 ng/ml bFGF exhibited the optimum increase in proliferation (4.83-fold proliferation ratio). The microfluidic device used in the present study can be easily adapted to investigate other growth factors at any concentration gradient. In addition, parallel experiments can be performed simultaneously with a small quantity of cells, making it an attractive platform for the high-throughput screening of cell culture parameters. This platform will aid in the optimization of culture conditions for the in vitro expansion of chondrocytes while maintaining their in vivo morphology, which will improve autologous chondrocyte implantation capabilities for the treatment of cartilage injury.

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Section: Introductionmentioning

confidence: 99%

Effects of insulin-like growth factor 1 and basic fibroblast growth factor on the morphology and proliferation of chondrocytes embedded in Matrigel in a microfluidic platform

Fan

Jiang

et al. 2017

Experimental and Therapeutic Medicine

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“…Based on these qualities of MSCs, the regeneration of bone and cartilage has been studied (Ochi et al 2004;Petite et al 2000). Fibroblast growth factor-2 (FGF-2) is involved in angiogenesis and tissue repair.…”

Section: Introductionmentioning

confidence: 99%

FGF-2 increases osteogenic and chondrogenic differentiation potentials of human mesenchymal stem cells by inactivation of TGF-β signaling

et al. 2007

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Human mesenchymal stem cells (hMSCs) are able to self-replicate and differentiate into a variety of cell types including osteoblasts, chondrocytes, adipocytes, endothelial cells, and muscle cells. It was reported that fibroblast growth factor-2 (FGF-2) increased the growth rate and multidifferentiation potentials of hMSCs. In this study, we investigated the genes involved in the promotion of osteogenic and chondrogenic differentiation potentials of hMSCs in the presence of FGF-2. hMSCs were maintained in the medium with FGF-2. hMSCs were harvested for the study of osteogenic or chondrogenic differentiation potential after 15 days' culture. To investigate osteogenic differentiation, the protein levels of alkaline phosphatase (ALP) and the mRNA expression levels of osteocalcin were measured after the induction of osteogenic differentiation. Moreover, the investigation for chondrogenic differentiation was performed by measuring the mRNA expression levels of type II and type X collagens after the induction of chondrogenic differentiation. The expression levels of ALP, type II collagen, and type X collagen of hMSCs cultured with FGF-2 were significantly higher than control. These results suggested that FGF-2 increased osteogenic and chondrogenic differentiation potentials of hMSCs. Furthermore, microarray analysis was performed after 15 days' culture in the medium with FGF-2. We found that the overall insulin-like growth factor-I (IGF-I) and transforming growth factor-b (TGF-b) signaling pathways were inactivated by FGF-2. These results suggested that the inactivation of IGF-I and TGF-b signaling promotes osteogenic and chondrogenic differentiation potential of hMSCs in the presence of FGF-2.

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“…The AC presents a notable matrix structure organization [6], a limited number of chondrocytes [7], and a rich water content [8]. The most important biomechanical functions of the AC include weight bearing and a smooth distribution of forces to the adjacent subchondral bone, providing nonfrictional motion of joints [4,8].…”

Section: Osteoarticular Disorders and Regeneration Physiologymentioning

confidence: 99%

“…The unique matrix structure, rich in collagen fibers, proteoglycans, and interstitial fiber, provides a viscoelastic environment that allows the AC to support its biomechanical functions [8]. It has been well established that the AC has limited self-healing capacities [3][4][5][7][8][9][10][11][12][13][14][15] due to its intrinsic characteristics, namely its avascular nature, limited number of resident stem cells, and unique matrix organization [4]. Partial defects on mature cartilage do not heal spontaneously.…”

Section: Osteoarticular Disorders and Regeneration Physiologymentioning

confidence: 99%

Synovia-Derived Mesenchymal Stem Cell Application in Musculoskeletal Injuries: A Review

Branquinho¹,

Caseiro²,

SantosPedrosa³

et al. 2018

Tissue Regeneration

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Musculoskeletal injuries impact millions of people globally and affect their health and well-being as well as of their companion and athletic animals. Soft-tissue injuries represent almost half of these and are associated with unorganized scar tissue formation and long time-depending healing processes. Cell-based therapeutic strategies have been developed in the past decades aiming at the treatment and reversion of such disorders. Stem cells are fairly appealing in the field, being a responsive undifferentiated population, with ability to self-renew and differentiate into different lineages. Mesenchymal stem cells (MSCs) can be obtained from several adult tissues, including the synovial membrane. Synovia-derived MSCs can be found in individuals of any age and are associated to intrinsic regenerative processes, through both paracrine and cell-to-cell interactions, thus, contributing to hosts' healing capacity. Studies have demonstrated the potential benefit of synovia-derived MSCs in these regenerative processes in both human and veterinary medicine. The purpose of this chapter is to review the literature regarding SM-MSC therapies applied to musculoskeletal disorders, in both human and veterinary medicine.

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Articular Cartilage Repair Using Tissue Engineering Technique—Novel Approach with Minimally Invasive Procedure

Cited by 131 publications

References 17 publications

Effects of insulin-like growth factor 1 and basic fibroblast growth factor on the morphology and proliferation of chondrocytes embedded in Matrigel in a microfluidic platform

Effects of insulin-like growth factor 1 and basic fibroblast growth factor on the morphology and proliferation of chondrocytes embedded in Matrigel in a microfluidic platform

FGF-2 increases osteogenic and chondrogenic differentiation potentials of human mesenchymal stem cells by inactivation of TGF-β signaling

Synovia-Derived Mesenchymal Stem Cell Application in Musculoskeletal Injuries: A Review

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