Ashwagandha-polyphenols-functionalized gold nanoparticles facilitate apoptosis by perturbing microtubule assembly dynamics in breast cancer cells

Antiproliferative efficacy and mechanism of action of garlic phytochemicals-functionalized gold nanoparticles in triple-negative breast cancer cells

“…For example, as we have reported earlier, tryptone-stabilized gold nanoparticles eliminate triple-negative breast cancer (MDA-MB-231) by inducing unipolar clustering of supernumerary centrosomes [10]. Surface-functionalization of gold nanoparticles with the active components of the plants augmented their anti-proliferative, and anti-migratory effects, as we demonstrated subsequently with Triphala-and ashwagandha-coated gold nanoparticles [11,12]. Phytochemicals assist in the reduction and stabilization of gold [13].…”

“…Phytochemicals assist in the reduction and stabilization of gold [13]. Moreover, the phytochemicals from the medicinal plant extract act in combination with the gold nanoparticles to inhibit cancer cell proliferation [11,12].…”

Induction of autophagy-dependent and caspase- and microtubule-acetylation-independent cell death by phytochemical-stabilized gold nanopolygons in colorectal adenocarcinoma cells

“…17,18 Therefore, Brahmi was selected as the source of the phytochemicals for reduction and functionalization. We had previously shown the collective functionalization of the polyphenols of Triphala 21 and Ashwagandha phytochemicals 22 on gold nanoparticles, their anticancer effect, and their ability to kill triple-negative breast cancer cells, MDA-MB-231. Here we report the strong antiproliferative efficacy and a previously unknown but therapeutically relevant mechanism of action of these particles in HT29 cancer cells.…”

“…40 Thus, in vitro, tubulin-Bm-GNP binding analyses suggest that the nanoparticles could bind to tubulin and slightly but consistently disrupt the secondary structure of the protein without altering the tertiary structure. Phytochemicals 40,41 in general, and several phytochemicalbased inorganic nanoformulations in particular, 21,22 have been shown not to be grossly damaging to tubulin but perturbed them sufficiently enough to induce cell cycle arrest. In order to verify the role of tubulin disruption in the inhibition of cell viability, we further investigated the intracellular manifestations of the binding interactions of Bm-GNPs on cellular microtubules at their IC 50 for cell proliferation.…”

An overview on the exploring the interaction of inorganic nanoparticles with microtubules for the advancement of cancer therapeutics

“…Also, it has been shown that Withania somnifera (WS)-synthesized AuNPs (25 nm) could show anticancer effects through MT disassembly dynamicsmediated apoptosis (Fig. 4b) [95].…”