Ascorbic acid drives the differentiation of mesoderm‐derived embryonic stem cells. Involvement of p38 MAPK/CREB and SVCT2 transporter

Rahman, Fryad; Bordignon, Benoît; Culerrier, Raphaël; Peiretti, Franck; Spicuglia, Salvatore; Djabali, Malek; Landrier, Jean‐François; Fontés, Michel

doi:10.1002/mnfr.201600506

Cited by 18 publications

(12 citation statements)

References 35 publications

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“…is concentration may induce a switch in hGF activity from cell viability and migration to ECM production. Our findings revealed that the effects of vitamin C were dose-dependent corresponding with the findings of Rahman et al [24]. e higher concentrations of vitamin C, resulting in reduced cell viability, are unresolved.…”

Section: Discussionsupporting

confidence: 81%

Rinsing with L-Ascorbic Acid Exhibits Concentration-Dependent Effects on Human Gingival Fibroblast In Vitro Wound Healing Behavior

Chaitrakoonthong

Ampornaramveth

Kamolratanakul

2020

International Journal of Dentistry

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Vitamin C or L-ascorbic acid has diverse functions in the body, especially healing promotion in tissue injury via participating in the hydroxylation reactions required for collagen formation. Systemic administration of vitamin C plays an important role on gingival fibroblast proliferation and functions. Whether local or rinsing administration of vitamin C alters gingival fibroblast wound healing behavior remains unclear. The aim of this study was to investigate the rinsing effect of vitamin C on gingival fibroblast behavior utilizing an in vitro wound healing model. Primary human gingival fibroblasts isolated from gingival tissue were rinsed with medium containing various concentrations of vitamin C. The rinsing effect of vitamin C on in vitro wound healing was assessed using a scratch test assay. Cell migration, cell viability, and extracellular matrix gene expression were analyzed by transwell migration assay, MTT assay, and real-time RT-PCR, respectively. We found that rinsing with 10 or 20 µg/ml vitamin C significantly increased fibroblast migration (p≤0.05). However, no significant effect was found in the cell viability or in vitro wound healing assays. In contrast, rinsing with 50 µg/ml vitamin C significantly delayed wound closure (p≤0.05). Real-time PCR demonstrated that 50 µg/ml vitamin C significantly increased fibroblast expression of COL1, FN, IL-6, and bFGF. The data demonstrate that rinsing with vitamin C (10/20 µg/ml) accelerates fibroblast migration. However, 50 µg/ml of vitamin C increases the expression of COL1, FN, IL-6, and bFGF, which are related to fibroblast wound healing activity. Prescribing vitamin C with the appropriate duration and drug administration method should be determined to maximize its benefit.

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Section: Discussionsupporting

confidence: 81%

Rinsing with L-Ascorbic Acid Exhibits Concentration-Dependent Effects on Human Gingival Fibroblast In Vitro Wound Healing Behavior

Chaitrakoonthong

Ampornaramveth

Kamolratanakul

2020

International Journal of Dentistry

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“…Differentiation and regenerative capabilities of BMSCs can be enhanced by manipulating the cellular micro-environment. One goal of regenerative medicine is to optimize the regenerative potential of BMSCs through nutritional supplementation [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. Vitamin C is one of the most important players in cell proliferation, differentiation, extracellular matrix (ECM) synthesis and cytoskeletal development [ 21 , 22 , 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Micronutrients, including vitamin C, are important factors in musculoskeletal development and BMSC biology [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. For example, vitamin C is a key player in collagen synthesis, cell proliferation, and BMSC differentiation [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Modulation of miRNAs by Vitamin C in Human Bone Marrow Stromal Cells

et al. 2018

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MicroRNAs (miRNAs) are small (18–25 nucleotides), noncoding RNAs that have been identified as potential regulators of bone marrow stromal cell (BMSC) proliferation, differentiation, and musculoskeletal development. Vitamin C is known to play a vital role in such types of biological processes through various different mechanisms by altering mRNA expression. We hypothesized that vitamin C mediates these biological processes partially through miRNA regulation. We performed global miRNA expression analysis on human BMSCs following vitamin C treatment using microarrays containing human precursor and mature miRNA probes. Bioinformatics analyses were performed on differentially expressed miRNAs to identify novel target genes and signaling pathways. Our bioinformatics analysis suggested that the miRNAs may regulate multiple stem cell-specific signaling pathways such as cell adhesion molecules (CAMs), fatty acid biosynthesis and hormone signaling pathways. Furthermore, our analysis predicted novel stem cell proliferation and differentiation gene targets. The findings of the present study demonstrate that vitamin C can have positive effects on BMSCs in part by regulating miRNA expression.

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“…The effect of AA in the context of body weight could be at least partially related to stem cell differentiation towards myogeneis and osteogenesis, inhibiting adipogenesis. Since aging is associated with sarcopenia and defects in body weight, the current observations suggest that AA-mediated stem cell effects could play a role in the aging process [ 36 , 37 ].…”

Section: Ascorbic Acid and The Aging Process: In Vitro Modelsmentioning

confidence: 99%

Vitamin C, Aging and Alzheimer’s Disease

et al. 2017

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Accumulating evidence in mice models of accelerated senescence indicates a rescuing role of ascorbic acid in premature aging. Supplementation of ascorbic acid appeared to halt cell growth, oxidative stress, telomere attrition, disorganization of chromatin, and excessive secretion of inflammatory factors, and extend lifespan. Interestingly, ascorbic acid (AA) was also found to positively modulate inflamm-aging and immunosenescence, two hallmarks of biological aging. Moreover, ascorbic acid has been shown to epigenetically regulate genome integrity and stability, indicating a key role of targeted nutrition in healthy aging. Growing in vivo evidence supports the role of ascorbic acid in ameliorating factors linked to Alzheimer’s disease (AD) pathogenesis, although evidence in humans yielded equivocal results. The neuroprotective role of ascorbic acid not only relies on the general free radical trapping, but also on the suppression of pro-inflammatory genes, mitigating neuroinflammation, on the chelation of iron, copper, and zinc, and on the suppression of amyloid-beta peptide (Aβ) fibrillogenesis. Epidemiological evidence linking diet, one of the most important modifiable lifestyle factors, and risk of Alzheimer's disease is rapidly increasing. Thus, dietary interventions, as a way to epigenetically modulate the human genome, may play a role in the prevention of AD. The present review is aimed at providing an up to date overview of the main biological mechanisms that are associated with ascorbic acid supplementation/bioavailability in the process of aging and Alzheimer’s disease. In addition, we will address new fields of research and future directions.

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Ascorbic acid drives the differentiation of mesoderm‐derived embryonic stem cells. Involvement of p38 MAPK/CREB and SVCT2 transporter

Cited by 18 publications

References 35 publications

Rinsing with L-Ascorbic Acid Exhibits Concentration-Dependent Effects on Human Gingival Fibroblast In Vitro Wound Healing Behavior

Rinsing with L-Ascorbic Acid Exhibits Concentration-Dependent Effects on Human Gingival Fibroblast In Vitro Wound Healing Behavior

Modulation of miRNAs by Vitamin C in Human Bone Marrow Stromal Cells

Vitamin C, Aging and Alzheimer’s Disease

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