Assessment of Genetic Diversity among Musa Cultivars based on Sequence-Related Amplified Polymorphism Technique

Morphological and Molecular Characterizations of Musa (ABB) ‘Mali-Ong’ in Thailand

“…The intensity of the ultraviolet fluorescent bands was proportional to the amount of DNA. The intensity was compared with a known standard DNA ladder of 100 bp (ONEMARK 100 DNA bp ladder, Bio-Helix Co. Ltd., New Taipei City, Taiwan) [ 13 ].…”

“…A thermal cycler was used to perform DNA amplification (T100 TM , Bio-Rad, Hercules, CA, USA). An initial denaturation at 94 °C for 5 min was followed by 35 cycles of denaturation for 30 s at 94 °C, annealing for 1 min at 50–53 °C, extension for 1 min at 72 °C, and a final extension for 2 min at 72 °C [ 13 ].…”

“…A total of 20 µL of 2× OnePCR TM Plus (Bio-Helix Co., Ltd., New Taipei City, Taiwan), including Taq DNA polymerase, dNTP mix and gel loading dye, 5 M SRAP primers, and 50 ng of genomic DNA, was used for the PCR experiments. Thermal cycler amplifications were carried out using a denaturation step at 94 °C for 3 min, followed by 5 cycles of 3 steps: 1 min for denaturation at 94 °C, 1 min for annealing at 35 °C, and 2 min for an extension at 72 °C, followed by 35 cycles with an annealing temperature of 50 °C and a final prolongation step for 5 min at 72 °C [ 13 ].…”

“…Molecular markers, including random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), simple sequence repeat (ISSR), and sequence-related amplified polymorphism (SRAP) markers, have been employed to examine genetic diversity among banana accessions and species [ 2 , 10 , 11 , 12 ]. SRAP markers have been used to determine the relationship of a cross between Musa (ABB) and two wild cultivars (AA and BB) [ 13 ]. However, no precise information exists on the morphological traits of ‘Kluai Nam Wa Mali-Ong’.…”

Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers

“…Understanding the diversity and relationships of genetic resources are required as a foundation for the development and sustainability of cultivated production, and the evaluation of genetic diversity provides effective information to maintain and manipulate genetic resources. Presently, the ability to evaluate genetic diversity and the relationships within and between populations using molecular markers has resulted from the rapid development of molecular genetics, and molecular markers have been extensively applied in the assessment of genetic diversity, particularly in Musa cultivars (Rotchanapreeda et al 2016;Swain et al 2016;Chang et al 2017;Silva et al 2017;Arora et al 2018;Bawin et al 2019;Santos et al 2019;Marimuthu et al 2019;Wanvisait et al 2019;Boonsrangsom et al 2020;Saraswathi et al 2020;Wahyudi and Rifliyah 2020;Carracedo et al 2021, González et al 2021Mertens et al 2021). Recently, many new marker techniques have been developed in line with the rapid growth of Musa genomic research (Wahyudi et al 2022;Dehery et al 2022;Premjet et al 2022).…”