ASSESSMENT OF GENETIC DIVERSITY AMONG HEADED CABBAGE(Brassica oleracea L.) ACCESSIONS BY USING SSR MARKERS

Домблидес, А. С.

doi:10.15389/agrobiology.2020.5.890eng

S-h. biol.

2020

DOI: 10.15389/agrobiology.2020.5.890eng

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ASSESSMENT OF GENETIC DIVERSITY AMONG HEADED CABBAGE(Brassica oleracea L.) ACCESSIONS BY USING SSR MARKERS

А. С. Домблидес¹

Abstract: Из всех разновидностей капусты в Российской Федерации наиболее распространены традиционные сорта и гибриды капусты кочанной Brassica oleracea L. сonvar. capitata (L.). Классификация селекционного материала с использованием ДНК маркеров позволяет выделить ценные генотипы и установить между ними генетические взаимосвязи для последующей селекции на генетически отличимые формы. Микросателлитные маркеры (simple sequence repeats, SSR) широко используют для генетической идентификации и генотипирования сельскохозяйств… Show more

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Cited by 2 publications

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Results and prospects for the development of scientific research in FSBSI FSVC

et al. 2021

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The article reflects the issues of the current state of scientific research at the FSBSI FSVC, shows the directions and prospects for the development of selection and seed production of vegetable crops. The main mission of the selection and seed-growing work of the FSVC is the creation and reproduction of varieties and hybrids of vegetable and melon crops of a new generation, characterized by resistance to abiotic and biotic stressors, high productivity and product quality. One of the main ways to increase the efficiency of the breeding process is the use of modern methods of biotechnology and molecular genetics, which make it possible to obtain new genotypes and reduce the time of breeding. Success has been achieved in the creation of homozygous lines of cabbage, squash, cucumber, carrots. For the first time, it was possible to complete a full cycle of obtaining doubled radish haploids in microspore culture in vitro. The institution pays special attention to immunological research on the resistance of crops and new varieties to diseases, as well as protecting plants from pests and diseases. Phytosanitary monitoring, assessment and selection of resistant genotypes are carried every years. One of the important theoretical and practical problems solved at the institution is the development of environmentally friendly products based on preparations obtained from plants with a high content of biologically active compounds with adaptogenic properties. In the center, a new direction of research in traditional breeding has received significant development - the creation of varieties of vegetable plants with an increased content of biologically active substances and antioxidants, on the basis of which functional products are created. The persistence of modern varieties and hybrids of table carrots, depending on the biochemical composition, has been studied. Methods for enriching vegetable plants with micronutrients are being developed. A large amount of scientific research is being carried out on the development of technologies for the cultivation of new varieties and hybrids, as well as technologies for their seed production. At the branches located in different soil and climatic conditions, hospitals were established to study the interaction of factors controlling soil fertility and the productivity of vegetable crops in agrocenoses (crop rotation, soil treatment systems, fertilizers and plant protection). In general, at the FSBSI FSVC, all research is aimed at developing and improving methods in breeding and seed production of vegetable crops, creating new breeding achievements and developing varietal technologies for their cultivation.

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Results and prospects for the development of scientific research in FSBSI FSVC

et al. 2021

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Modern trends in the development of selection of vegetable and melon crops

et al. 2022

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The article reflects the issues of the current state of scientific research in the FSBSI FSVC, directions and prospects for the development of selection and seed production of vegetable crops are shown. Varieties have been created that realize the bioclimatic potential of the cultivation zone, meet market requirements, and are capable of producing high yields under the influence of biotic and abiotic stressors. Selection is carried out in a combination of classical methods with innovative technologies.Biotechnologists have improved the basic protocol for in vitro microspore culture for the Brassicaceae, stages of technology for obtaining doubled haploids in microspore culture in vitro for the Apiaceae are being developed. For the first time, an effective protocol for obtaining double haploids of European radish has been developed. Doubled haploids of vegetable crops of the Cucurbitaceae, Amarantháceae, Amaryllidáceae were obtained in vitro in the culture of unpollinated ovules. The Center conducts research on the increased content of biologically active substances and antioxidants. An important place is occupied by immunological studies on the resistance of vegetable crops to harmful diseases. The institution develops systems of protective measures against pests, diseases and weeds for vegetable crops based on the use of new environmentally friendly preparations. On the basis of fundamental and priority applied research of previous years, the creation of 21 varieties and hybrids of cabbage, table beet, tomato, sweet pepper, onion, chives, leek, cucumber, melon, lettuce, chervil, potato, aster, lychnis has been completed. Along with the creation of new breeding achievements, technologies for their cultivation are being improved through the use of new agricultural practices, microfertilizers, biological products and humates on vegetable crops in various soil and climatic zones of Russia. New agricultural methods are being developed in the cultivation of mushrooms. In the field of seed production, methods are being developed to increase the germination of seeds, seed production, elements of the technology for the production of mother liquors and seeds of various vegetable and melon crops; zonal seed production technologies that increase their yield. In 2021, a team of scientists received an award from the Government of the Russian Federation for scientific work "Development and implementation of innovative technologies for growing vegetables and potatoes to provide the population with environmentally friendly food".

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ASSESSMENT OF GENETIC DIVERSITY AMONG HEADED CABBAGE(Brassica oleracea L.) ACCESSIONS BY USING SSR MARKERS

Cited by 2 publications

References 18 publications

Results and prospects for the development of scientific research in FSBSI FSVC

Results and prospects for the development of scientific research in FSBSI FSVC

Modern trends in the development of selection of vegetable and melon crops

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