Assessing Insecticide Resistance in Adult Mosquitoes: Perspectives on Current Methods

Richards, Stephanie L.; Byrd, Brian D; Reiskind, Michael H.; White, Avian V

doi:10.1177/1178630220952790

Cited by 31 publications

(27 citation statements)

References 17 publications

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“…Nevertheless, results obtained from the laboratory assessment must be interpreted with caution as the tests were conducted in a well-controlled environment when making operational field decisions. A variety of biological parameters including type of strain and preference of egg-laying oviposition, as well as environmental parameters such as temperature, humidity, rainfall, and sunlight conditions, can all affect the efficacy of the product [ 63 – 65 ]. Despite laboratory tests revealing the minor repellent impact of MHAQ against mosquitoes, the number of eggs obtained in small-scale field trials was higher than the gold standard (ovitrap).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a mosquito home system for controlling Aedes aegypti

et al. 2021

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Background Dengue is a significant public health issue that is caused by Aedes spp. mosquitoes. The current vector control methods are unable to effectively reduce Aedes populations and thus fail to decrease dengue transmission. Hence, there is an urgent need for new tools and strategies to reduce dengue transmission in a wide range of settings. In this study, the Mosquito Home System (MHS) and Mosquito Home Aqua (MHAQ) formulations were assessed as commercial autodissemination traps in laboratory and small-scale field trials. Method Multiple series of laboratory and small-scale field trials were performed to assess the efficacy of MHS and MHAQ exposed to Ae. aegypti. In the laboratory trials, various parameters such as fecundity, fertility, wing size, oviposition preferences, residual effects, and MHAQ transference to other containers through controlled experiments were tested. For small-scale field trials, the efficacy of the MHS and MHAQ approaches was determined to ascertain whether wild mosquitoes could transfer the MHAQ formulation from MHS stations to ovitraps. Results The data revealed that Ae. aegypti was highly susceptible to low concentrations of MHAQ formulations and had a residual effect of up to 3 months, with MHAQ exposure affecting fecundity, fertility, and mosquito wing size. In the oviposition studies, gravid females strongly preferred the hay infusion compared to tap water and MHAQ during egg-laying in the laboratory. Nevertheless, the use of commercial MHAQ by MHS was highly attractive in field settings compared to conventional ovitraps among local Aedes spp. mosquitoes. In addition, MHAQ horizontal transfer activities in the laboratory and small-scale field trials were demonstrated through larval bioassays. These findings demonstrated the potential of MHAQ to be transferred to new containers in each study site. Conclusion This study provided proof of principle for the autodissemination of MHAQ. Through further refinement, this technique and device could become an effective oviposition trap and offer an alternative preventive tool for vector control management. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Evaluation of a mosquito home system for controlling Aedes aegypti

et al. 2021

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“…Surveillance of resistance development to many different insecticides are determined by dose-mortality bioassays, the World Health Organization tube testing, and Centers for Disease Control and Prevention (CDC) bottle bioassay for mosquitoes [11,44,194,195]. In the dose-mortality assay, the resistance ratio (RR) is determined in a susceptible population to monitor changes in resistance over time.…”

Section: Effective Use Of Plant-based Bioinsecticides In Resistant Mo...mentioning

confidence: 99%

“…Thus, insecticide resistance is now considered a serious threat to control mosquito invasion and disease transmission. It is essential that the methods for insecticide monitoring in mosquito populations and interpretation of results are performed adequately, effectively and in a timely manner for improving mosquito control [10,11].…”

Section: Introductionmentioning

confidence: 99%

Plant-Based Bioinsecticides for Mosquito Control: Impact on Insecticide Resistance and Disease Transmission

Demirak

Canpolat

2022

Insects

103

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The use of synthetic insecticides has been a solution to reduce mosquito-borne disease transmission for decades. Currently, no single intervention is sufficient to reduce the global disease burden caused by mosquitoes. Problems associated with extensive usage of synthetic compounds have increased substantially which makes mosquito-borne disease elimination and prevention more difficult over the years. Thus, it is crucial that much safer and effective mosquito control strategies are developed. Natural compounds from plants have been efficiently used to fight insect pests for a long time. Plant-based bioinsecticides are now considered a much safer and less toxic alternative to synthetic compounds. Here, we discuss candidate plant-based compounds that show larvicidal, adulticidal, and repellent properties. Our discussion also includes their mode of action and potential impact in mosquito disease transmission and circumvention of resistance. This review improves our knowledge on plant-based bioinsecticides and the potential for the development of state-of-the-art mosquito control strategies.

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“…Five-day-old adult female mosquitoes of the parental and isofemale colonies were used for insecticide-susceptibility assays as per WHO guidelines. Insecticide-impregnated papers of discriminating doses and insecticide resistance monitoring test kit ordered from Vector Control Research, Universiti Sains Malaysia (a WHO referral Centre for these kits and impregnated papers) [33]. Each replicate consisted of 25 females and four such replicates were used for the experiment.…”

Section: Insecticide-susceptibility Assaysmentioning

confidence: 99%

Enrichment of phenotype among biological forms ofAnopheles stephensiListon through establishment of isofemale lines

Ghosh

Kumar

Kushwah

et al. 2022

Preprint

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The success of vector management programs relies on knowledge of the biology and genetic make-up of mosquitoes so that they can be interlaced with modern tools for developing suitable intervention strategies. There are many reports available for rearing varied species of mosquito vectors. However, there are limited studies addressing the development of isofemale lines among mosquitoes to homogenize the population to obtain both high-quality genome assemblies and enrichment of phenotype. Anopheles stephensi, an urban malaria vector, is one of the major invasive vectors of malaria distributed throughout the Indian subcontinent, Middle East, and has recently been expanding its range in Africa. With the existence of three biological forms, distinctly identifiable based on the number of ridges on eggs with varying vectorial competence, An. stephensi is a perfect species for developing a method for the successful establishment of isofemale lines, which can be tested for retention of the expected vectorial competence for the various forms. We describe the key steps in the establishment and validation of isofemale lines, which include monitoring the transgenerational fitness traits, morphometrics of eggs, and adult wing size during every generation. After the initial inbreeding depression, as proof of the tedious selection process, no significant morphometric differences were observed in the wings and egg size between the parental and their respective isofemale lines. We observed a significant change in the vectorial competence between the respective isofemale and parental lines enriching expected differential susceptibility towards malaria parasites by the type and intermediate forms. Interestingly, IndCh and IndInt strains showed variations in resistance to different insecticides belonging to all the four major classes. These variant lines have been characterized for their levels of homozygosity both at the phenotype and genotype levels and can be used as a standard reference or as a biological resource for other studies related to urban malaria research.

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Assessing Insecticide Resistance in Adult Mosquitoes: Perspectives on Current Methods

Cited by 31 publications

References 17 publications

Evaluation of a mosquito home system for controlling Aedes aegypti

Evaluation of a mosquito home system for controlling Aedes aegypti

Plant-Based Bioinsecticides for Mosquito Control: Impact on Insecticide Resistance and Disease Transmission

Enrichment of phenotype among biological forms ofAnopheles stephensiListon through establishment of isofemale lines

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