Assessment of vinyl chloride-induced DNA damage in lymphocytes of plastic industry workers using a single-cell gel electrophoresis technique

Awara, Wageh M.; El-Nabi, Sobhy Hassab; El-Gohary, Mona

doi:10.1016/s0300-483x(98)00008-0

Cited by 45 publications

(16 citation statements)

References 19 publications

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“…Increased DNA damage by older age as detected by the comet assay was also reported in other studies [46][47][48][49][50][51]. In contrast, there are studies that showed no effect of age on the level of DNA damage [52][53][54][55][56][57][58]. As the children in our study were in the similar age range spreading over only ten years it was difficult to determine if there is an actual age-difference in DNA damage among them.…”

Section: Discussionsupporting

confidence: 48%

Cytogenetic status of healthy children assessed with the alkaline comet assay and the cytokinesis-block micronucleus cytome assay

Gajski

Gerić

Oreščanin

et al. 2013

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Section: Discussionsupporting

confidence: 48%

Cytogenetic status of healthy children assessed with the alkaline comet assay and the cytokinesis-block micronucleus cytome assay

Gajski

Gerić

Oreščanin

et al. 2013

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…3 The comet assay has been extensively used for human biomonitoring studies. Lymphocytes are the preferred cell type as they are an internationally accepted indicator tissue to asses the consequence of human exposure to genotoxic hazard 4,5 and it is a simple, sensitive, rapid technique for the assessment of DNA damage and also they allow the assay to be non-invasive.…”

Section: Introductionmentioning

confidence: 99%

Total antioxidant status and oxidative DNA damage in a South Indian population of essential hypertensives

et al. 2010

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To investigate the total antioxidant status (TAS) and the extent of oxidative DNA damage in total lymphocytes and their relation with essential hypertension. A total of 130 South Indian subjects aged 30-65 were recruited for the study. Of these hypertensive subjects investigated, 30 were newly diagnosed and were not on any antihypertensive drugs, but had systolic blood pressure (BP) ranging between 140 and 160 mm Hg and diastolic BP between 95 and 100 mm Hg; 50 hypertensive patients who were already on drug therapy for 1 year and 50 were normotensive controls with BP p120/80 mm Hg. DNA damage was significantly increased in hypertensive patients (both newly diagnosed and who were already on drug therapy) compared with control group. The major increase in DNA damage was observed in newly diagnosed hypertensive patients compared with hypertensive patients who were already on drug therapy. There was a significant decrease in plasma TAS value in essential hypertensive groups as compared to normotensive controls. Lymphocyte DNA damage was independently correlated with only TAS. Lymphocyte DNA damage was increased in hypertensive patients. The major increase in lymphocyte DNA damage was observed in newly diagnosed hypertensive patients compared with hypertensive patients who already on drug therapy. Decreased TAS levels, which reflect to increased oxidative stress, may be the reason of increased total lymphocyte DNA damage in South Indian hypertensive patients.

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“…The assay is increasingly used in biomonitoring and epidemiological studies [Betti et al, 1994[Betti et al, , 1995Collins et al, 1997;Kassie et al, 2000;Dhawan et al, 2001;Bajpayee et al, 2002;Faust et al, 2004], where it is most often used to detect DNA damage in peripheral lymphocytes. Using the Comet assay, higher levels of DNA damage have been reported among persons exposed to a variety of chemicals [Hartmann et al, 1995;Yuspa and Shields, 1997;Awara et al, 1998;Andreoli et al, 1999;Lei et al, 2004] and ionizing radiation [Singh et al, 1995;Nascimento et al, 2001]. The DNA damage detected by the Comet assay also has been reported to vary with gender [Bajpayee et al, 2002], age [Singh et al, 1990[Singh et al, , 1991, habits, and lifestyle [Betti et al, 1994[Betti et al, , 1995Piperakis et al, 1998;Dhawan et al, 2001;Park et al, 2003;Hininger et al, 2004].…”

Section: Introductionmentioning

confidence: 99%

DNA damage in lymphocytes of rural Indian women exposed to biomass fuel smoke as assessed by the Comet assay

Pandey

Bajpayee

Parmar

et al. 2005

Environ. Mol. Mutagen.

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The Comet assay has found wide acceptance in monitoring human genotoxicity caused by lifestyle and occupational and environmental factors. In the present study, we have used the Comet assay to measure the DNA damage in a population of rural Indian women cooking with biomass fuels (BMFs; fire wood and cow dung cakes). Out of 144 volunteers, 70 used BMFs for domestic cooking, while the remaining 74 used liquefied petroleum gas (LPG) and served as a reference population. All the individuals had comparable socioeconomic backgrounds and were between 20 and 55 years of age. Significantly higher levels of DNA damage were observed for BMF users than for LPG users. For BMF users in comparison with the reference population, Olive tail moment was 3.83 +/- 0.15 (arbitrary units) vs. 2.77 +/- 0.07 (P < 0.001); % tail DNA was 11.19 +/- 0.35 vs. 8.29 +/- 0.20 (P < 0.001); and comet tail length (microm) was 51.15 +/- 1.37 vs. 40.26 +/- 0.88 (P < 0.001). Similar significant differences were found when the groups were stratified by age and length of exposure. This study suggests that exposure to BMF smoke leads to greater levels of DNA damage than exposure to LPG combustion products.

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Assessment of vinyl chloride-induced DNA damage in lymphocytes of plastic industry workers using a single-cell gel electrophoresis technique

Cited by 45 publications

References 19 publications

Cytogenetic status of healthy children assessed with the alkaline comet assay and the cytokinesis-block micronucleus cytome assay

Cytogenetic status of healthy children assessed with the alkaline comet assay and the cytokinesis-block micronucleus cytome assay

Total antioxidant status and oxidative DNA damage in a South Indian population of essential hypertensives

DNA damage in lymphocytes of rural Indian women exposed to biomass fuel smoke as assessed by the Comet assay

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