Assessing wheat (Triticum aestivum) genotypes for “Yr” resistance genes using conserved regions and simple-sequence motifs

Abstract

ABSTRACT. Analysis of DNA sequence variation among genotypes is useful for differentiation of wheat accessions, selection strategies and genetic development of crop plants. We screened molecular markers for yellow rust resistance genes (Yr7, Yr9, Yr15, Yr18, Yr26, and YrH52), which are in the gene-rich regions of wheat chromosomes 1B, 2B, and 7D, to investigate DNA sequence differences and repeat motifs and numbers between wheat cultivars resistant (Izgi2001, Sonmez2001, PI178383) and susceptible (Aytin98, ES14, Harmankaya99) to yellow rust. The F 2 individuals derived from the crosses were evaluated for yellow rust resistance at both the seedling and adult stages to identify DNA markers genetically linked to yellow rust resistance. The most repeated motif was found to be GA and the least repeated motif TAGA among the cultivars. When we examined DNA sequence differences (insertion, deletion and single nucleotide changes), the molecular markers Xgwm526 (Yr7) and Xgwm273 (YrH52) were found to have the most conserved regions and Yr15 (Xgwm413) the least conserved regions among the cultivars. This DNA sequence information can be used for selection of suitable parents, creating mapping populations and developing molecular markers associated with yellow rust resistance in plant breeding programs.

The Efficacity Investigation for Some Markers Detecting Yellow Rust Resistance Genes in Bread Wheat Varieties

“…The product size for this marker is consistent with the results obtained by Çabuk et al (2011). The same result was obtained for the PI178383 variety used in their studies [19]. The results of both studies confirm each other.…”

Haplotype analysis of Ethiopian bread wheat (Triticum aestivum) cultivars and elite lines for yellow rust resistance genes using linked molecular markers

“…It is common in CIMMYT-originated bread wheat cultivars. SSR marker Xgwm582 and a resistance gene analogue (RGA) clone marker (iag95) were linked to the Yr9 gene (Cabuk et al, 2011;Mago et al, 2002). Marker Xgwm582 amplified three bands (142, 149 and 152 bp) on the reference line.…”

“…Additionally, their co-segregation with other traits such as leaf tip necrosis (Ltn1), powdery mildew resistance gene (Pm38), and tolerance to barley yellow dwarf virus (Bdv1) has been reported (Liang et al, 2006;Singh, 1992a;Spielmeyer et al, 2005). Wms298 (Cabuk et al, 2011) and a gene specific marker L34DINT9F, L34PLUSR (Krattinger et al, 2009) were used to haplotype this gene. Of the 74 entries, 33 (45%) and 28 (36%) were found to carry Yr18 according to these markers, respectively.…”

“…Currently, there have been advances in development and mapping of molecular markers that are diagnostic for major Yr genes (Mago et al, 2002;Eriksen, 2004;Li and Niu, 2007;Bansal et al, 2009;Krattinger et al, 2009;Jia et al, 2011;Cabuk et al, 2011;Liu et al, 2014;Yaniv et al, 2015;Ahmad et al, 2015). These markers provide an important tool to plant breeders for marker assisted wheat breeding and also for pyramiding resistance genes in the absence of distinguishable rust virulences (Kaur et al, 2008).…”

Identification of stripe rust resistant genes in resistant synthetic hexaploid wheat accessions using linked markers

“…The use of fungicides has proved to be successful to reduce the damages to some extent; however, it has many side effects and not affordable by farmers of developing countries such as Pakistan (Chen, 2007). The most operative, cost-effective and eco-friendly technique is growing genetically resistant varieties (Cabuk et al , 2011). Genetic resistance has been proposed as pyramiding of resistant genes.…”