Assessment of biotoxicity of Cu nanoparticles with respect to probiotic strains of microorganisms and representatives of the normal flora of the intestine of broiler chickens

Abstract

Copper nanoparticle Cu (d = 55 ± 15 nm) and CuO nanoparticles (d = 90 ± 10 nm) were used in the studies (OOO Platina, Russia). Using the method of pure cultures, we extracted Lactobacillus, Enterococcus, and Enterobacterium from the intestines of broilers. Additionally, strains of Bacillus subtilis 10641 and Bifidobacterium were involved in probiotic strains. The data obtained in the course of the study testify to the insignificant biotoxicity of copper nanoparticles with respect to representatives of the genera Lactobacillus (30 to 15 μg/ml) and Bifidobacterium (30 μg/ml), with the most sensitive bacteria being the genus Lactobacillus, for which a concentration of 7.5 μg/ml was subinhibitory. The second stage was the study using method of agar wells. In the course of the experiment, we obtained results confirming the data of the research by the serial dilution method. In this case, as in the first case, the data indicate the insignificant biotoxicity of copper nanoparticles in relation to representatives of the genera Lactobacillus and Bifidobacterium. We have studied the bioaccumulating ability of microorganisms of the studied metals. In all the studies carried out, as in the first series of experiments, representatives of the genera Lactobacillus and Bifidobacterium with the lowest bioaccumulative ability were the most sensitive to copper nanoparticles and were 3.1 and 8.2%, respectively. The use of nanoparticles as a component of the fodder additive in small concentrations does not adversely affect not only the probiotic strains, but also the main representatives of the normoflora (Lactobacillus, Enterococcus, and Enterobacterium) of the poultry, the positive effect of the copper nanoparticles being directly related to low level of dissociation of nanoparticles, since biologically active ions will be released much more slowly, thereby creating a prolonged effect of exposure.

Evaluation of the impact of plant extracts in different concentrations on the ecosystem of broilers’ intestine

“…In groups I and II, representatives of class Clostridia decreased by 14.6-50.5% in comparison with the control. Earlier studies also demonstrated the positive effect of tannincontaining substances on inhibition of the development of microorganisms of class Clostridia [13], as well as the great variability in the development of micro-organisms in the small intestine of broilers [14]. Speaking of the cecal intestine, we recorded an increase of phylum Firmicutes due to representatives of classes Clostridia and Bacillus and the absence of representatives of phylum Bacteroidetes in the second group.…”

Influence of toxic metal exposure on the gut microbiota (Review)

“…In another study, in poultry, CuO NP added to the feed, did not affect probiotics or the main representatives of normal microbial community. The Cu active ions, due to the low dissociation degree from NP, are released more slowly and therefore have more prolonged positive effects (94).…”

ZnO and TiO2 nanoparticles alter the ability of Bacillus subtilis to fight against a stress

“…It is also used as a probiotic to promote the growth of broiler chicken or shrimp. Several studies have shown that the co-administration of nanoparticles and probiotics has no or few effect on the microbiome composition or activity [49,50]. But chronic oral absorption of nanoparticles, as a consequence of nanoparticles in food additives, disturbs the gut microbiome equilibrium and may be responsible for several chronic diseases [51].…”