Assessment of micronuclei induction in peripheral blood and gill cells of some fish species from Aliağa Bay Turkey

Assessment of toxicity in fresh water fish Labeo rohita treated with silver nanoparticles

“…In fish, the micronuclei test of blood erythrocytes is usually preferred because the fish erythrocytes are nucleated in nature. However, the gills and kidney tissues can also be used (Arslan et al 2015;Bolognesi and Hayashi 2011;Palhares and Grisolia 2002). In the present study, maximum frequency of MN (5.03 ± 1.89%) was at -1 Ag-NPs concentration after 14 and continues to increase.…”

Piscine micronucleus assay and the evidence of environmental degradation: the case of catfish from Brazilian tropical estuaries / Bioensaio de micronúcleo em peixes e evidências de degradação ambientai: o caso dos bagres de estuários tropicais brasileiros

“…The extremely low rates of nuclear morphologies alterations observed in this study were not expected because previous researches in the same estuaries revealed increasing environmental degradation (Santana et al 2015) and other studies demonstrated that fish from polluted areas exhibit significantly higher rates of MN and NA than non-polluted sites (Bombail et al 2001, Çavas & Ergene-Gözükara 2005, Amado et al 2006, Kirschbaum et al 2009, Rybakovas et al 2009, Osman et al 2011, Arslan et al 2015, Fatima et al 2015. In Turkey, five species of fish collected at Aliaga Bay presented from 23 -53.33 ‰ MN and 4 -32.7 ‰ NA in the polluted area, compared to 11 -18 ‰ MN and 1-5 ‰ NA in the clean area (Arslan et al 2015). However, Carrasco et al (1990) did not find significant differences between NA rates in fish from polluted and clean sites, as occurred in Ceará estuaries.…”

“…Micronuclei (MN) may form from chromosomal fragmentation or separation of entire chromosomes during the anaphase (Al-Sabti & Metcalfe 1995); they also can be induced by apoptosis or actions of physical agents and pollutants that interact with DNA (Bombail et al 2001). High frequency of MN in organisms from polluted sites has been reported by several authors and it has been described as sensitive biodetector of genotoxicants, in studies involving chronic exposure to different types of environmental pollutants with clastogenic and aneugenic properties, providing early warning of eco-damage and stress on the health of organisms and ecosystems (Rodriguez-Cea et al 2003, Udroiu 2006, Ergene et al 2007, Monserrat et al 2007, Ahmed et al 2013, Arslan et al 2015, Perez-Coyotl et al 2017, Farag & Alagawany 2018, Hussain et al 2018. Other non-specific Nuclear Abnormalities (NA) tend to occur prior the MN formation, and although NA is not totally considered as a genotoxic or mutagenic damage as their origin is not well described as MN, it is still used as a complement to MN frequency, then both together can indicate citogenotoxicity (Ayllon & Garcia-Vazquez 2000, Kirschbaum et al 2009, Seriani et al 2011.…”

Micronucleus formation in mussel’ (Mytilus galloprovincialis, Lamarck 1819) haemolymph, liver, and gill cells as a biomarker in the assessment of genotoxicity in İzmir Bay (Aegean Sea, Turkey)

“…It is not possible, not economic, and time-consuming to determine the concentration of such substances in the tissues analytically with available chemical methods. Thus, the biological methods and those based on screening for carcinogenic and mutagenic substances in the tissues of indicator organisms have gained importance (Arslan et al, 2010;2015). Genotoxic substances show the initial effect and even if the agent disappears, the effect continues.…”

“…Micronuclei (MN) test is one of the most reliable techniques used to determine genetic changes in the organisms in contaminated waters and complex mixtures. In recent years, this test has been improved using many aquatic organisms (Hayashi et al, 1998, Arslan et al, 20102015;Dailianis et al, 2003, Tsarpali andDailianis, 2012). The MN assay is today applied in laboratory and field studies using hemocytes and gill cells from bivalves, mainly from the genera Mytilus.…”