Assessment of Heat Tolerance in Bread Wheat Using Some Agronomic Traits and SRAP Markers = تقييم التحمل للحرارة في قمح الخبز باستخدام بعض الصفات المحصولية و واسمات SRAP

Abstract

Fifty bread wheat lines were evaluated for heat tolerance and compared to some local cultivars under three sowing dates (November 25th, December 15th and January 5th). Four agronomic traits were evaluated, i.e. No. of spikes per plant, grain yield per plant, 100-kernel weight and harvest index under normal and stress conditions. Analysis of variance showed highly significant variations among the tested lines and demonstrating that the main effect of sowing dates was due to the late date. Grain yield per plant was the most affected trait by heat followed by 100-kernel weight and No. of spikes per plant, while harvest index showed the lowest reduction due to heat stress. Six lines (L1, L11, L16, L34, L37 and L41) showed heat tolerance based on high performance in grain yield/plant by 29.45, 29.75, 27.75, 27.43, 27.37 and 31.90 g, respectively under late sowing conditions as well as low heat sensitivity index. The sequence related amplified polymorphism (SRAP) was able to differentiate between bulked DNA samples of lines with the highest and lowest performance in agronomic traits under heat stress. SRAP generated 2, 1 and 3 bands specific for lines with high performance of No. of spikes per plant, grain yield per plant and harvest index, respectively as well as it showed 5 and 3 bands specific for lines with low performance of grain yield per plant and harvest index, respectively. These specific bands could serve in wheat genotyping and screening, and might be used as SRAP markers associated with heat tolerance in wheat breeding programs.

Phenotypic and genotypic diversity analysis of some Egyptian barley cultivars (Hordeum vulgare L.) under different heat stress conditions

“…High polymorphic rate and PIC value suggested a high level of heterogeneity among studied barley cultivars. These results agree with those obtained (Said et al, 2015, Mariey et al, 2018, 2021. Since SRAP marker to evaluate the genetic variations among barley and they suggested that SRAP technology is useful for genetic diversity and relationship analyses, marker assisted selection and genetic map construction in barley.…”

“…Accordingly, it is confirmed that the SRAP marker could be efficiently used to assess genetic variation among Egyptian barley and their ability for tolerance to heat stress, which the dendrogram of SRAP markers had clustered all the Egyptian cultivars in to two groups each group include the most closed cultivars together according their response to heat stress. (Said et al, 2015 andAnwer et al, 2021).…”

“…SRAP markers were successfully used in determining the genetic diversity and relationships among in crops such as barley for abiotic stress such as salinity and drought (Ahmed et al, 2021;Mariey, 2018;2021. For heat stress, SRAP also have been successfully used to measuring the genetic diversity and relationships in many cereal crops such as wheat (Said et al, 2015) and maize (Anwer et al, 2021). Until now, there is no many reports on determining the genetic diversity of Egyptian barley cultivars and or genotypes by SRAP markers for heat stress.…”

Development of SCAR markers related to heat tolerance in Kentucky bluegrass

“…HT identification is the most basic, critical and difficult part in the HT researches. In some studies, agronomic traits or physiological and biochemical indicators related to HT were used as criteria for HT identification (Said et al, 2015;El-Rawy and Youssef, 2014;Sun et al, 2015a;Jespersen et al, 2018;Priya et al, 2018). In this study, plant appearance (yellow leaves) was taken as the only criterion to evaluate the HT of plants, which could more directly reflect the stress degree of plants and facilitate large-scale identification.…”

“…For example, in wheat (Triticum aestivum), Moustafa et al (2014) identified SRAP markers linked with six agronomic traits under drought stress. Said et al (2015) detected SRAP markers associated with four agronomic traits related to HT. El-Rawy and Youssef (2014) evaluated the drought and HT in wheat based on seedling traits, and screened the SRAP markers associated with them.…”