Assessment of microvascular endothelial function in type 1 diabetes using laser speckle contrast imaging

Matheus, Alessandra Saldanha de Mattos; Clemente, Eliete L.S.; Rodrigues, Maria de Lourdes Guimarães; Valença, D.; Gomes, Marı́lia B.

doi:10.1016/j.jdiacomp.2016.12.007

Cited by 28 publications

(22 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…112,113 Also a study was published about the assessment of the forearm endothelium showing significantly impaired vasodilatory response with type I diabetes. 114 Finally, dual-wavelength LSCI allows for simultaneous imaging of blood flow and the oxyhemoglobin and deoxyhemoglobin concentrations. 115,116 4 Current Limitations of LSCI…”

Section: Other Uses Of Lscimentioning

confidence: 99%

Clinical applications of laser speckle contrast imaging: a review

et al. 2019

View full text Add to dashboard Cite

When a biological tissue is illuminated with coherent light, an interference pattern will be formed at the detector, the so-called speckle pattern. Laser speckle contrast imaging (LSCI) is a technique based on the dynamic change in this backscattered light as a result of interaction with red blood cells. It can be used to visualize perfusion in various tissues and, even though this technique has been extensively described in the literature, the actual clinical implementation lags behind. We provide an overview of LSCI as a tool to image tissue perfusion. We present a brief introduction to the theory, review clinical studies from various medical fields, and discuss current limitations impeding clinical acceptance.

show abstract

Section: Other Uses Of Lscimentioning

confidence: 99%

Clinical applications of laser speckle contrast imaging: a review

et al. 2019

View full text Add to dashboard Cite

show abstract

“…A study was published regarding the evaluation of the forearm endothelium presenting notably impaired vasodilatory response with type I diabetic patient. 43…”

Section: Other Application Of Lscimentioning

confidence: 99%

A review of the clinical applications and performance of laser speckle contrast imaging

et al. 2020

View full text Add to dashboard Cite

<p class="abstract">Laser speckle contrast imaging (LSCI) is a useful tool for visualizing full-field blood flow images. Speckle pattern is formed when a coherent light illuminates a rough object, and the backscattered radiation is transformed into images and be shown on a screen. Movement within the object results in the fluctuation of patterns over time. The same data can be obtained by employing the Doppler effect, yet producing a two-dimensional Doppler map needs scanning; speckle imaging renders the same information without the requirement to scan. Nowadays, LSCI has gained expanded consideration, in part because of its accelerated adoption for blood flow studies in the different surgical departments. Here we represent and review the application of laser speckle contrast methods to the field of perfusion visualization as clinical studies from various medical fields and discuss the limitations hindering clinical acceptance.</p>

show abstract

“…Other possible applications for handheld LSI in a clinical setting include blood flow imaging of the extremities for patients with diabetes 19 or monitoring of peripheral vascular disease. 20 A need also exists for noninvasive blood flow measurements of preterm neonates with potentially abnormal blood flow due to developmental issues.…”

Section: Journal Of Biomedicalmentioning

confidence: 99%

Correcting for motion artifact in handheld laser speckle images

et al. 2018

View full text Add to dashboard Cite

Laser speckle imaging (LSI) is a wide-field optical technique that enables superficial blood flow quantification. LSI is normally performed in a mounted configuration to decrease the likelihood of motion artifact. However, mounted LSI systems are cumbersome and difficult to transport quickly in a clinical setting for which portability is essential in providing bedside patient care. To address this issue, we created a handheld LSI device using scientific grade components. To account for motion artifact of the LSI device used in a handheld setup, we incorporated a fiducial marker (FM) into our imaging protocol and determined the difference between highest and lowest speckle contrast values for the FM within each data set (Kbest and Kworst). The difference between Kbest and Kworst in mounted and handheld setups was 8% and 52%, respectively, thereby reinforcing the need for motion artifact quantification. When using a threshold FM speckle contrast value (KFM) to identify a subset of images with an acceptable level of motion artifact, mounted and handheld LSI measurements of speckle contrast of a flow region (KFLOW) in in vitro flow phantom experiments differed by 8%. Without the use of the FM, mounted and handheld KFLOW values differed by 20%. To further validate our handheld LSI device, we compared mounted and handheld data from an in vivo porcine burn model of superficial and full thickness burns. The speckle contrast within the burn region (KBURN) of the mounted and handheld LSI data differed by <4 % when accounting for motion artifact using the FM, which is less than the speckle contrast difference between superficial and full thickness burns. Collectively, our results suggest the potential of handheld LSI with an FM as a suitable alternative to mounted LSI, especially in challenging clinical settings with space limitations such as the intensive care unit.

show abstract

Assessment of microvascular endothelial function in type 1 diabetes using laser speckle contrast imaging

Cited by 28 publications

References 19 publications

Clinical applications of laser speckle contrast imaging: a review

Clinical applications of laser speckle contrast imaging: a review

A review of the clinical applications and performance of laser speckle contrast imaging

Correcting for motion artifact in handheld laser speckle images

Contact Info

Product

Resources

About