Assessment of genetic diversity of landraces of Dendrocalamus hamiltonii using AFLP markers and association with biochemical traits

Abstract

ABSTRACT. Fermented bamboo shoots are popular traditional food items of various ethnic groups of the northeastern India, especially in Manipur State. Dendrocalamus hamiltonii is an economically important bamboo species used to produce fermented bamboo shoots. We studied genetic variability of this bamboo species in Chandel and Imphal-East (commercial production districts), using AFLP molecular markers. Each of the selected primers detected polymorphisms and 1614 (95.8%) were found to be polymorphic. Cluster analysis based on Dice similarity coefficients using UPGMA differentiated the populations into two major groups. Principal coordinate analysis based on the AFLP data clearly separated the populations according to their genetic diversity and antioxidant activity. Four primers were tested through multiple regression analysis to identify marker-trait association between AFLP data and biochemical attributes, i.e., antioxidant activity and total cyanide content. The 273 bp generated by EcoRI-AAG(Joe)/MseI-CTC showed high positive correlation with antioxidant activity (r = 0.729, P < 0.01). The 396 bp generated by EcoRI-AAC(Ned)/MseI-CTG were negatively correlated with cyanide content (r = -0.694, P < 0.01). Thus, we found association of DNA markers with antioxidant activities and total cyanide content. These results could be of use for the identification of superior genotypes with desirable traits.

Grappling the High Altitude for Safe Edible Bamboo Shoots with Rich Nutritional Attributes and Escaping Cyanogenic Toxicity

“…Irrespective of toxicity, some young edible bamboo shoots (≤30 days) possess enormous nutritious potentials such as high fibre content with antioxidant and antitumor properties [5, 6]. Consequently, the demand for bamboo shoots is high and farmers are often challenged to match supply in both quality and quantity.…”

“…Biochemical studies on antioxidant activity, cyanogenic glycoside content, and nutrient content have been reported for a few species [5, 7–9], without correlation to the altitudes of sample collection, equally lacking morphological and molecular characterization. Based on previous work, it is difficult to sort out edible bamboo species, requiring low-processing capital inputs to eliminate the poisonous components.…”

Molecular identification of bamboo genera and species based on RAPD-RFLP markers

“…were converted to SCAR (Sequence Characterized Arbitrary Regions) in order to obtain proper markers for the molecular identification of the species (Das et al 2005). Another class of markers, the AFLP (Amplified Fragment Length Polymorphism), were screened for detecting genetic variability and defining phylogenetic relationships among bamboo species from a collection of Singapore (Loh et al 2000) and India (Waikhom et al 2012). As alternative dominant genetic markers, ISSR (Inter Simple Sequence Repeats) markers have been used to distinguishing bamboo species (Lin et al 2010;Mukcherjee et al 2010).…”

Black List of Unreported Pathogenic Bambusicolous Fungi Limiting the Production of Edible Bamboo

“…Young succulent bamboo shoots of D. hamiltonii are consumed fresh or fermented as vegetable; and preferred over other species because its fermented products retained good taste and low water content [13]. At present, there is no report on the diversity of pathogenic bambusicolous fungi of any edible bamboo species.…”