Assessment of Antibiotic Resistance Phenotype and Integrons in Salmonella enterica serovar Typhimurium Isolated from Swine

Abstract

ABSTRACT. Salmonella enterica serovar Typhimurium (S. Typhimurium) isolated and identified from swine were subjected for the analysis of antibiotic resistance pattern and clinically important class 1 and 2 integrons. In addition, S. Typhimurium isolates exhibiting ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, tetracycline and florfenicol (ACSSuTF) resistance pattern as described in most Salmonella enterica serotype Typhimurium definitive type 104 (DT104) were characterized by polymerase chain reaction. All the isolates were resistant to more than four antibiotics and showed the highest resistance to streptomycin (94.1%), followed by tetracycline (90.1%), ampicillin (64.7%), chloramphenicol (56.8%) and gentamicin (54.9%). MIC value for the ten isolates ranged between 0.125-2 µg/ml for ciprofloxacin. Among the beta-lactams used, only one of the isolate exhibited resistance to ceftiofur (MIC 8 µg/ml). Sixty eight percent of these multi drug resistance (MDR) S. Typhimurium isolates carried clinically important class 1 integron with 1kb (aadA) and/or 2kb (dhfrXII-orfF-aadA2) resistance gene cassettes. This study reports the increasing trend of multi drug resistance (MDR) S. Typhimurium with clinically important class 1 integron in pigs. In addition, emergence of the ACSSuTF-type resistance in S. Typhimurium PT other than DT104 may limit the use of resistance gene markers in its detection methods by PCR.

Antibiotic Resistance Patterns and Detection ofblaDHA-1inSalmonellaSpecies Isolates from Chicken Farms in South Korea

“…FJ424733) and OXA-F (5Ј-ATGAAAAACACAATACATATCAAC-3Ј and 5Ј-TTCCTGT AAGTGCGGACAC-3Ј; GenBank accession no. J02967) were as described in our previous studies (21,22). E. coli (E/123), K. pneumoniae (K/14), Salmonella enterica serovar Typhimurium DT104 (ATCC 2501) (21,22), and S. Montevideo (10) were used as the positive control.…”

“…J02967) were as described in our previous studies (21,22). E. coli (E/123), K. pneumoniae (K/14), Salmonella enterica serovar Typhimurium DT104 (ATCC 2501) (21,22), and S. Montevideo (10) were used as the positive control. Amplified PCR products of expected sizes were subjected to direct sequencing by an automatic sequencer and dye termination sequencing system (Macrogen Co., South Korea).…”

Diversity and antimicrobial resistance of Salmonella strains isolated from different sources in Burkina Faso

“…Salmonella Typhimurium was found in cattle, poultry, human and fish. S. Typhimurium has been implicated in many outbreaks worldwide (Rayamajhi et al, 2008). This serotype has a wellcharacterized ability to infect various species (Rabsch et al, 2002) and can survive for a long time in the environment (Baudart et al, 2000); these 2 factors enhance the ability of this serotype to be one of the most common causes of salmonellosis in many geographically diverse regions and has caused many disease outbreaks (Mather et al, 2013;Cui et al, 2008;Galanis et al, 2006).…”

First CTX-M type ß–lactamase-Producing and Ciprofloxacin Resistant Salmonella Infection Acquired by a Child in IRAN

“…Though non-typhoidal salmonellosis is self-limiting, but antibiotic therapy can be life saving in children, elderly, and immune compromised personals with systemic infections (1). Fluoroquinolones and thirdgeneration cephalosporins are major antibiotics used for treatment of invasive salmonellosis (2).…”