Association between FASN gene polymorphisms ultrasound carcass traits and intramuscular fat in Qinchuan cattle

Raza, Sayed Haidar Abbas; Gui, Linsheng; Khan, Rajwali; Schreurs, Nicola M.; Wang, Xiaoyu; Wu, Sen; Mei, Chugang; Wang, Li; Ma, Xiaoxing; Wei, Dawei; Guo, Hongwei; Song, Zhijian; Wang, Xingping; Kaleri, Hubdar Ali; Liu, Zan

doi:10.1016/j.gene.2017.12.034

Cited by 51 publications

(35 citation statements)

References 28 publications

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“…Grzes et al [17] showed Other studies also reported the relationship between FASN mutations and backfat thickness [18,33]. In cattle, FASN gene polymorphisms were associated with subcutaneous fat and IMF content [34,35]. Authors [34,35] showed that FASN g.13232C > T mutation significantly increases IMF level (+7.83%) in Qinchuan cows.…”

Section: Discussionmentioning

confidence: 97%

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Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq Results

Piórkowska

Małopolska

Ropka‐Molik

et al. 2020

Animals

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In recent years, pig producers have struggled with the problem of low intramuscular fat levels in pork, which impacts palatability and ultimately meat quality. Reduced levels of intramuscular fat are likely the result of breeding objectives aimed at increasing lean meat content. In this study, three mutations within candidate genes for fat content (SCD, ACACA, and FASN) were selected, based on RNA-seq results and the relationship between polymorphisms in genes related to lipid metabolism, fattening and slaughter characteristics, as well as pork quality, including IMF level, were evaluated to identify selection markers. Moreover, their impact on gene expression was also examined. The PCR–RFLP (polymerase cha- in reaction – restriction fragments length) method was used to establish genotypes and effect sizes of potential genetic markers were estimated using a GLM model. It was identified that a FASN missense variant was positively associated with the expression level of this gene, which suggested its linkage with a mutation having a regulatory function. The association study indicated that the FASN missense variant may play a role in the determination of feed conversion and meat colour. In turn, a mutation in the ACACA gene showed a relationship with IMF content in the Puławska breed where the differences reached as much as 20%. We suggest considering all three mutations in further studies based on different pig populations due to the crucial role of SCD, ACACA, and FASN genes in lipid metabolism.

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Section: Discussionmentioning

confidence: 97%

“…In cattle, FASN gene polymorphisms were associated with subcutaneous fat and IMF content [34,35]. Authors [34,35] showed that FASN g.13232C > T mutation significantly increases IMF level (+7.83%) in Qinchuan cows. Unfortunately, our analysis did not indicate any relation between IMF and FASN c.1328G > A mutation.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq Results

Piórkowska

Małopolska

Ropka‐Molik

et al. 2020

Animals

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“…The loin area muscle and intramuscular fat contents are the key indicators of meat quality grading. These traits are mostly affected by the age of the animals, management conditions such as nutrition and by the genetics of the animals [15,16]. To achieve sustainable improvement in these traits of economic importance, selective breeding is an effective strategy, but it takes a very long time to obtain efficient genetic gain due to the longer generation interval in cattle.…”

Section: Introductionmentioning

confidence: 99%

Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle

et al. 2020

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The TORC2 gene is responsible for nutrient metabolism, gluconeogenesis, myogenesis and adipogenesis through the PI3K-Akt, AMPK, glucagon and insulin resistance signaling pathways. Sequencing of PCR amplicons explored three novel SNPs at loci g.16534694G>A, g.16535011C>T, and g.16535044A>T in the promoter region of the TORC2 gene in the Qinchuan breed of cattle. Allelic and genotypic frequencies of these SNPs deviated from Hardy-Weinberg equilibrium (HWE) (P < 0.05). SNP1 genotype GG, SNP2 genotype CT and SNP3 genotype AT showed significantly (P <0.05) larger body measurement and improved carcass quality traits. Haplotype H1 (GCA) showed significantly (p<0.01) higher transcriptional activity (51.44%) followed by H4 (ATT) (34.13%) in bovine preadipocytes. The diplotypes HI-H3 (GG-CC-AT), H1-H2 (GG-CT-AT) and H3-H4 (GA-CT-TT) showed significant (P<0.01) associations with body measurement and improved carcass quality traits. Analysis of the relative mRNA expression level of the TORC2 gene in different tissues within two different age groups revealed a significant increase (P<0.01) in liver, small intestine, muscle and fat tissues with growth from calf stage to adult stage. We can conclude that variants mapped within TORC2 can be used in marker-assisted selection for carcass quality and body measurement traits in breed improvement programs of Qinchuan cattle.

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“…The greatest differences in the physical phenotypes of these two breeds are their body size and adaptation to different environments. There are many genes that have been reported to be associated with the economic traits of cattle (Li et al, 2010;Pausch et al, 2017;Raza et al, 2018;Sun et al, 2018;Cheng et al, 2020). In particular, genes such as MYLK4 (Zheng et al, 2019), CRTC3 (Wu et al, 2018), LEPR (Guo et al, 2008), and LHX4 (Ren et al, 2010) have been reported to be associated with the growth traits of Chinese cattle breeds.…”

Section: Discussionmentioning

confidence: 99%

Whole Transcriptome Analysis Identifies the Taxonomic Status of a New Chinese Native Cattle Breed and Reveals Genes Related to Body Size

et al. 2020

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Wandong (WD) cattle has recently been identified as a new Chinese native cattle breed by the National Commission for Livestock and Poultry Genetic Resources. The population size of this breed is less than 10,000. WD cattle and Dabieshan (DB) cattle are sympatric but are raised in different ecological environments, on mountains and plains, respectively, and the body sizes of these two breeds are markedly different. Blood samples were obtained from 8 adult female WD cattle and 7 adult female DB cattle (24 months old). The total RNA was extracted from leukocyte cells, and sequencing experiments were conducted on the Illumina HiSeq TM 4000 platform. After the removal of one outlier sample from the WD cattle breed as determined by principal component analysis (PCA), phylogenetic and population structure analyses indicated that WD and DB cattle formed a distinct Central China cattle group and showed evidence of hybridization between Bos. taurus and Bos. indicus. The immune-regulator CD48 (P = 1.3E-6) was associated with breed-specific traits according to loss-offunction variant enrichment analysis. In addition, 113 differentially expressed genes were identified between the two breeds, many of which are associated with the regulation of body growth, which is the major difference between the two breeds. This study showed that WD cattle belong to the group of hybrids between Bos. Taurus and Bos. indicus, and one novel gene associated with breed traits and multiple differentially expressed genes between these two closely related breeds was identified. The results provide insights into the genetic mechanisms that underlie economically important traits, such as body size, in cattle.

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Association between FASN gene polymorphisms ultrasound carcass traits and intramuscular fat in Qinchuan cattle

Cited by 51 publications

References 28 publications

Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq Results

Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq Results

Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle

Whole Transcriptome Analysis Identifies the Taxonomic Status of a New Chinese Native Cattle Breed and Reveals Genes Related to Body Size

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