Assessment of three SERS approaches for studying E. Coli O157:H7 susceptibility to ampicillin

Gukowsky, Joshua C.; Yang, Tianxi; He, Lili

doi:10.1016/j.saa.2021.120239

Cited by 8 publications

(10 citation statements)

References 36 publications

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“…SERS has been successfully applied to analyze the different responses of antibiotic resistant and sensitive bacteria during exposure to antibiotics to assess the antibiotic susceptibility of bacteria. 80 It has been applied to differentiate the antibiotic-resistant and susceptible E. coli strains, [81][82][83] S. Typhimurium, 84 B. cereus, 82,85 S. aureus, 86 and carbapenem sensitive and resistant Klebsiella strains from clinical samples. 87 In addition to the instant one-time treatment of antibiotics, the antibiotic susceptibility testing was conducted over a long period such as three months, or 21 days in some studies, to investigate the dynamic molecular changes in the drug-resistant strains.…”

Section: Different Bacterial Responses During Antibiotics Exposurementioning

confidence: 99%

Recent advances of Raman spectroscopy for the analysis of bacteria

Rodriguez

Zhang

Wang

2023

Analytical Science Advances

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Rapid and sensitive bacteria detection and identification are becoming increasingly important for a wide range of areas including the control of food safety, the prevention of infectious diseases, and environmental monitoring. Raman spectroscopy is an emerging technology which provides comprehensive information for the analysis of bacteria in a short time and with high sensitivity. Raman spectroscopy offers many advantages including relatively simple operation, non‐destructive analysis, and information on molecular differences between bacteria species and strains. A variety of biochemical properties can be measured in a single spectrum. This short review covers the recent advancements and applications of Raman spectroscopy for bacteria analysis with specific focuses on bacteria detection, bacteria identification and discrimination, as well as bacteria antibiotic susceptibility testing in 2022. The development of novel substrates, the combination with other techniques, and the utilization of advanced data processing tools for the improvement of Raman spectroscopy and future directions are discussed.

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Section: Different Bacterial Responses During Antibiotics Exposurementioning

confidence: 99%

Recent advances of Raman spectroscopy for the analysis of bacteria

Rodriguez

Zhang

Wang

2023

Analytical Science Advances

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“…Tian et al described nanoparticle arrays for the simultaneous capture, identification, and differentiation of Gram-positive and Gram-negative bacteria [93]. Apart from identifying bacteria in different samples, it is possible to use SERS to differentiate between bacterial strains that are sensitive or resistant to ampicillin [94]. Gukowsy et al tested three approaches (a conventional SERS approach of mixing a droplet of bacterial culture with gold nanoparticles, extracellular matrix analysis, and in situ mapping of bacterial cells on a filter membrane), where the last two methods proved more effective [94].…”

Section: Detection Of Bacteriamentioning

confidence: 99%

How Surface-Enhanced Raman Spectroscopy Could Contribute to Medical Diagnoses

et al. 2022

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In the last decade, there has been a rapid increase in the number of surface-enhanced Raman scattering (SERS) spectroscopy applications in medical research. In this article we review some recent, and in our opinion, most interesting and promising applications of SERS spectroscopy in medical diagnostics, including those that permit multiplexing within the range important for clinical samples. We focus on the SERS-based detection of markers of various diseases (or those whose presence significantly increases the chance of developing a given disease), and on drug monitoring. We present selected examples of the SERS detection of particular fragments of DNA or RNA, or of bacteria, viruses, and disease-related proteins. We also describe a very promising and elegant ‘lab-on-chip’ approach used to carry out practical SERS measurements via a pad whose action is similar to that of a pregnancy test. The fundamental theoretical background of SERS spectroscopy, which should allow a better understanding of the operation of the sensors described, is also briefly outlined. We hope that this review article will be useful for researchers planning to enter this fascinating field.

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“…In addition, the effect of antibiotics and/or antibiotic susceptibility can be recognized by the altered spectra of metabolites secreted by bacteria. Therefore, there are two approaches to study the antibiotic effect on bacteria—direct investigation of bacterial cells (either in suspension or on the solid support) and detection of secreted metabolites (for example, by studying the extracellular matrix) [ 88 ].…”

Section: Antibiotic Susceptibilitymentioning

confidence: 99%

“…The SERS signal of sensitive bacteria responds more dramatically to antibiotic treatment than that of their resistant counterparts [ 88 , 94 ]. The dependency of Raman shift intensity of antibiotic-susceptible species of E. coli via antibiotic concentration achieves the maximum, with the maximum intensity being achieved at the minimum inhibitory concentration (MIC), while the Raman signal of antibiotic resistant strain slightly depends on the antibiotic concentration [ 91 ].…”

Section: Antibiotic Susceptibilitymentioning

confidence: 99%

“…Several SERS-based approaches, including the conventional drop method, extracellular matrix analysis, and filter mapping for investigating E. coli O157:H7 susceptibility to ampicillin, were studied in [ 88 ]. Bacteria are differentiated by observing a peak, the intensity of which increases due to lysis of the bacterium by the antibiotic treatment and the release of intracellular purine compounds.…”

Section: Antibiotic Susceptibilitymentioning

confidence: 99%

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Antibiotic Susceptibility Testing with Raman Biosensing

et al. 2022

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Antibiotics guard us against bacterial infections and are among the most commonly used medicines. The immediate consequence of their large-scale production and prescription is the development of antibiotic resistance. Therefore, rapid detection of antibiotic susceptibility is required for efficient antimicrobial therapy. One of the promising methods for rapid antibiotic susceptibility testing is Raman spectroscopy. Raman spectroscopy combines fast and contactless acquisition of spectra with good selectivity towards bacterial cells. The antibiotic-induced changes in bacterial cell physiology are detected as distinct features in Raman spectra and can be associated with antibiotic susceptibility. Therefore, the Raman-based approach may be beneficial in designing therapy against multidrug-resistant infections. The surface-enhanced Raman spectroscopy (SERS) and resonance Raman spectroscopy (RRS) additionally provide excellent sensitivity. In this review, we present an analysis of the Raman spectroscopy–based optical biosensing approaches aimed at antibiotic susceptibility testing.

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Assessment of three SERS approaches for studying E. Coli O157:H7 susceptibility to ampicillin

Cited by 8 publications

References 36 publications

Recent advances of Raman spectroscopy for the analysis of bacteria

Recent advances of Raman spectroscopy for the analysis of bacteria

How Surface-Enhanced Raman Spectroscopy Could Contribute to Medical Diagnoses

Antibiotic Susceptibility Testing with Raman Biosensing

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