Assessment of combination of pretreatment ofSorghum durrastalk and production of chimeric enzyme (β-glucosidase and endo β-1,4 glucanase,CtGH1-L1-CtGH5-F194A) and cellobiohydrolase (CtCBH5A) for saccharification to produce bioethanol

Assessment of indigenous fungal biocatalysts towards valorization of delignified physico‐chemically pretreated corn cobs and sugarcane bagasse

“…Mechanical pretreatments by milling, extrusion or comminution reduce the biomass particle size to ease handling and increase the surface to volume ratio. Physico‐chemical pretreatments integrate chemical (sodium hydroxide, potassium hydroxide, sulfuric acid, hydrochloric acid, liquid ammonia) and physical parameters (pressure, heat, microwave, sonication), thereby improving the accessibility of glycosidic linkages toward biomass deconstruction 7,9 . Several studies have reported the use of indigenous as well as commercial enzyme preparations (such as Primafast 200, CTec2, Multifect xylanase) after different pretreatments for enhanced enzymatic saccharification of biomass, yet none have been optimized for maximum efficiency at the commercial level 8,10,11 …”

“…Physico-chemical pretreatments integrate chemical (sodium hydroxide, potassium hydroxide, sulfuric acid, hydrochloric acid, liquid ammonia) and physical parameters (pressure, heat, microwave, sonication), thereby improving the accessibility of glycosidic linkages toward biomass deconstruction. 7,9 Several studies have reported the use of indigenous as well as commercial enzyme preparations (such as Primafast 200, CTec2, Multifect xylanase) after different pretreatments for enhanced enzymatic saccharification of biomass, yet none have been optimized for maximum efficiency at the commercial level. 8,10,11 Sugarcane bagasse (SB) and corn cobs (CC) contain 60-80% holocellulose, which makes them potential feedstocks for biorefineries as well as glycosyl hydrolase production.…”