Assessment of correlation between serum βTP and cystatin C as biomarkers for the detection of early nephropathy in Iraqi patients with type 2 diabetes

Abstract

Background: Diabetic nephropathy (DN) is one of the most well-known diabetic microvascular complications, affecting around 40% of individuals with type 2 diabetes mellitus (T2DM). It progresses to end-stage renal disease (ESRD), and its primary detection can be done via diabetes biomarkers. The power of early biomarker identification of DN in T2DM serum is evaluated in this study. Aim: This study aimed to assess the possibility of using ΒTP and Cys. C as earlier markers for the detection of nephropathy in patients with type 2 DM. Materials and Methods: This is a cross-sectional study. It included one hundred twenty patients with T2DM, composed of 66 males and 45 females, and aged 40–69 years, who were divided into 3 groups by using the urinary albumin/creatinine ratio (ACR): Group I: (N = 40 Normoalbuminuria UACR < 30 mg/g as control), Group II: (N = 40 Microalbuminuria UACR 30–300 mg/g), Group III: (N = 40 Macroalbuminuria UACR ˃ 300 mg/g). In all groups, βTP and Cys. C were estimated in the serum of patients, and both biomarkers had the same methodology by quantitative enzyme immunoassay (double-antibody sandwich).

Role of Beta Trace Protein and Cystatin C as a Potential Biomarker for Early Detection of Type 2 Diabetic Nephropathy

“…Patients and healthy control: included 120 subjects from both sexes (female and male), which is the into four groups according‫ا‬ to their (UACR‫. )ا‬ the clinical‫ا‬ characteristics of the four study groups statistically significant difference was shown in the table (2). Participant were divided‫ا‬ into four groups according‫ا‬ to Albumin Creatinine Ratio (ACR) and HbA1c, the mean ± SD difference of S. Cys C and S. BTP between the 4 groups was assessed using ANOVA test.…”

“…BTP in compare to microalbuminuria and normoalbuminuria all in compare to healthy control , as presented in table (2). Using the Pearson correlation coefficient, the relationship between S. BTP and S. Cys C. in the three diabetics groups was evaluated.…”

“…The serum Beta Trace Protein (BTP) level as shown in the table (2), where high in the macroalbumin group, compared with the other groups, microalbumin, normalbumin and healthy control, P value (0.001).…”

“…The role of both Serum Beta Trace Protein and Cystatin C as early diagnostic markers for Diabetic Nephropathy was studied. In this study, laboratory chemical measurements were made, all of which are shown in table (2).…”

“…One of the most prevalent microvascular issues of diabetes is diabetic nephropathy (DN), which affects 40 percent of individuals with type 2 diabetes. Diabetes biomarkers can be utilized to detect it before it progresses into endstage renal disease (ESRD) (2).…”