Assessment of the antioxidant and enzyme inhibition activities of <i>Cousinia iconica</i> with focus on phytochemical investigation by LC-MS/MS

Paşayeva, Leyla; Eruygur, Nuraniye; Ayaz, Fatma; Tuğay, Osman; Fatullayev, Hanifa

doi:10.1080/11263504.2020.1829736

Cited by 11 publications

(7 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CIE also showed high activity on αamylase with 864.57 ± 0.68 µg/mL IC50 values. In the same study, rutin and cynarin were determined as the main constituents of CIM and CIE [18]. These findings supported our results.…”

Section: Lc-ms/ms Analyses Of Bioactive Compoundssupporting

confidence: 88%

“…The mass spectra revealed the presence of apigenin [17], caffeic acid-3-glucoside, caffeic acid derivative-I, [18], chlorogenic acid, isorhamnetin 3-O-rutinoside [17], kaempferol-3-O-glucoside [19], malic acid [20], quinic acid [21], rutin [22], cynarin, isoquercitrin [18] and others. The total ion chromatogram and mass spectra of the sub-extract are shown in Figures 1 and 2.…”

Section: Lc-ms/ms Analyses Of Bioactive Compoundsmentioning

confidence: 99%

See 1 more Smart Citation

LC–ESI-MS/MS-BASED PHYTOCHEMICAL PROFILE, ANTIOXIDANT AND ENZYME INHIBITION ACTIVITIES OF EXTRACTS FROM COUSINIA AINTABESIS BOISS. & HAUSSKN

YILMAZ¹

2022

FARMACIA

View full text Add to dashboard Cite

This study aimed to evaluate the antioxidant activity, the inhibitory effect on digestive enzymes linked to diabetes and the phytochemical composition of an active sub-extract obtained from Cousinia aintabensis (Asteraceae). The antioxidant effect of extracts was investigated with DPPH • , ABTS •+ and FRAP tests and the inhibition effect was tested on α-amylase and αglucosidase. The quantitative and qualitative determination of the compounds within the active extract was carried out by LC-MS/MS. The highest total phenolic (185.816 ± 0.003 mg GAE/g extract) and total flavonoid content (147.423 ± 0.003 mg CA/g extract) was found in ethyl acetate sub-extract of C. aintabensis (CAE). It also showed the highest antioxidant activity on DPPH • (IC50 = 0.473 ± 0.056 mg/mL), ABTS •+ (at a conc. of 0.5 mg/mL equivalent to 0.543 ± 0.005 µM Trolox) and FRAP (at a conc. of 1 mg/mL equivalent to 2586 ± 6.421 mmol Fe 2+ ) tests. Furthermore, the highest α-amylase and α-glucosidase activity were also found in CAE (IC50 = 0.361 ± 0.010 µg/mL for α-glucosidase and 1.010 ± 0.010 for α-amylase). According to LC-MS/MS analysis, the main compounds of CAE were cynarin and isorhamnetin 3-O-rutinoside. RezumatAcest studiu și-a propus evaluarea activității antioxidante, a efectului inhibitor asupra enzimelor digestive implicate în diabet precum și a compoziției fitochimice a unui subextract activ obținut din Cousinia aintabensis (Asteraceae). Efectul antioxidant a fost investigat cu ajutorul testelor DPPH • , ABTS •+ și FRAP. Efectul inhibitor a fost testat asupra α-amilazei și α-glucozidazei. Determinarea cantitativă și calitativă a compușilor a fost efectuată prin LC-MS/MS. Cel mai mare conținut total de fenoli (185,816 ± 0,003 mg GAE/g extract) și de flavonoide (147,423 ± 0,003 mg CA/g extract) a fost găsit în subextractul de acetat de etil (CAE). De asemenea, acesta a arătat cea mai mare activitate antioxidantă asupra DPPH • (IC50 = 0,473 ± 0,056 mg/mL), ABTS •+ (la o concentrație de 0,5 mg/mL echivalent cu 0,543 ± 0,005 µM Trolox) și FRAP (la o concentrație de 1 mg/mL echivalent cu 2586 ± 6,421 mmol Fe 2+ ). CAE a inhibat activitatea α-amilazei și α-glucozidazei (IC50 = 0,361 ± 0,010 µg/mL pentru α-glucozidază și 1,010 ± 0,010 pentru α-amilază). Conform analizei LC-MS/MS, principalii compuși ai subextractului au fost cinarina și izoramnetina 3-O-rutinozida.

show abstract

Section: Lc-ms/ms Analyses Of Bioactive Compoundssupporting

confidence: 88%

Section: Lc-ms/ms Analyses Of Bioactive Compoundsmentioning

confidence: 99%

LC–ESI-MS/MS-BASED PHYTOCHEMICAL PROFILE, ANTIOXIDANT AND ENZYME INHIBITION ACTIVITIES OF EXTRACTS FROM COUSINIA AINTABESIS BOISS. & HAUSSKN

YILMAZ¹

2022

FARMACIA

View full text Add to dashboard Cite

show abstract

“…The identification of the compounds in the active TFE sub-extract was carried out by registered mass spectra fragmentation patterns, the NIST (National Institute of Standards and Technology) mass spectral database (version 2.3, Gaithersburg, MD, USA) and using literature data. According to the results, patuletin, patuletin 3,7-diglucoside [ 7 ], naringin [ 27 ] and 1,4-dicaffeoylquinic acid [ 8 , 28 ] were detected in the active sub-extract ( Table 5 and Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Chemical Composition, Antioxidant and Antidiabetic Activity of Rhaponticoides iconiensis Flowers: Effects on Key Enzymes Linked to Type 2 Diabetes In Vitro, In Silico and on Alloxan-Induced Diabetic Rats In Vivo

et al. 2022

View full text Add to dashboard Cite

Diabetes mellitus (DM) is one of the globally worst killer diseases. In this study, the in vitro and in vivo antidiabetic activity and antioxidant capacity were determined and the phytochemical analyses were carried out on flower extract and sub-extracts of Rhaponticoides iconiensis. The in vitro antidiabetic activity was tested with α-amylase and α-glucosidase enzyme inhibition methods and an in vivo OGTT test in healthy and alloxan-induced rats. Although, the antioxidant activity was investigated with DPPH●, ABTS●+ and FRAP tests, the phytochemical composition analysis was carried out by LC-MS/MS. The highest α-glucosidase and α-amylase activity even from positive control acarbose were found in the ethyl acetate sub-extract of R. iconiensis (IC50 = 11.737 ± 0.823 µg/mL and 84.247 ± 0.721 µg/mL, respectively). This sub-extract also was active according to the results of in vivo tests. Moreover, the highest antioxidant activity on DPPH● (IC50 = 0.126 ± 0.002 mg/mL), FRAP (at a concentration of 1 mg/mL equivalent to 3112.052 ± 2.023 mmol Fe2+) and ABTS+● (at a concentration of 0.5 mg/mL equivalent to 0.608 ± 0.005 µM Trolox) tests. In addition, LC-MS/MS analyses of the active sub-extract revealed mainly the presence of patuletin, patuletin 3,7-diglucoside, naringin and 3,4-dicaffeoylquinic acid detected in the active sub-extract. In silico molecular docking and dynamics simulations studies were performed on these compounds with α-amylase and α-glucosidase enzymes for protein–ligand interactions and stability.

show abstract

“…This test was carried out in accordance with the procedure by Paşayeva et al (18). For 14 minutes, a mixture of 20 μl of extract sample/positive control at various concentrations, 20 μl of enzyme (0.22 U/ml for AChE, and0.1 U/ml for BuChE) (Sigma-Aldrich), and 140 μl of 0.1 mM phosphate buffer (pH: 6.8) were incubated with 140 μl of 0.1 mM phosphate buffer (pH: 6.8).…”

Section: Acetylcholinesterase (Ache)/butyrylcholinesterase (Buche) In...mentioning

confidence: 99%

Biological activities of three Phlomis species

Eruygur¹,

Kırcı²,

Ayaz³

et al. 2022

jrp

Self Cite

View full text Add to dashboard Cite

The genus Phlomis, which belongs to the Lamiaceae family, comprises 100 species native to Turkey, Asia, Europe, and North Africa. As reported in many studies, Phlomis genus has unique therapeutic and aromatic properties. According to the data obtained from traditional uses, Phlomis species have a characteristic taste and so are traditionally consumed as herbal tea to treat diabetes, gastrointestinal disease as well as maintain overall health by protecting the liver, kidney, bone, and cardiovascular systems. In this present work, it was focused on the evaluation of the in-vitro enzyme inhibition (α-glucosidase, α-amylase, acetylcholinesterase (AChE)/butyrylcholinesterase (BuChE), and tyrosinase (TYR) and non-enzyme antioxidant activities (TPC, TFC, DPPH, and ABTS radical scavenging assays, and Iron-chelating activity assay) of the dichloromethane (DCM) and methanol extracts prepared from aerial parts of Phlomis nissolii, P. samia and P. sieheana with ultrasonic assisted method. All methanol extracts were rich in phenolic compounds and have high antioxidant activities. In the literature, there is no activity study related to the extracts of P. sieheana. The methanol extract of P. samia was the most active among other observed species. Especially the high tyrosinase enzyme inhibition activity of P. sieheana DCM extract may be promising for cosmetic product development.

show abstract

Assessment of the antioxidant and enzyme inhibition activities of Cousinia iconica with focus on phytochemical investigation by LC-MS/MS

Cited by 11 publications

References 42 publications

LC–ESI-MS/MS-BASED PHYTOCHEMICAL PROFILE, ANTIOXIDANT AND ENZYME INHIBITION ACTIVITIES OF EXTRACTS FROM COUSINIA AINTABESIS BOISS. & HAUSSKN

LC–ESI-MS/MS-BASED PHYTOCHEMICAL PROFILE, ANTIOXIDANT AND ENZYME INHIBITION ACTIVITIES OF EXTRACTS FROM COUSINIA AINTABESIS BOISS. & HAUSSKN

Evaluation of the Chemical Composition, Antioxidant and Antidiabetic Activity of Rhaponticoides iconiensis Flowers: Effects on Key Enzymes Linked to Type 2 Diabetes In Vitro, In Silico and on Alloxan-Induced Diabetic Rats In Vivo

Biological activities of three Phlomis species

Contact Info

Product

Resources

About